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Amino acid sequences of eukaryotic translation elongation factor isoform 1 (eEF1A1) and 2 (eEF1A2) were compared and two peptide fragments of eEF1A2 were chosen as linear antigenic determinants for generation of monospecific antipeptide antibodies. Selected peptides were synthesized, conjugated to bovine serum albumin (BSA) and used for mice immunizations. Antibodies, produced against the eEF1A2 fragment 330-343 conjugated to BSA, specifically recognized this isoform in the native and partially denatured states but did not interact with the eEF1A1 isoform. It was shown that these monospecific anti-eEF1A2 antibodies could be employed for eEF1A2 detection both by enzyme-linked immunosorbent assay and by immunoblotting.

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The properties and conditions of Jabrication of spinning solutions based on polyvinyl alcohol containing the enzvme plvtease C, a high-molecular-weight antimicrobial, polyhexamethyleneguanidine hydrochloride, and modifvhzg additives of difJerent kinds --the polysaccharide sodium alginate and sodium tetraborate, were investigated. A correlation was established between the proteolytic activity of the film materials, degree of swelling, and composition of the spinning solutions used to prepare them.An important direction in the chemistry and technology of the fabrication and use of polymers in medicine is the creation of a new generation of dressings with special properties defined by their application. As a function of the wound etiology and individual course of the wound process, polymeric coatings which have both a high and a low level of sorptivity, are biodegradable, and on the contrary, stable in vivo, simply isolate the wound from the outside, and also have a medicinal action, are required [I ]. Polymeric materials with therapeutic properties, in particular, enzymatic and antimicrobial actions, are considered the most promising, The problem of fabricating such materials can be solved by combined immobilization ofa proteolytic enzyme and an antimicrobial in the structure of the polymeric material. In our previous studies [2], we showed that selection of both the method of immobilization and the polymer systems allows regulating the level of biological activity and stability of the materials obtained. For fabricating polyfunctional biologically active materials, we used the method of incorporating a proteolytic enzyme, Terrilytin, or protease C (Pr), and a high-molecular-weight antimicrobial, polyhexamethyleneguanidine hydrochloride (PHMG), in the structure ofa polyvinyl alcohol (PVA) film [3].The use of modifying additives of different kinds which not only affect the properties of biologically active substances but also the structure of the polymer matrix provides extensive possibilities for regulating the properties of enzyme-containing film materials [2]. A polysaccharide containing ionogenic groups, sodium alginate (Alg), and a cross-linking reagent, sodium tetraborate, Na2B4OT, were used as the modifying reagents.In fabricating film coatings containing biologically active substances of different natures and modifying additives, it is extremely important to assess the possibility of fabricating kinetically stable spinning solutions which retain the necessary level of biological properties. Since polyelectrolytes from different classes (Pr --a polyampholyte, Alg --a polyacid sodium salt, PHMG--a polybase) were the components of the spinning composite, the characteristics of their reaction in aqueous solution and the properties of the enzyme were investigated first to determine the conditions of fabrication of the spinning composites: concentration limits, order of addition of components, duration of storage of solutions.The change in the viscosity and in some cases phase separation of the reaction mixture ob...