The increase in malaria transmission in the Amazon region motivated vector control units of the Ministry of Health of Ecuador and Peru to investigate Anopheles (Diptera: Culicidae) species present in transmission hotspots. Mosquitoes were collected using prokopack aspirators and CDC light traps (Ecuador) and human landing catch in Peru. In Ecuador, 84 Anopheles were captured from Pastaza, Morona Santiago, and Orellana provinces and identified morphologically [An. (An.) apicimacula Dyar and Knab, An. (Nys.) near benarrochi, An. (Nys.) near oswaldoi, An. (Nys.) near strodei, An. (An.) nimbus (Theobald, 1902), and An. (Nyssorhynchus) sp.]. In Peru, 1,150 Anopheles were collected in Andoas District. A subsample of 166 specimens was stored under silica and identified as An. near oswaldoi, An. darlingi, and An. (An.) mattogrossensis Lutz and Neiva. COI barcode region sequences were obtained for 137 adults (107 from Peru, 30 from Ecuador) identified by ITS2 PCR-RFLP as An. benarrochi Gabaldon, Cova Garcia, and Lopez and retained in the final analysis. Haplotypes from the present study plus An. benarrochi B GenBank sequences grouped separately from Brazilian An. benarrochi GenBank sequences by 44 mutation steps, indicating that the present study specimens were An. benarrochi B. Our findings confirm the presence of An. benarrochi B in Ecuador and reported here for the first time from the Amazonian provinces of Orellana and Morona Santiago. Furthermore, we confirm that the species collected in Andoas District in the Datem del Maranon Province, Peru, is An. benarrochi B, and we observed that it is highly anthropophilic. Overall, the known distribution of An. benarrochi B has been extended and includes southern Colombia, much of Peru and eastern Ecuador.
La paleoparasitología en la Argentina ha producido un gran número de trabajos, especialmente en Patagonia. Sin embargo, hay ausencia de estos estudios en las poblaciones de la región central del país. En este sentido, realizamos la primera investigación sobre infestaciones parasitarias en restos humanos de sitios arqueológicos de la provincia de Córdoba. Se estudiaron 13 individuos de 4 sitios arqueológicos, datados entre 2562 ± 47 y 533 ± 57 14C años AP. Los análisis paleoparasitológicos se aplicaron sobre 20 muestras de sedimentos provenientes de la cavidad pélvica, empleando las técnicas de sedimentación espontánea, disgregación en ácido clorhídrico y flotación en sacarosa. Se hallaron estructuras sugestivas de huevos de helmintos de las superfamilias Ascaridoidea y Heterakoidea, y otros de nemátodos no identificados. Debido a que los animales son los huéspedes definitivos de estos parásitos, los resultados podrían sugerir la presencia de falso parasitismo en los individuos de la región. La relación cercana de los humanos con los animales, utilizados como parte de la dieta, se sustenta en estudios isotópicos y zooarqueológicos previos. La escasez de hallazgos parasitarios en este estudio posiblemente se debe a factores tafonómicos y a las características ambientales de los sitios.
Experimental paleoparasitological approaches have been used in order to optimize the methodology previously to the application in archeological samples. In this study we evaluated the action of dehydration and local soil (Central Argentina) on the loss of parasite eggs in experimental coprolites, using two parasitological techniques: spontaneous sedimentation and sucrose-flotation. Experimental coprolites comprised fresh human feces, positive for Hymenolepis nana, Ascaris sp., and Enterobius vermicularis, submitted to controlled artificial dehydration. Experimental coprolites with soil addition were prepared by mixing archeological sediment with equal mass of fresh feces. Helminth eggs were counted and eggs per gram were estimated in each subsample. Statistical analyses were applied to compare subsamples before and after desiccation and with and without addition of soil sediment. The performance of parasitological methods statistically differed, the sucrose flotation technique being the less effective when fresh feces and experimental coprolites were analyzed. Partial deformation of eggs was observed via both techniques only in subsamples containing H. nana eggs. However, this was not seen in Ascaris sp. subsamples, possibly due to eggshell composition. We found that sample desiccation significantly decreased the number of eggs in the experimental coprolites. Mixing archeological sediment with the fecal material also resulted in significantly fewer eggs surviving, independent of desiccation. This shows that climate and soil in which archeological fecal samples are found can strongly influence the survival of parasite eggs from past populations. The small amount of parasite evidence often found in paleoparasitological analyses, including Central Argentina, could be attributed to the action of taphonomic processes rather than to the real absence of infection in these ancient populations. Importantly, the study highlights the role of local soil, confirmed for the first time by empirical data. The research provides valuable insights into the understanding of the paleoparasitological results of the region and of general paleoparasitology.
We conducted a paleoparasitological study on sediment samples from two trash pits and a cesspool, collected during an archaeological assessment of a building located in the historic downtown of the city of Córdoba, Argentina. People have used these premises for residential and commercial purposes since the beginning of the seventeenth century, although the samples analyzed correspond to nineteenth-century contexts. Light microscopy examination revealed the presence of parasite eggs of whipworm (Trichuris sp.), possibly roundworm (Ascaris lumbricoides), and tapeworm (taeniid). The presence of these fecal-oral and food-borne transmitted helminths supports other lines of evidence that indicate poor sanitation and hygiene habits and inadequate food processing, which may have contributed to the high incidence and mortality of gastrointestinal diseases recorded at that time. The paleoparasitological data agree with the historical information on the health status of the populations that inhabited the city of Córdoba in the past, especially in relation to their habits and diet.
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