Influence of load on the recycling stability of nanospheres attached platinium ion for determination of glucose

Özkan, Elvan Hasanoğlu; Yetim, Nurdan Kurnaz; Nartop, Dılek; Sarı, Nurşen

doi:10.1016/j.jiec.2014.10.031

Cited by 11 publications

(7 citation statements)

References 23 publications

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“…The reason for this performance may be from mediator property of ferrocene ( Figure 4). And the result is better than the earlier works [20,[24][25][26].…”

Section: Storage Stability and Reusabilitymentioning

confidence: 60%

“…Because the active site of the enzyme contains three amino acid side chains that are intimately involved in catalysis: His516 with pKa ¼ 6.9 and Glu412 with pKa ¼ 3.4, which is hydrogen bonded to His559, with pKa > 8. The protonation of each of these residues has a strong influence on all rate constants in the catalytic mechanism [24]. We can say that His516 and His559 may be more effective than Glu412 in the catalytic mechanism.…”

Section: Influence Of Ph On the Enzyme Activitymentioning

confidence: 99%

“…The elemental analyses can be considered compatible with the chemical formulas of the compounds [23,24]. The weight average molecular weight (Mw) was suggested from element analyses.…”

Section: Characterization Of Support Nanospherementioning

confidence: 99%

“…A colorimetric method based on Trinder's reaction was used for the determination of glucose concentration [24]. The following recipe was used for free enzyme=immobilize enzyme assay: 4 mL studied buffer (pH 3-9) þ 10 mg 4-aminoantipyrene þ20 mg phenol þ0.5 mg of horse radish peroxidase (HRP) þ 0.0020 g immobilize glucose oxidase in studied buffer þ16 mg glucose.…”

Section: Studies For Biocatalysismentioning

confidence: 99%

See 3 more Smart Citations

Research on the Repeated Use of Novel Ferrocene-Tagged Nanomaterial for Determination of Glucose

Yetim

Özkan

Daniş

et al. 2015

International Journal of Polymeric Materials and Polymeric Biom

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A good novel double ferrocene-tagged nanomaterial is designed for glucose determination and its recycling stability. Double Ferrocene-tagged nanomaterial displays higher catalytic activity toward the glucose oxidation than non-ferrocene-tagged nanomaterial. In this study novel nanoparticles including double ferrocene, N-f2-[Bis(2-aminoethyl)amino]ethylgaminomethyl-polystyrene (2AEPS) and ferrocene aldehyde (Fc) have been synthesized by means of condensation and investigated the enzymatic properties of glucose oxidase enzyme (GOD) immobilized on there. Double ferrocene-tagged nanomaterial-GOD shows high reusability and storage capacity and fast incubation time determination of glucose. 2AEPSFc-GOD retains more than 15% of the initial activity after forty five successive cycles, which is a perfect performance.

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“…The reason for this performance may be from mediator property of ferrocene ( Figure 4). And the result is better than the earlier works [20,[24][25][26].…”

Section: Storage Stability and Reusabilitymentioning

confidence: 60%

Section: Influence Of Ph On the Enzyme Activitymentioning

confidence: 99%

“…The elemental analyses can be considered compatible with the chemical formulas of the compounds [23,24]. The weight average molecular weight (Mw) was suggested from element analyses.…”

Section: Characterization Of Support Nanospherementioning

confidence: 99%

Section: Studies For Biocatalysismentioning

confidence: 99%

See 2 more Smart Citations

Research on the Repeated Use of Novel Ferrocene-Tagged Nanomaterial for Determination of Glucose

Yetim

Özkan

Daniş

et al. 2015

International Journal of Polymeric Materials and Polymeric Biom

Self Cite

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“…Carbohydrates extracted/derived from natural resources have excellent biocompatibility and biodegradability, making them suitable for various medical, cosmetic, environmental, and food applications [7,8,[121][122][123][124][125][126][127][128][129][130][131][132][133][134]. The recent green chemistry has focused on natural hydrophilic polymers and developed variety of derived functional materials [135][136][137][138].…”

Section: Introductionmentioning

confidence: 99%

Recently developed applications for natural hydrophilic polymers

Halake

Kim

Birajdar

et al. 2016

Journal of Industrial and Engineering Chemistry

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Novel polymeric microspheres: Synthesis, enzyme immobilization, antimutagenic activity, and antimicrobial evaluation against pathogenic microorganisms

Nartop

Demirel

Güleç

et al. 2019

J Biochem & Molecular Tox

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New polymeric microspheres containing azomethine (1a-1c and 2a-2c) were synthesized by condensation to compare the enzymatic properties of the enzyme glucose oxidase (GOx) and to investigate antimutagenic and antimicrobial activities.The polymeric microspheres were characterized by elemental analysis, infrared spectra (FT-IR), proton nuclear magnetic resonance spectra, thermal gravimetric analysis, and scanning electron microscopy analysis. The catalytic activity of the glucose oxidase enzyme follows Michaelis-Menten kinetics. Influence of temperature, reusability, and storage capacity of the free and immobilized glucose oxidase enzyme were investigated. It is determined that immobilized enzymes exhibit good storage stability and reusability. After immobilization of GOx in polymeric supports, the thermal stability of the enzyme increased and the maximum reaction rate (V max ) decreased. The activity of the immobilized enzymes was preserved even after 5 months. The antibacterial and antifungal activity of the polymeric microspheres were evaluated by well-diffusion method against some selected pathogenic microorganisms. The antimutagenic properties of all compounds were also examined against sodium azide in human lymphocyte cells by micronuclei and sister chromatid exchange tests. K E Y W O R D Santimicrobial property, antimutagenic effect, glucose oxidase, Polymeric microsphere, Pt 4+ -azomethine

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Influence of load on the recycling stability of nanospheres attached platinium ion for determination of glucose

Cited by 11 publications

References 23 publications

Research on the Repeated Use of Novel Ferrocene-Tagged Nanomaterial for Determination of Glucose

Research on the Repeated Use of Novel Ferrocene-Tagged Nanomaterial for Determination of Glucose

Recently developed applications for natural hydrophilic polymers

Novel polymeric microspheres: Synthesis, enzyme immobilization, antimutagenic activity, and antimicrobial evaluation against pathogenic microorganisms

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