Research on the Repeated Use of Novel Ferrocene-Tagged Nanomaterial for Determination of Glucose

Yetim, Nurdan Kurnaz; Özkan, Elvan Hasanoğlu; Daniş, Büşra; Tümtürk, Hayrettin; Sarı, Nurşen

doi:10.1080/00914037.2015.1030659

Cited by 12 publications

(8 citation statements)

References 29 publications

(26 reference statements)

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“…The IR spectra of all extended secondgeneration dendrimers exhibit characteristic bands of coordination water at ca. 3300 cm -1 and 1600 cm -1 assigned to νH2O and δH2O vibrations, respectively [26,27]. These observations clearly suggest that the water molecules are coordinated to the metal ion (Fig.…”

Section: T a B L Ementioning

confidence: 76%

Preparation of ferrocene core dendrimers and immobilization of AChE for detection of diclofop-methyl herbicide

Yetim

Sarı

2019

Maced. J. Chem. Chem. Eng.

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The aim of this work was to investigate diclofop-methyl detection (found in a variety of herbicides) using novel dendrimers that have ferrocene cores, as well as their Pt(II) and Pt(IV) complexes. Novel dendrimer structures were synthesized and characterized by molar conductivity, magnetic susceptibility, FTIR, UV-Vis, 1H-NMR and LC-MS methods. Then, the AChE (acetylcholine esterase) enzyme was immobilized on the novel dendrimers, and the optimal parameters (pH, temperature, repeated use, storage stability, substrate concentration) were determined for immobilized AChE. Lastly, changes in absorbance intensity were measured, and calibration graphs were plotted that reflect the inhibition reaction of immobilized AChE with diclofop-methyl. The inhibition interaction of the enzyme immobilized to the Pt(II) ion containing dendrimers was higher than that of the Pt(IV) ion containing dendrimers.

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Section: T a B L Ementioning

confidence: 76%

Preparation of ferrocene core dendrimers and immobilization of AChE for detection of diclofop-methyl herbicide

Yetim

Sarı

2019

Maced. J. Chem. Chem. Eng.

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“…The V max is the maximum reaction rate of the enzyme and shows the affinity of the enzyme to substrate. The K m value is Michaelis‐Menten constant that shows the enzyme reaches ½ V max …”

Section: Resultsmentioning

confidence: 99%

“…Optimum pH was determined for both the free GOx and the immobilized GOx by measuring the activities of free and immobilized enzymes in buffers of different pH values ranging from 3.0 to 9.5 . For this, the buffers prepared were: pH 3.0‐4.0 (CH 3 COONa=CH 3 COOH); pH 5.0 (NaH 2 PO 4 =H 3 PO 4 ); pH 6 (Na 2 HPO 4 =NaH 2 PO 4 ); pH 7.0‐9.0 (NaH 2 PO 4 =Na 2 HPO 4 ); pH 9.0 (Na 2 B 4 O 7 =NaOH).…”

Section: Methodsmentioning

confidence: 99%

Novel polymeric microspheres: Synthesis, enzyme immobilization, antimutagenic activity, and antimicrobial evaluation against pathogenic microorganisms

Nartop

Demirel

Güleç

et al. 2019

J Biochem & Molecular Tox

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New polymeric microspheres containing azomethine (1a-1c and 2a-2c) were synthesized by condensation to compare the enzymatic properties of the enzyme glucose oxidase (GOx) and to investigate antimutagenic and antimicrobial activities.The polymeric microspheres were characterized by elemental analysis, infrared spectra (FT-IR), proton nuclear magnetic resonance spectra, thermal gravimetric analysis, and scanning electron microscopy analysis. The catalytic activity of the glucose oxidase enzyme follows Michaelis-Menten kinetics. Influence of temperature, reusability, and storage capacity of the free and immobilized glucose oxidase enzyme were investigated. It is determined that immobilized enzymes exhibit good storage stability and reusability. After immobilization of GOx in polymeric supports, the thermal stability of the enzyme increased and the maximum reaction rate (V max ) decreased. The activity of the immobilized enzymes was preserved even after 5 months. The antibacterial and antifungal activity of the polymeric microspheres were evaluated by well-diffusion method against some selected pathogenic microorganisms. The antimutagenic properties of all compounds were also examined against sodium azide in human lymphocyte cells by micronuclei and sister chromatid exchange tests. K E Y W O R D Santimicrobial property, antimutagenic effect, glucose oxidase, Polymeric microsphere, Pt 4+ -azomethine

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“…Daha sonra Bölüm 2.3'deki işlemler uygulandı ve bu işlem her ay bir defa olmak üzere oda sıcaklığında tekrarlandı. Sıcaklık ve pH, sulu çözeltide enzimatik aktiviteleri değiştirebilen önemli parametrelerdir [9,10]. Serbest ve immobilize GOx'in enzimatik aktivitesinin en yüksek olduğu değerler % bağıl aktivite olarak hesaplandı.…”

Section: İmmobilize Gox Enziminin Depolama Kararlılığıunclassified

Enzyme Immobilization and Optimization on Polymeric Nanospheres

Yetim

Özkan

Sarı

2019

Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi

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Özet: Enzimler, pek çok endüstride kullanılan, maliyetleri oldukça yüksek olan önemli materyallerdir. Katalitik tepkimelerde enzimler için şartlar uygun olsa bile yüksek sıcaklık, yüksek veya düşük pH ve organik/inorganik çözücülerin varlığında enzimler aktifliklerini kolaylıkla kaybedebilirler. İmmobilizasyon işlemi ile birlikte, enzimlerin ekonomik açıdan kullanılabilirliğini sağlamak mümkündür. Bu amaçla, (aminometil) polistiren (APS) kaynaklı nanokürelere glukoz oksidaz (GOx) enzimi immobilize edildi ve optimizasyon parametreleri (pH, sıcaklık, tekrar kullanım sayısı, depolama kararlılığı, substrat derişimi) belirlendi. Serbest ve immobilize enzim için Michaelis-Menten eşitliği ile Km ve Vmak değerleri hesaplandı. Özellikle Pt(IV) iyonu içeren immobilize enzimin substratına karşı yüksek ilgi gösterdiği tespit edildi. 12 ay sonunda immobilize enzimlerin yaklaşık % 85 aktifliklerini korudukları görüldü.Abstract: Enzymes are important materials that are used in many industries and they have a very high cost. Although the conditions for the enzymes in catalytic reactions are appropriate, enzymes can easily lose their activity in the presence of high temperature, high or low pH and organic / inorganic solvents. With the immobilization process, it is possible to ensure that the enzymes are economically usable. For this purpose, the glucose oxidase (GOx) enzyme was immobilized to aminomethyl polystyrene (APS) nanospheres and the optimization parameters (pH, temperature, reusability number, storage stability, substrate concentration) were determined. Michaelis-Menten equation for free and immobilized enzyme and Km and Vmax values were calculated. In particular, it has been found that the substrate of the immobilized enzyme containing Pt(IV) ion shows a high affinity for the substrate. After 12 months, immobilized enzymes were found to maintain about 85% of their activity.

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Research on the Repeated Use of Novel Ferrocene-Tagged Nanomaterial for Determination of Glucose

Cited by 12 publications

References 29 publications

Preparation of ferrocene core dendrimers and immobilization of AChE for detection of diclofop-methyl herbicide

Preparation of ferrocene core dendrimers and immobilization of AChE for detection of diclofop-methyl herbicide

Novel polymeric microspheres: Synthesis, enzyme immobilization, antimutagenic activity, and antimicrobial evaluation against pathogenic microorganisms

Enzyme Immobilization and Optimization on Polymeric Nanospheres

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