Higher temperatures lead to an increase of testicular metabolism that results in spermatic damage. Oxidative stress is the main factor responsible for testicular damage caused by heat stress. The aim of this study was to evaluate lasting effects of heat stress on ejaculated sperm and immediate or long-term effects of heat stress on epididymal sperm. We observed decrease in motility and mass motility of ejaculated sperm, as well as an increase in the percentages of sperm showing major and minor defects, damaged plasma and acrosome membranes, and a decrease in the percentage of sperm with high mitochondrial membrane potential in the treated group until one spermatic cycle. An increased enzymatic activity of glutathione peroxidase and an increase of stressed cells were observed in ejaculated sperm of the treated group. A decrease in the percentage of epididymal sperm with high mitochondrial membrane potential was observed in the treated group. However, when comparing immediate and long-term effects, we observed an increase in the percentage of sperm with low mitochondrial membrane potential. In conclusion, testicular heat stress induced oxidative stress that led to rescuable alterations after one spermatic cycle in ejaculated sperm and also after 30 days in epididymal sperm.

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Our study aimed to assess the impact of sperm oxidative stress on embryo development by means of a dose-dependent model. In experiment 1, straws from five bulls were subjected to incubation with increasing H2O2 doses (0, 12.5, 25, and 50 μM). Motility parameters were evaluated by Computed Assisted System Analysis (CASA). Experiment 2 was designed to study a high (50 μM) and low dose (12.5 μM) of H2O2 compared to a control (0 μM). Samples were incubated and further used for in vitro fertilization. Analyses of motility (CASA), oxidative status (CellROX green and 2'-7' dichlorofluorescein diacetate), mitochondrial potential (JC-1), chromatin integrity (AO), and sperm capacitation status (chlortetracycline) were performed. Embryos were evaluated based on fast cleavage (30 h.p.i.), cleavage (D = 3), development (D = 5), and blastocyst rates (D = 8). We observed a dose-dependent deleterious effect of H2O2 on motility and increase on the percentages of positive cells for CellROX green, capacitated sperm, and AO. A decrease on cleavage and blastocyst rates was observed as H2O2 increased. Also, we detected a blockage on embryo development. We concluded that sperm when exposed to oxidative environment presents impaired motility traits, prooxidative status, and premature capacitation; such alterations resulting in embryo development fail.

Road detection is a crucial task in autonomous navigation systems. It is responsible for delimiting the road area and hence the free and valid space for maneuvers. In this paper, we consider the visual road detection problem where, given an image, the objective is to classify every of its pixels into road or non-road. We address this task by proposing a convolutional neural network architecture. We are especially interested in a model that takes advantage of a large contextual window while maintaining a fast inference. We achieve this by using a Network-in-Network (NiN) architecture and by converting the model into a fully convolutional network after training. Experiments have been conducted to evaluate the effects of different contextual window sizes (the amount of contextual information) and also to evaluate the NiN aspect of the proposed architecture. Finally, we evaluated our approach using the KITTI road detection benchmark achieving results in line with other state-of-the-art methods while maintaining real-time inference. The benchmark results also reveal that the inference time of our approach is unique at this level of accuracy, being two orders of magnitude faster than other methods with similar performance.

Resumo -O objetivo deste trabalho foi avaliar a influência de grãos de soja (GS) no desempenho, características e rendimento de cortes de carcaça de cordeiros confinados. Sessenta e quatro cordeiros Santa Inês, com peso vivo inicial de 19,5±0,19 kg e 75±2 dias de idade, foram distribuídos em blocos ao acaso de acordo com o peso vivo e a idade no início do experimento. Os GS participaram com 0, 7, 14 e 21% na matéria seca (MS) das rações isonitrogenadas experimentais. O consumo de MS (1,1, 1,0, 0,9 e 0,9 kg por dia), proteína bruta (199,2, 181,5, 179,0 e 175,2 g por dia) e o ganho de peso vivo (298, 275, 280 e 255 g por dia) diminuíram linearmente, e o consumo de extrato etéreo (43,8, 49,7, 57,2 e 66,0 g por dia) aumentou linearmente com o aumento da participação de GS nas rações. Não houve efeito na conversão alimentar, características e rendimento de cortes da carcaça. A inclusão de GS na ração com alto teor de concentrado proporciona desempenho satisfatório aos cordeiros em confinamento.Termos para indexação: confinamento, gordura, Santa Inês, ovino. Performance and carcass characteristics of feedlot lambs fed raw soybeanAbstract -The objective of this work was to evaluate the effects of raw soybean on lamb performance, carcass yield and lamb cuts. Raw soybean was added at 0, 7, 14 and 21% of the diet dry matter (isonitrogenous). Sixtyfour Santa Inês lambs (initial body weight 19.5±0.19 kg and 75±2 days old) were assigned to a complete randomized block design according to body weight and age at beginning of the trial. Dry matter intake (1.1, 1.0, 0.9 and 0.9 kg per day), crude protein intake (199.2, 181.5, 179.0 and 175.2 g per day) and average daily gain (298, 275, 280, 255 g per day) decreased linearly, and ether extract intake (43.8, 49.7, 57.2 and 66.0 g per day) increased linearly, as levels of raw soybean increased. Feed conversion, carcass yield and lamb cuts were not affected by raw soybean inclusion in the diet. The inclusion of raw soybean in high concentrate diets provides satisfactory feedlot lamb performance.Index terms: feedlot, fat, Santa Inês, sheep. IntroduçãoO rebanho nacional de ovinos é de aproximadamente 15 milhões de cabeças, representando 1,4% do efetivo mundial, e concentra-se nas regiões Sul (30%) e Nordeste (58%) (Brasil, 2006). A produção de carne é uma atividade alternativa, capaz de aumentar a renda nos negócios, não só de ovinocultores, mas de toda a atividade rural.Apesar de a população brasileira não ter o hábito de consumir carne ovina, observa-se aumento do consumo nos últimos anos. O consumo per capita de carnes no Brasil é da ordem de 65 kg por ano; o consumo de carne de ovinos é de 0,7 kg por ano (Couto, 2001), valor considerado subestimado, já que a maior parte do comércio de carne ovina é informal.Ao se considerar o elevado custo da terra, principalmente na Região Sudeste, o confinamento é uma estratégia capaz de satisfazer tanto o produtor quanto o consumidor, uma vez que permite reduzir o ciclo de produção e disponibilizar ao mercado carcaças de animais jovens e, conse...

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Background: The aim of this study was to evaluate the distribution of sheep breeds in Brazil, correlate their occurrence with environmental factors and determine their risk for extinction.