Polystyrene Attached Pt(IV)–Azomethine, Synthesis and Immobilization of Glucose Oxidase Enzyme

Sarı, Nurşen; Antepli, Esin; Nartop, Dılek; Yetim, Nurdan Kurnaz

doi:10.3390/ijms130911870

Cited by 18 publications

(8 citation statements)

References 18 publications

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“…However, after an additional ∼300 min, the YOYO-1 fluorescence behaviour dramatically changed: immediately after the light exposure, isolated, strong diffraction-limited signals appeared indicating the potential of this preparation method for SMLM (Figure 3C ). At pH 4, GOX activity is still at ∼40% of its value at neutral pH ( 51 ), therefore GOX will keep the oxygen concentration low in a sealed sample in acidic pH. When GOX was replaced with pyranose oxidase (PO), whose product of oxygen scavenging does not have an effect on pH of the buffer ( 49 ) (Figure 3A ), the YOYO-1 signal intensity remained constant over time (Figure 3B ) and no single molecule signals could be observed (data not shown).…”

Section: Resultsmentioning

confidence: 99%

Imaging chromatin nanostructure with binding-activated localization microscopy based on DNA structure fluctuations

et al. 2017

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Advanced light microscopy is an important tool for nanostructure analysis of chromatin. In this report we present a general concept for Single Molecule localization Microscopy (SMLM) super-resolved imaging of DNA-binding dyes based on modifying the properties of DNA and the dye. By careful adjustment of the chemical environment leading to local, reversible DNA melting and hybridization control over the fluorescence signal of the DNA-binding dye molecules can be introduced. We postulate a transient binding as the basis for our variation of binding-activated localization microscopy (BALM). We demonstrate that several intercalating and minor-groove binding DNA dyes can be used to register (optically isolate) only a few DNA-binding dye signals at a time. To highlight this DNA structure fluctuation-assisted BALM (fBALM), we applied it to measure, for the first time, nanoscale differences in nuclear architecture in model ischemia with an anticipated structural resolution of approximately 50 nm. Our data suggest that this approach may open an avenue for the enhanced microscopic analysis of chromatin nano-architecture and hence the microscopic analysis of nuclear structure aberrations occurring in various pathological conditions. It may also become possible to analyse nuclear nanostructure differences in different cell types, stages of development or environmental stress conditions.

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Section: Resultsmentioning

confidence: 99%

Imaging chromatin nanostructure with binding-activated localization microscopy based on DNA structure fluctuations

et al. 2017

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“…The optimum pH values are given in Table . The pH is a significant parameter capable of altering enzymatic activities in aqueous solution . The maximum activity was obtained at pH 5.0, for the free enzyme.…”

Section: Resultsmentioning

confidence: 99%

“…Covalent bonding is a preferred immobilization method because of its potential advantages such as increased enzyme activity and preservation of its structure . In GOx, the hydroxyl groups are connected by hydrogen bonds/or coordination covalent bonds to polymeric support by acting as good chelating agents …”

Section: Introductionmentioning

confidence: 99%

Novel polymeric microspheres: Synthesis, enzyme immobilization, antimutagenic activity, and antimicrobial evaluation against pathogenic microorganisms

Nartop

Demirel

Güleç

et al. 2019

J Biochem & Molecular Tox

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New polymeric microspheres containing azomethine (1a-1c and 2a-2c) were synthesized by condensation to compare the enzymatic properties of the enzyme glucose oxidase (GOx) and to investigate antimutagenic and antimicrobial activities.The polymeric microspheres were characterized by elemental analysis, infrared spectra (FT-IR), proton nuclear magnetic resonance spectra, thermal gravimetric analysis, and scanning electron microscopy analysis. The catalytic activity of the glucose oxidase enzyme follows Michaelis-Menten kinetics. Influence of temperature, reusability, and storage capacity of the free and immobilized glucose oxidase enzyme were investigated. It is determined that immobilized enzymes exhibit good storage stability and reusability. After immobilization of GOx in polymeric supports, the thermal stability of the enzyme increased and the maximum reaction rate (V max ) decreased. The activity of the immobilized enzymes was preserved even after 5 months. The antibacterial and antifungal activity of the polymeric microspheres were evaluated by well-diffusion method against some selected pathogenic microorganisms. The antimutagenic properties of all compounds were also examined against sodium azide in human lymphocyte cells by micronuclei and sister chromatid exchange tests. K E Y W O R D Santimicrobial property, antimutagenic effect, glucose oxidase, Polymeric microsphere, Pt 4+ -azomethine

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“…The elemental analyses can be considered compatible with the chemical formulas of the compounds [23,24]. The weight average molecular weight (Mw) was suggested from element analyses.…”

Section: Characterization Of Support Nanospherementioning

confidence: 99%

Research on the Repeated Use of Novel Ferrocene-Tagged Nanomaterial for Determination of Glucose

Yetim

Özkan

Daniş

et al. 2015

International Journal of Polymeric Materials and Polymeric Biom

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A good novel double ferrocene-tagged nanomaterial is designed for glucose determination and its recycling stability. Double Ferrocene-tagged nanomaterial displays higher catalytic activity toward the glucose oxidation than non-ferrocene-tagged nanomaterial. In this study novel nanoparticles including double ferrocene, N-f2-[Bis(2-aminoethyl)amino]ethylgaminomethyl-polystyrene (2AEPS) and ferrocene aldehyde (Fc) have been synthesized by means of condensation and investigated the enzymatic properties of glucose oxidase enzyme (GOD) immobilized on there. Double ferrocene-tagged nanomaterial-GOD shows high reusability and storage capacity and fast incubation time determination of glucose. 2AEPSFc-GOD retains more than 15% of the initial activity after forty five successive cycles, which is a perfect performance.

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Polystyrene Attached Pt(IV)–Azomethine, Synthesis and Immobilization of Glucose Oxidase Enzyme

Cited by 18 publications

References 18 publications

Imaging chromatin nanostructure with binding-activated localization microscopy based on DNA structure fluctuations

Imaging chromatin nanostructure with binding-activated localization microscopy based on DNA structure fluctuations

Novel polymeric microspheres: Synthesis, enzyme immobilization, antimutagenic activity, and antimicrobial evaluation against pathogenic microorganisms

Research on the Repeated Use of Novel Ferrocene-Tagged Nanomaterial for Determination of Glucose

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