The main directions of research aimed at the development of new wound dressings are considered. An important modern trend is the use of biocompatible natural and synthetic polymers and their compositions as the bases of wound dressings. The new products are free of the disadvantages of traditional textile materials, have flexible design, and possess combined properties (including antimicrobial activity), which expands their functions. Important advantages of new dressings are the atraumatic character, effective curative action, and reduced therapy time.
850091-150X/06/4002-0085
E. N. Milyukina, and L. S. Gal'braikhIt was found that the stability of trypsin is higher than the stability of terrilytin in immobilization on cellulose or polyester fi bre material in a composite with polyethylene oxide with a molecular weight of 1.5, 4, or 6 kDa. The optimum compositions are cellulose and polyester materials containing terrilytin or trypsin and 2.5% PEO with MW of 6 kDa or trypsin and 0.5% PEO with MW of 1.5 kDa. The fi bre materials containing immobilized terrilytin with polyethylene oxide are resistant to radiation sterilization. The activity increases by 2-4 times in comparison to the initial materials in storage of sterilized samples at room temperature for 2 years.The variety of forms of medical dressings (gels, fi lms, powders, etc.) was obtained by expanding the arsenal of synthetic and natural polymers used to make them [1]. However, as previously, fi bre materials are an enormous proportion of the total volume of wound dressings. Fibres with high biocompatibility -cellulose and polyester -are among the most widely used fi bres for fabricating dressings. They are convenient matrices for biologically active compositions, while the activity level can be regulated by changing the composition of the polymer composite layer. Polyvinyl alcohol, sodium alginate, and chitin and chitosan derivatives can be components of polymer systems containing an enzyme and an antimicrobial [2]. A drawback of these polymers is the limitation on the concentration, since application on fi bres in an amount greater than the optimum amount signifi cantly increases the rigidity of the materials in the dry state. This drawback can be eliminated when other polymers are used, polyethylene oxide (PEO), for example, which is widely used in production of different drugs [3]. Polyethylene oxide is an amphiphilic polymer capable of forming covalent and noncovalent complexes with proteins [4]. We investigated the properties trypsin in PEO solution previously [5], but the characteristics change signifi cantly when it is included in a fi bre support [2].The characteristics of immobilization of enzymes with proteolytic activity in composites with PEO in the structure of fi bre materials -cellulose (CM) or polyester (PEM) -were investigated to create fi bre materials with biological activity.The objects investigated were fabricated by treating the fi bre materials with solutions containing PEO with a molecular weight (MW) of 1.5, 4, or 6 kDa (BASF, Germany) and protease -trypsin or terrilytin. The activity of the enzymes was analyzed with respect to casein according to Hammarsten [6]. The relative activity, equal to the ratio of the activities of immobilized and native enzymes, expressed in percentage, was calculated to characterize the activity of the immobilized enzyme. The stability of the enzyme was characterized by the residual activity after incubation of the material in physiological saline (0.9% NaCl solution) for different times in model conditions: modulus of 30, temperature of 37°C.The surface of the samples was in...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.