Sperm Oxidative Stress Is Detrimental to Embryo Development: A Dose-Dependent Study Model and a New and More Sensitive Oxidative Status Evaluation

Castro, Luiz Guilherme Martins; Assis, Patrícia Monken de; Siqueira, Adriano Felipe Perez; Hamilton, Thais Rose dos Santos; Mendes, Clayton Quirino; Losano, João Diego de Agostini; Nichi, Marcílio; Visintin, José Antônio; Assumpção, Mayra Elena Ortiz D’Ávila

doi:10.1155/2016/8213071

Cited by 75 publications

(55 citation statements)

References 62 publications

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“…For example, sperm that suffer from oxidative stress may incur irreparable physical and chemical modifications, such as lipid peroxidation and antioxidant depletion, which can damage embryonic development (de Castro et al, ). Increased exposure to oxidative stress was reported to compromise sperm DNA integrity, reduce cleavage rate, and increase the number of apoptotic cells in embryos that reached the blastocyst stage (Simoes et al, ; de Castro et al, ). In our experiments, the 10 −3 M melatonin‐dependent improvement in semen characteristics ultimately enhanced development to the blastocyst stage and reduced the number of apoptotic embryonic cells.…”

Section: Discussionmentioning

confidence: 99%

Protective effects of melatonin on bovine sperm characteristics and subsequent in vitro embryo development

Pang

Sun

Jiang

et al. 2016

Molecular Reproduction Devel

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We aimed to investigate the effect of melatonin on bovine frozen-thawed semen and its impact on fertilization outcome. Plasma membrane integrity, mitochondrial activity, acrosome integrity, and levels of intracellular reactive oxygen species (ROS) were measured in spermatozoa treated with different concentrations of melatonin. Melatonin-treated spermatozoa were then used for in vitro fertilization, followed by analysis of subsequent embryo development and the expression of apoptosis- and antioxidant-related genes. The results revealed that 10 and 10 M melatonin led to higher plasma membrane integrity, mitochondrial activity, and acrosome integrity, and significantly decreased intracellular ROS levels (P < 0.05). The blastocyst development rate of in vitro-produced bovine embryos originating from 10 M melatonin-treated spermatozoa was significantly higher, while the incidence of apoptotic nuclei in blastocysts was markedly lower than for embryos from any other group (P < 0.05). CASP3 and BAX mRNA abundance were significantly reduced whereas BCL2, XIAP, and CAT transcript abundance were significantly increased in embryos produced from spermatozoa treated with 10 M melatonin; GPX4 expression, however, was comparable in all treatment groups. Thus, 10 M melatonin can improve the quality of bovine frozen-thawed semen. These beneficial effects appear to influence preimplantation embryos, given the correlation with its anti-apoptotic and anti-oxidative properties. Mol. Reprod. Dev. 83: 993-1002, 2016 © 2016 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 99%

Protective effects of melatonin on bovine sperm characteristics and subsequent in vitro embryo development

Pang

Sun

Jiang

et al. 2016

Molecular Reproduction Devel

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“…Reduction of motility, viability, and mitochondrial potential and increased membrane and DNA peroxidative damage are expected characteristics in cryopreserved semen (Gürler et al, 2016;Castro et al, 2015). This is because thawed semen loses significant amounts of SOD and GPx and has increased levels of superoxide anion and hydrogen peroxide (Bilodeau et al, 2000;Chatterjee and Gagnon, 2001).…”

Section: Tablementioning

confidence: 99%

Does lipid peroxidation and oxidative DNA damage differ in cryopreserved semen samples from young, adult and aged Nellore bulls?

Trevizan

Carreira

Carvalho

et al. 2018

Animal Reproduction Science

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The aims of this study were to evaluate cryopreserved semen of Nellore bulls of different ages and verify whether sperm quality declines with advancing age and whether lipid peroxidation and DNA damage are involved in this process. For this purpose, 40 Nellore bulls were divided into three age groups: Young, aged 1.8-2 years (n = 9); Adult, aged 3.5-7.0 years (n = 19); and Seniors, aged 8.0-14.3 years (n = 12). Three ejaculates were collected from each bull, cryopreserved and evaluated for various parameters including membrane integrity, mitochondrial potential (FITC-PSA and JC1), lipid peroxidation (C-11BODIPY 581 / 591) and oxidative DNA damage (8OHdG) using flow cytometry. The thawed semen of senior bulls was characterized by a low percentage of motile sperm (33.7 ± 6.1%), higher damage to the plasma and acrosomal membrane (37.5 ± 9.8%), and low mitochondrial potential (29.1 ± 13.8%), as well as higher percentages of peroxidated cells (53.6 ± 12.2%) and DNA damage (44.1 ± 11.0%; P < 0.05). Lipid peroxidation was negatively correlated with motility (r = -0.35, P < 0.0002), average mitochondrial potential (r = -0.42; P < 0.0001) and showed a positive correlation with membrane injury and oxidative DNA damage (r = 039; P = 0.0003). Young bulls presented superior thawed sperm quality, possibly due to greater resistance to oxidative stress and, consequently, to cryopreservation. In conclusion, the sperm quality of bull semen declines with advancing age and is strongly associated with increased oxidative damage to both the plasma membrane and DNA.

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“…There are many reports studied the relationship between capacitation and embryo development, such as bull sperm which submitted to an oxidative environment negatively influence embryo development when used for in vitro fertilization [156]. Also, DNA damage in the form of strand breaks and base adduct formation is another common feature of defective spermatozoa which may be directly correlated with the normality of early embryonic development [275–276].…”

Section: Capacitation Related To Assisted Reproductive Technology Andmentioning

confidence: 99%

Factors and pathways involved in capacitation: how are they regulated?

Jin

Yang

2016

Oncotarget

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In mammals, fertilization occurs via a comprehensive progression of events. Freshly ejaculated sperm have yet to acquire progressive motility or fertilization ability. They must first undergo a series of biochemical and physiological changes, collectively known as capacitation. Capacitation is a significant prerequisite to fertilization. During the process of capacitation, changes in membrane properties, intracellular ion concentration and the activities of enzymes, together with other protein modifications, induce multiple signaling events and pathways in defined media in vitro or in the female reproductive tract in vivo. These, in turn, stimulate the acrosome reaction and prepare spermatozoa for penetration of the egg zona pellucida prior to fertilization. In the present review, we conclude all mainstream factors and pathways regulate capacitation and highlight their crosstalk. We also summarize the relationship between capacitation and assisted reproductive technology or human disease. In the end, we sum up the open questions and future avenues in this field.

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Sperm Oxidative Stress Is Detrimental to Embryo Development: A Dose-Dependent Study Model and a New and More Sensitive Oxidative Status Evaluation

Cited by 75 publications

References 62 publications

Protective effects of melatonin on bovine sperm characteristics and subsequent in vitro embryo development

Protective effects of melatonin on bovine sperm characteristics and subsequent in vitro embryo development

Does lipid peroxidation and oxidative DNA damage differ in cryopreserved semen samples from young, adult and aged Nellore bulls?

Factors and pathways involved in capacitation: how are they regulated?

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