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New polymeric microspheres containing azomethine (1a-1c and 2a-2c) were synthesized by condensation to compare the enzymatic properties of the enzyme glucose oxidase (GOx) and to investigate antimutagenic and antimicrobial activities.The polymeric microspheres were characterized by elemental analysis, infrared spectra (FT-IR), proton nuclear magnetic resonance spectra, thermal gravimetric analysis, and scanning electron microscopy analysis. The catalytic activity of the glucose oxidase enzyme follows Michaelis-Menten kinetics. Influence of temperature, reusability, and storage capacity of the free and immobilized glucose oxidase enzyme were investigated. It is determined that immobilized enzymes exhibit good storage stability and reusability. After immobilization of GOx in polymeric supports, the thermal stability of the enzyme increased and the maximum reaction rate (V max ) decreased. The activity of the immobilized enzymes was preserved even after 5 months. The antibacterial and antifungal activity of the polymeric microspheres were evaluated by well-diffusion method against some selected pathogenic microorganisms. The antimutagenic properties of all compounds were also examined against sodium azide in human lymphocyte cells by micronuclei and sister chromatid exchange tests. K E Y W O R D Santimicrobial property, antimutagenic effect, glucose oxidase, Polymeric microsphere, Pt 4+ -azomethine

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Modified polystyrene with Pt(IV)–azomethine (APS–Sch–Pt) was synthesized by means of condensation and demonstrated to be a promising enzyme support by studying the enzymatic properties of glucose oxidase enzyme (GOx) immobilized on it. The characteristics of the immobilized glucose oxidase (APS–Sch–Pt–GOx) enzyme showed two optimum pH values that were pH = 4.0 and pH = 7. The insertion of stable Pt(IV)–azomethine spacers between the polystyrene backbone and the immobilized GOx, (APS–Sch–Pt–GOx), increases the enzymes’ activity and improves their affinity towards the substrate even at pH = 4. The influence of temperature, reusability and storage capacity on the free and immobilized glucose oxidase enzyme was investigated. The storage stability of the immobilized glucose oxidase was shown to be eleven months in dry conditions at +4 °C.

This study investigated the antimicrobial properties of polymer‐bound Schiff bases and their Cu(II) and Ni(II) complexes. Novel polystyrene oligomer‐bound Schiff bases (FMPS‐Sch) were prepared from (4‐formyl‐3‐methoxyphenoxymethyl)polystyrene (FMPS) with cycloalkylaminothiophene derivatives and their Cu(II) and Ni(II) complexes have been synthesized and characterized. All these substances have been examined for antibacterial activity against pathogenic strains; Bacillus cereus, Listeria monocytogenes, Pseudomonas putida, Escherichia coli, Salmonella typhi H, Brucella abortus, Shigella dysenteria type 10, Micrococcu.luteus and antifungal activity against Candida albicans. Most of the studied compounds were found effective against bacteria studied and yeast. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012

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