A new series of multidentate copper(ii) complexes [Cu(L1−5)](ClO4) (1–5) were synthesized and characterized for their DNA/BSA binding, DNA cleavage, cytotoxic properties and antimicrobial activities.

Presently, quite a lot of research that are being carried out to find a potential cure for cancer and many had made to clinical trial stage as well. In the present study, we focus on use of a novel graphene oxide functionalized chitosan nanoparticle targeting Saos-2 and MG-63 osteosarcoma cells. The graphene oxide chitosan nanoparticles were loaded with siRNA, studied for in vitro release with varying concentration & pH, and fitted to peppas model. MTT & ROS assay was used to evaluate biocompatibility of carrier and qPCR to study the inflammatory responses in particular checking gene expression of IL-6, TGF-ß, TNF-α in both RAW 264.7 and bone marrow derived macrophages. The results of study showed that release of siRNA were in a controlled fashion and effective at acidic pH that prevails on tumor site. The material was biocompatible and effective in case of Saos-2 osteosarcoma cells with a viability of 0.4±0.43 and 0.49±0.53 in case of MG-63 cells when treated with highest concentration of 100µl siRNA compared to untreated cells that were in range of 0.64±0.67 in Saos-2 and 0.61±0.63 in MG-63 cells. The results of expression of inflammatory cytokines IL-6, TGF-β & TNF-α showed negligible amount compared to control group serving the purpose of an effective carrier targeting tumor cells. Highlights  Graphene oxide functionalized chitosan nanoparticle loaded with siRNA targeting Saos-2 and MG-63 osteosarcoma cells exhibited a controlled release.  Effective release of siRNA on cancer cells and destruction of the same.  No inflammation observed when treated with RAW and Bone Marrow derived macrophages derived from mice models.

Practical implementation of photocatalytic hydrogen production has been required for the invention of efficient visible light absorption and low cost photocatalyst materials.

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BackgroundThe application of polymeric materials in medical industry has grown drastically in the last two decades due to their various advantages compared to existing materials. The present research work emphases on the sol-gel technique to formulate the polymethyl methyl acrylate/polystyrene/silica composite membrane.MethodsThe characteristic of the composite was investigated through modern state art of instrumentation.ResultsThe functional groups attached to the polymer was absorbed by FTIR. The FTIR spectrum confirm that the blend was mixed thoroughly and the formation of unite intimately between the polymers. The membranes were observed by SEM for its surface homogeneity which depends upon the composition of the two blending polymers. The captured SEM images showed the formation of microcracks on the surface, which was evidently controlled by varying the constituent polymer ratios. The prepared blend membranes with 2:1 ratio of PMMA/PS/Si displayed higher water uptake compared to other blended membranes. The composite membranes had good hydroxyl apatite growth in SBF solution. Furthermore, the in vitro cytotoxicity studies carried out by MTT method, using RAW macrophage cells showed that all the samples exhibited excellent cell viability.ConclusionThe inflammatory response of composite with equal concentration of PMMA-PS were performed and observed no inflammation in comparison with control and other tested concentrations.

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