A new series of multidentate copper(ii) complexes [Cu(L1−5)](ClO4) (1–5) were synthesized and characterized for their DNA/BSA binding, DNA cleavage, cytotoxic properties and antimicrobial activities.

Presently, quite a lot of research that are being carried out to find a potential cure for cancer and many had made to clinical trial stage as well. In the present study, we focus on use of a novel graphene oxide functionalized chitosan nanoparticle targeting Saos-2 and MG-63 osteosarcoma cells. The graphene oxide chitosan nanoparticles were loaded with siRNA, studied for in vitro release with varying concentration & pH, and fitted to peppas model. MTT & ROS assay was used to evaluate biocompatibility of carrier and qPCR to study the inflammatory responses in particular checking gene expression of IL-6, TGF-ß, TNF-α in both RAW 264.7 and bone marrow derived macrophages. The results of study showed that release of siRNA were in a controlled fashion and effective at acidic pH that prevails on tumor site. The material was biocompatible and effective in case of Saos-2 osteosarcoma cells with a viability of 0.4±0.43 and 0.49±0.53 in case of MG-63 cells when treated with highest concentration of 100µl siRNA compared to untreated cells that were in range of 0.64±0.67 in Saos-2 and 0.61±0.63 in MG-63 cells. The results of expression of inflammatory cytokines IL-6, TGF-β & TNF-α showed negligible amount compared to control group serving the purpose of an effective carrier targeting tumor cells. Highlights  Graphene oxide functionalized chitosan nanoparticle loaded with siRNA targeting Saos-2 and MG-63 osteosarcoma cells exhibited a controlled release.  Effective release of siRNA on cancer cells and destruction of the same.  No inflammation observed when treated with RAW and Bone Marrow derived macrophages derived from mice models.

Practical implementation of photocatalytic hydrogen production has been required for the invention of efficient visible light absorption and low cost photocatalyst materials.

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