No abstract

No abstract

No abstract

No abstract

We complement the MALATANG sample of dense gas in nearby galaxies with archival observations of 12CO and its isotopologues to determine scaling relations between Wide-field Infrared Survey Explorer (WISE) 12 μm emission and molecular gas tracers at subkiloparsec scales. We find that 12 μm luminosity is more tightly correlated with 12CO than it is with 13CO or dense gas tracers. Residuals between predicted and observed 12CO are only weakly correlated with molecular gas mass surface density (Σmol) in regions where Σmol is very low (∼10 M ⊙ pc−2). Above this limit, the 12CO residuals show no correlations with physical conditions of molecular gas, while 13CO residuals depend on the gas optical depth and temperature. By analyzing differences from galaxy to galaxy, we confirm that the 12CO−12 μm relation is strong and statistically robust with respect to star-forming galaxies and active galactic nucleus hosts. These results suggest that WISE 12 μm emission can be used to trace total molecular gas instead of dense molecular gas, likely because polycyclic aromatic hydrocarbons (PAHs, a major contributor to WISE 12 μm emission) may be well mixed with the gas that is traced by 12CO. We propose that WISE 12 μm luminosity can be used to estimate molecular gas surface density for statistical analyses of the star formation process in galaxies.

Background: Hemorrhoids are a frequently-occurring disease of the anorectal system that is often accompanied by vascular hyperplasia and edema. A METTL14-mediated RNA N-6 methyladenosine (m6A) modification can improve mRNA stability and increase its transcriptional and translational activities, closely related to the occurrence of many diseases.Methods: Western blot, qPCR, and immunofluorescence staining were used to detect the levels of gene and protein expression. Haematoxylin and eosin staining was used for histopathological examination. RNA immunoprecipitation-PCR and RNA dot blotting were used to detect mRNA m6A modification.Results: Obvious signs of angiogenesis (CD31+/vWF+) were identified in the hemorrhoids. High levels of METTL14 expression on vascular endothelial cells (CD31+) suggested that angiogenesis was accompanied by differential modification of m6A RNA. It was subsequently found that the level of miR-4729 expression was significantly decreased in hemorrhoid tissues. The luciferase reporter enzyme assay results suggested that miR-4729 silenced its expression by targeting the 3'UTR of METTL14 mRNA. MiR-4729 overexpression in human umbilical vein endothelial cells (HUVECs) inhibited the proliferation and migration of HUVECs in vitro and vascular structure formation in the outer matrix. MiR-4729 overexpression significantly inhibited endogenous METTL14 expression in HUVECs and reduced the entire m6A RNA modification, especially the level of m6A methylation at the specific site of the 3' UTR of TIE1 mRNA. Moreover, miR-4729 overexpression significantly inhibited the molecular loop of the TIE1/VEGFA signaling pathway in HUVECs.Conclusions: Our findings confirmed that the down-regulation of miR-4729 in hemorrhoid vascular endothelial cells was one of the main reasons for vascular proliferation. The overexpression of miR-4729 in vascular endothelial cells decreased the global mRNA methylation and TIE1 mRNA 3'UTR-specific site methylation by silencing METTL14 expression, reducing TIE1 mRNA stability, down-regulating the TIE1/ VEGFA signal molecular loop expression, and weakening angiogenesis ability.

No abstract

Currently researchers expect to control the internal noise of an enclosure by controlling the relative structural vibration. The method is called ANC (Active Noise Control). Sound pressure response and velocity response of the structure are two significant factors to evaluate the performance of the ANC. But previous researches have to depend on a three-dimensional model with regular shape. Based on the Green's function theorem this paper proposed two formulas to describe the contributions of acoustic modes and structural modes. These two formulas are the essential basis for the analysis of structural-acoustic coupling of an enclosure. From them compact matrix formulas are deduced and a numerical simulation method is presented to calculate the responses of sound pressure and velocity. The numerical simulation method is verified by comparing the numerical result with the theoretical one based on a regular model. And the application of the method in an irregular model shows this method can be used to analyze any model with arbitrary shape.