Banan peel of Agung Semeru and banana peel of Mas Kirana, is one part of banana fruit containing antimicrobial compounds that contain phytochemical components. The purpose of this research is to know the quality of cream from banana peel extract of Agung Semeru, Mas Kirana and combination of both using 100% concentration (the best concentration, based on previous research results). In the test the cream is done organoleptic test, test homogeneity, spreading test, pH test and absorption test. The results obtained on the organoleptic test seen from the color of brown cream, semi-solid dosage form, and the smell of banana skin cream typical test homogeneity does not occur agglomeration, pH ranges from 5-6 and still safe for the skin, has a cream that meets the requirements of absorption that is more 1 mg / 1 ml. A / M cream scattering test is easy to be applied so that the extract cream of skin extract of Agung Semeru banana skin extract, Mas Kirana and combination fulfill the scatter test.
Pleurotus cytidiosus has the advantage of long endurance, body texture and moisture content slightly thick. Peurotus ostreatus Grey oyster variety has the advantage of long endurance, wide body shape and also a little water content. To obtain superior variety of both species of fungus, it is necessary to cross using the monocaryon fusion mycelium. The purpose of this study to determine whether the mycelium monokaryon fusion methods can be used to cross P. cytidiosus with P. ostreatus Grey oyster variety. The results showed that the mycelium monokaryon fusion method can be used to cross P. cytidiosus with P. ostreatus Grey oyster variety marked with contact zones mycelium makroscopically, while microscopically there is a connection structure of the wedge. The characteristics of mycelium of the cross appear to be attached or united, uneven and smoother. Growth of mycelium dikaryon, faster than monokaryon mycelium.Keywords: Monokaryon Mycelium Fusion, Pleurotus. cytidiosusy, Pleurotus ostreatus Grey oyster Variety ABSTRAK Jamur tiram coklat (Pleurotus. cytidiosus) memiliki keunggulan daya tahan lama, tekstur tubuh yang tebal, dan kadar air sedikit. Jamur tiram putih (Pleurotus ostreatus varietas Grey oyster) memiliki keunggulan daya tahan lama, bentuk tubuh lebar, dan kadar air sedikit. Untuk mendapatkan varietas unggul dari kedua spesies jamur, maka perlu dilakukan persilangan menggunakan metode fusi miselium monokarion. Tujuan penelitian ini untuk mengetahui apakah metode fusi miselium monokarion dapat digunakan untuk persilangan P. cytidiosus dengan P. ostreatus varietas Grey oyster. Tahap awal dalam penelitian ini yaitu pembibitan, kemudian dilanjutkan persilangan menggunakan metode fusi miselium monokarion hingga terbentuk zona kontak kedua miselium dan struktur sambungan apit. Hasil penelitian menunjukkan bahwa metode fusi miselium monokarion dapat digunakan untuk persilangan P. cytidiosus dengan P. ostreatus varietas Grey oyster yang ditandai dengan zona kontak miselium secara makroskopik, sedangkan secara mikroskopik terdapat struktur sambungan 11
Escherichia coli is a type of Gram-negative bacteria that is commonly found in the human digestive tract. It has been reported as one of the bacteria that has been resistant to several antibiotics. This study aims to determine four (4) types of weed extracts that are considered but have not been used as antibacterial compounds against E. coli. This experimental study used a completely randomized design with several types of plant extracts, namely Acalypha indica L., Ageratum conyzoides, Phyllanthus niruri L., and Amaranthus spinosios at various concentrations (0, 50, and 100%). The results showed that the plant extract of A. indica L. had the ability as an antibacterial against the growth of E. coli at concentrations of 50% (1.41 ± 0.12) and 100% (1.53 ± 0.01) compared to other extracts. Meanwhile, the lowest average diameter of the inhibition zone for E. coli bacteria was found in the treatment of A. spinosios grass leaf extract 50% (1.17 ± 0.05).
ABSTRAKTanaman tebu adalah keluarga poace yang berpotensi menghasilkan nilai ekonomi tinggi karena manfaatnya sebagai produk unggulan dalam menghasilkan gula serta bioetanol yang banyak digunakan oleh masyarakat luas. Untuk itu perlu upaya pemuliaan tanaman yang cepat untuk memenuhi kebutuhan pasar. Teknik kultur mikrospora merupakan aplikasi bioteknologi yang efisien dalam menghasilkan tanaman haploid. Teknik ini belum berhasil diaplikasikan pada tanaman tebu sehingga dalam penelitian ini bertujuan untuk memicu embriogenesis mikrospora tebu dengan aplikasi kultur mikrospora pada varietas bululawang. Metode yang digunakan yaitu dengan stress perlakukan perendaman mannitol 0.3M sebelum isolasi kultur pada lama perendaman yang berbeda. Analisis data menggunakan uji anova dan uji duncan. Hasil yang diperoleh yaitu Perlakuan stress yang diberikan berhasil memicu embriogenesis mikrospora dengan lama perendaman anther optimum pada 7 hari pada mannitol 0.3M Kata kunci : mikrospora uninukleat, mannitol, tanaman tebu ABSTRACT Sugar cane is a poace family that has the potential to produce high economic value because of its benefits as a superior product in producing sugar and bioethanol which is widely used by the wider community. For this reason, it is necessary to initiate plants that are fast to meet market needs. Microspora culture technique is an efficient application of biotechnology in producing haploid plants. This technique has not been successfully applied to sugarcane plants so that in this study aims to trigger microsporee sugarcane embryogenesis by application of microspore culture in Bululawang variety. The method used is stress treatment of mannitol 0.3M soaking before culture isolation at different soaking times. Data analysis using anova test and duncan test. The results obtained are stress treatments given successfully trigger microspore embryogenesis with optimum anther treatment time at 7 days in mannitol 0.3M
Tujuan penelitian untuk menguji ekstrak kulit batang nangka (Artocarpus heterophylus L.) terhadap Salmonella typhi. Penelitian ini merupakan penelitian eksperimen dengan menggunakan 4 konsentrasi perlakuan 0%, 25%, 50%, 75% dan konsentrasi 100% ekstrak kulit batang nangka terhadap bakteri Salmonella typhi. Data parameter penelitian yang diamati adalah zona bening di sekitar kertas cakram yang telah direndam dalam ekstrak kulit batang nangka dengan konsentrasi 25%, 50%, 75% dan 100%. Zona bening disekitar kertas cakram disebut Daerah Hambat Pertumbuhan (DPH). Data dianalisis secara statistik dengan menggunakan uji Kruskall-Wallis pada taraf α=0,005 dengan menggunakan software SPSS 23.0, dan uji lanjutan Duncan's untuk menguji perbedaan antar perlakuan. Hasil uji menunjukkan bahwa variasi konsentrasi ekstrak kulit batang nangka berpengaruh terhadap pertumbuhan bakteri Salmonella typhi, kemudian dari uji Duncan's juga diketahui jika perlakuan 100% memberikan efek yang paling signifikan atau berbeda nyata.
Indigenous fungi are fungi that can degrade organic compounds and make them a source of nutrition for metabolism and life so that they can to survive in various environments including environments polluted by ammonia waste from the rubber factory industry. This study was conducted to obtain fungal isolates that can survive in river water contaminated with ammonia from rubber industry waste in Jember. Isolation was obtained from river water contaminated with rubber factory waste containing ammonia and isolated using the media of Potatoes Dextrose Agar (PDA). The method used in this research is descriptive exploration, namely isolating and culturing fungi using the dilution method. The results of the isolation will identify the genus of fungi based on macroscopic and microscopic morphological characters. Data analysis was done descriptively. The results obtained 4 isolates of indigenous fungi that can degrade ammonia, namely Aspergillus sp., Fusarium sp., Penicillium sp., and Yeast groups.
Jamur tiram varietas grey oyster yang sedang dikembangkan di IKIP PGRI Jember merupakan salah satu jamur tiram hibrida yang diperoleh dari rekayasa kultur jaringan dengan persilangan secara fusi protoplasma antara jamur tiram putih (Pleurotus ostreatus) dan jamur tiram cokelat (Pleurotus cystidiosus) dan memiliki prospek kedepan yang sangat baik untuk dikembangkan, dapat memberikan banyak manfaat serta memiliki dampak positif baik bagi IKIP sendiri maupun masyarakat luas. Model usaha jamur tiram putih yang dijalankan oleh Unit IbIKK pada tahun pertama masih bertumpu pada persilangan vareietas jamur tiram, produksi baglog serta difersivikssi produk dari jamur tiram, namun untuk tahun kedua dan ketiga akan lebih bervariasi, yaitu produksi jamur varietas baru hasil persilangan secara masal, penjualan baglog, jasa paket kemitraan investasi usaha tani jamur tiram, dan budidaya jamur tiram dengan sistem kemitraan. Usaha ini juga telah menjadi sarana pembelajaran mahasiswa, dan Dosen di wilayah IKIP PGRI Jember. Keywords— Jamur Tiram varietas Grey Oyster, Kewirausahaan, skill
Oyster mushroom Pleurotus ostreatus varieties Gray oyster is often consumed by the community because it tastes delicious and contains nutrients for health but utilization for medical treatment has not been studied. The aim of this research was to analyze antimicrobial potential compounds in P. ostreatus and to investigate the inhibitory effect on the growth of Staphyllococcus aureus and Pseudomonas aeruginosa. This research was experimental type with treatment variation 0.1; 0.25; 0.5; 1; 1.5 and 2% of P. ostreatus extract, with 5 replicates. Antimicrobial potential compounds was analyzed by qualitative phytochemical test while antibacterial activity evaluated by calculating the inhibition zone diameter presented as the bright zone formed in the surroundings paper discs on bacterial cultures using solid medium. Phytochemical analysis obtained positive results for flavonoid, tannin and terpenoid compounds. The effect of mushroom extract on the bacterial growth inhibition were analyzed by One Way Anova test. The result showed that the inhibitory growth diameter for both bacteria was not significantly different. It meant that the difference in concentration variations of P. ostreatus extracts of 0.25 and 0.50 did not cause different inhibitory effects on S. aureus and P aeruginosa bacteria and 0.1% concentration was capable of causing inhibitory effect.
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