Pharmacokinetic Properties of PVA Films with Combined Biological Action

Yudanova, T. N.; Aleshina, E. Yu.; Гальбрайх, Л. С.; Krest'yanova, I. N.

doi:10.1023/b:phac.0000016070.10440.9c

Cited by 3 publications

(2 citation statements)

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“…This composition was capable of converting into a gel and incorporating 0.05–0.20 ml/cm 2 of a bacteriophage mixture. In this preparation, PVA — a hydrophilic biocompatible polymer — serves as a suitable matrix for the immobilization of bioactive substances [ 5 , 8 ]. The role of PB as a component of the film is to create an optimal pH for maintaining the viability of bacteriophages.…”

Section: Methodsmentioning

confidence: 99%

Viability of Bacteriophages in the Complex Hydrogel Wound Dressings in vitro

Бесчастнов¹,

Ryabkov²,

Leontiev³

et al. 2021

Sovrem Tehnol Med

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Using bacteriophages to overcome the increasing resistance of microorganisms to antibiotics is a novel research venue of clinical importance. Among other challenges, this technique is expected to create and maintain an adequate local concentration of bacteriophages at the site of application. In addition, the possibility of combining the phage preparation with antioxidants and anesthetics may provide new options for stimulating the reparative process.The aim of the study was to assess the viability and lytic activity of bacteriophages incorporated into a hydrogel-based wound dressing that contains polyvinyl alcohol, phosphate buffer, with optional additions of succinic acid and lidocaine. Materials and Methods.A technique for incorporating bacteriophages into the complex hydrogel wound dressing ex tempore has been proposed. The bacteriolytic activity of phages inside the hydrogel was determined using standard microbiological techniques. Specifically, we used nutrient media with lawn cultures of Staphylococcus aureus added with the following antibacterial combinations: bacteriophages + succinic acid, bacteriophages + lidocaine, and bacteriophages + succinic acid + lidocaine. The lytic activity of bacteriophages was assessed within 1 to 7 days after the formation of the hydrogel.Results. In all samples containing bacteriophages, the presence of viable and lytically active phages was noted within 1 to 7 days, as evidenced by the "negative colonies" on the culture lawns. On days 1 to 3, no secondary growth was recorded in the phage-containing samples. In hydrogel samples containing phages, succinic acid, and lidocaine, secondary bacterial colonies were detected starting from day 4 indicating some reduction in the lytic activity. Conclusion.The results suggest that bacteriophages immobilized in the hydrogel maintain their viability and lytic activity, and this activity persists when the phages are combined with succinic acid and lidocaine.

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Section: Methodsmentioning

confidence: 99%

Viability of Bacteriophages in the Complex Hydrogel Wound Dressings in vitro

Бесчастнов¹,

Ryabkov²,

Leontiev³

et al. 2021

Sovrem Tehnol Med

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“…Особенный интерес представляют порфиринаты металлов как модификаторы полимерных систем. С их помощью изготавливают по-лимерные пленки монолитного типа медико-биологического назначения, обладающие антимикробным, протеолитическим и комбинированным действием [12], а также пленочные материалы с контролируемым выделением биологически активных веществ [13], электроизоляционные органические полимерные пленки [14], простые однослойные пленки, многослойные пленки, полимерные мембраны [2,15] и т.д. Известно, что порфиринаты металлов способны влиять на влагопроницаемость пленок, селективность, чувствительность к различным низкомолекулярным соединениям [16,17], термоокислительную устойчивость поливинилхлоридного пластиката [18].…”

Section: Introductionunclassified

Spectral Properties of Zinc and Cobalt Meso-Tetraphenylporphyrins in Polymer Films

Glazkova¹,

Petrova²,

Rodina³

et al. 2020

IVKKT

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The results of studying the spectral properties of zinc and cobalt meso-tetraphenyl-porphyrins included as the modifiers in polymethylmethacrylate, polystyrene and polyvinylchloride films are presented in this article for the first time. It was established that an inert polymer matrix of polyvinylchloride, block polystyrene and polymethylmethacrylate does not affect the spectral properties of tetrapyrrole macroheterocycles, which are included in it. In this case, insignificant bathochromic shift of the absorption bands of the metal complexes in the modified films is recorded, which is probably due to the presence of intermolecular interaction of the metalloporphyrin with the polymer matrix. The method of polymer producing is important for modification of polystyrene or polymethyl methacrylate films. The ignificant changes are observed in the electronic spectra of metalloporphyrins due to the interaction between the modifier and the residual amount of the radical polymerization initiator (benzoyl peroxide) used in the preparation of polymers. Earlier studies showed that the interaction of benzoyl peroxide with zinc and cobalt meso-tetraphenylporphyrinates in solution leads to the formation of the corresponding metalloisoporphyrinates. In this work, the possibility of such structures formation in a polymer matrix is shown for the first time. In this case, in the electronic absorption spectra of the modified films, as in solutions, bands appear in the near IR region. The fluorescent properties of modified films have been studied. It was shown that in the absence of interaction between the components in the film, metalloporphyrins introduced into the polymer matrix retain their ability to fluorescence.

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The use of bacteriophages for prevention of infections in the surgical area at free skin grafting

et al. 2019

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Цель исследования: профилактика инфекционных осложнений в области кожной пластики путем иммобилизации актуальных для данной медицинской организации бактериофагов в области реципиентной раны. Материалы и методы. Разработан способ кожной пластики, при котором бактериофаги иммобилизуются в заданной области при помощи гидрогелевой повязки, приготавливаемой ex tempore. Для контроля жизнеспособности и эффективности бактериофагов, находящихся в гидрогелевой повязке, выполнили бактериологические исследования in vitro и in vivo. Результаты. Выявлено, что бактериофаги сохраняют жизнеспособность, биологическую доступность и литическую активность в гидрогелевой повязке в течение 4-5 суток. Подтверждена возможность и безопасность использования бактериофагов для защиты расщепленного аутодермотрансплантата от госпитального инфицирования. Выводы. Использование бактериофагов для профилактики местных инфекционных осложнений при свободной кожной пластике перспективно и технически возможно. Ключевые слова: свободная кожная пластика, рана, микрофлора, бактериофаги.

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Pharmacokinetic Properties of PVA Films with Combined Biological Action

Cited by 3 publications

References 0 publications

Viability of Bacteriophages in the Complex Hydrogel Wound Dressings in vitro

Viability of Bacteriophages in the Complex Hydrogel Wound Dressings in vitro

Spectral Properties of Zinc and Cobalt Meso-Tetraphenylporphyrins in Polymer Films

The use of bacteriophages for prevention of infections in the surgical area at free skin grafting

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