Pleurotus cytidiosus has the advantage of long endurance, body texture and moisture content slightly thick. Peurotus ostreatus Grey oyster variety has the advantage of long endurance, wide body shape and also a little water content. To obtain superior variety of both species of fungus, it is necessary to cross using the monocaryon fusion mycelium. The purpose of this study to determine whether the mycelium monokaryon fusion methods can be used to cross P. cytidiosus with P. ostreatus Grey oyster variety. The results showed that the mycelium monokaryon fusion method can be used to cross P. cytidiosus with P. ostreatus Grey oyster variety marked with contact zones mycelium makroscopically, while microscopically there is a connection structure of the wedge. The characteristics of mycelium of the cross appear to be attached or united, uneven and smoother. Growth of mycelium dikaryon, faster than monokaryon mycelium.Keywords: Monokaryon Mycelium Fusion, Pleurotus. cytidiosusy, Pleurotus ostreatus Grey oyster Variety ABSTRAK Jamur tiram coklat (Pleurotus. cytidiosus) memiliki keunggulan daya tahan lama, tekstur tubuh yang tebal, dan kadar air sedikit. Jamur tiram putih (Pleurotus ostreatus varietas Grey oyster) memiliki keunggulan daya tahan lama, bentuk tubuh lebar, dan kadar air sedikit. Untuk mendapatkan varietas unggul dari kedua spesies jamur, maka perlu dilakukan persilangan menggunakan metode fusi miselium monokarion. Tujuan penelitian ini untuk mengetahui apakah metode fusi miselium monokarion dapat digunakan untuk persilangan P. cytidiosus dengan P. ostreatus varietas Grey oyster. Tahap awal dalam penelitian ini yaitu pembibitan, kemudian dilanjutkan persilangan menggunakan metode fusi miselium monokarion hingga terbentuk zona kontak kedua miselium dan struktur sambungan apit. Hasil penelitian menunjukkan bahwa metode fusi miselium monokarion dapat digunakan untuk persilangan P. cytidiosus dengan P. ostreatus varietas Grey oyster yang ditandai dengan zona kontak miselium secara makroskopik, sedangkan secara mikroskopik terdapat struktur sambungan 11
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