miR-412-5p targets Xpo1 to regulate angiogenesis in hemorrhoid tissue

Wang, Chen; Lü, Hong; Luo, Chao; Song, Chengkun; Wang, Qing-Ming; Peng, Yunhua; Yao-jie, Xin; Liu, Te; Yang, Wei

doi:10.1016/j.gene.2019.04.058

Cited by 15 publications

(12 citation statements)

References 33 publications

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“…The musk is warm in nature, has the functions of promoting blood circulation and reducing swelling, clearing the meridians and expelling knots and relieving pain. Bezoar is a commonly used medicine for clearing away heat and detoxification, and its efficacy in the treatment of carbuncle, gangrene, boils, sores, and other diseases has been certified; borneol has the functions of clearing heat and relieving pain, antiseptic, and muscle regeneration and has anti-inflammatory, analgesic, and antibacterial functions; pearls contain an appropriate amount of calcium carbonate and various amino acids and has a pivotal role in clearing heat and detoxifying, promoting muscle and astringing sores, hemostasis, and anti-inflammatory [ 16 , 17 ].…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of Mayinglong Musk Hemorrhoid Ointment on Wound Healing and Complications after Internal Hemorrhoid Ligation and External Hemorrhoidectomy

Lin

Zang

2022

Evidence-Based Complementary and Alternative Medicine

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Objective. To investigate the effect of Mayinglong Musk Hemorrhoids Ointment on wound healing and complications after internal hemorrhoid ligation and external hemorrhoidectomy. Methods. This is a retrospective study. A total of 100 patients with mixed hemorrhoids who were treated in our hospital from August 2019 to October 2020 were recruited and assigned to an operation group (n = 50, internal hemorrhoid ligation, and external hemorrhoidectomy) or a combined group (n = 50, use of Mayinglong Musk Hemorrhoid Ointment after internal hemorrhoid ligation and external hemorrhoidectomy). The wound-healing effect, wound-healing time, visual analog scale (VAS), anal function, and complications were compared between the two groups. Results. The combined group exhibited a significantly higher total effective rate of wound healing when compared with the operation group ( p < 0.05 ). The patients in the combined group experienced remarkably faster wound healing than the operation group ( p < 0.05 ). The visual analog scale (VAS) score of the combined group was significantly lower than that of the operation group ( p < 0.05 ). The combined group obtained superior anal function than the operation group ( p < 0.001 ). Conclusion. Mayinglong Musk Hemorrhoid Ointment combined with internal hemorrhoid ligation and external hemorrhoidectomy in the treatment of mixed hemorrhoids yields remarkable outcomes. It improves the wound healing outcomes, accelerates wound healing, relieves postoperative pain, enhances anal function, and reduces the occurrence of postoperative complications in the patient.

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Section: Discussionmentioning

confidence: 99%

Effectiveness of Mayinglong Musk Hemorrhoid Ointment on Wound Healing and Complications after Internal Hemorrhoid Ligation and External Hemorrhoidectomy

Lin

Zang

2022

Evidence-Based Complementary and Alternative Medicine

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“…26 Using quantitative approaches to analyze exosomal miRNA contents, we further demonstrated that the exosomes derived from OGD-treated bEnd.3 cells were clearly distinguishable from the exosomes isolated from control cells. We further analyzed the eight miRNAs that were most significantly affected by OGD treatment and found that these miRNAs regulate a variety of functions, including cell proliferation and migration (miRNA-122-5p and miR-409-3p), 27 vascular structure formation (miR-412-5p), 28 nerve fiber regeneration (miR-379-5p), 29 cell apoptosis (miR-494-3p), 30 and cell adhesion (miR-127-3p). 31 The exosomes from different sources can be distinguished by identifying their surface protein combinations.…”

Section: Discussionmentioning

confidence: 99%

Combined Analysis of Surface Protein Profile and microRNA Expression Profile of Exosomes Derived from Brain Microvascular Endothelial Cells in Early Cerebral Ischemia

Yang

Gao

et al. 2021

ACS Omega

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Endothelial cell damage is an important pathological basis for the deterioration of acute ischemia stroke. Our previous studies have been exploring the mechanism of blood–brain barrier (BBB) endothelial cell injury in the early stage of cerebral ischemia. Exosomes act as an important intercellular player in neurovascular communication. However, the characteristic of exosomes derived from BBB endothelial cells in early ischemic stroke is poorly understood. We exposed cultured brain microvascular endothelial cells (bEnd.3) to 3 h oxygen glucose deprivation (OGD) to mimic early cerebral ischemia in vitro and compared miRome and surface protein contents of exosomes derived from bEnd.3 cells by miRNA sequencing and the proximity barcoding assay (PBA). A total of 346 differentially miRNA (159 upregulated and 187 downregulated) were identified via miRNA-Seq in bEnd.3 cells after exposure to OGD for 3 h. Moreover, Gene Ontology (GO) and KEGG pathway analyses showed that cell proliferation- and angiogenesis-associated miRNAs were significantly affected. The abnormal changes in top eight miRNAs were further verified by a quantitative polymerase chain reaction (qPCR). PBA experiments showed that the numbers of exosomes carrying the following proteins increased significantly under ischemia, including bFGF, CD146, EPHA2, ABCB5, and ITGB2. These proteins were related to angiogenesis, cell proliferation, and cell inflammation. The network analysis combining PBA data with miRNA-Seq data showed that 79 miRNAs were related to 24 membrane proteins and predicted that there were surface proteins associated with a variety of miRNA molecules, such as ITGA9, XIAP, ADAM1, ITGA2, ITGA3, PDPN, and ITGB1. Meanwhile, there were miRNAs related to various surface proteins including miR-410-3p, miR-378b, and miR-1960. Taken together, our data demonstrated for the first time the changes of exosomal miRNAs and surface protein profiles derived from ischemic microvascular endothelial cells, which may provide new therapeutic targets for BBB protection in ischemic stroke.

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“…The LIPE gene was potentially regulated by the XR_001041923.2-miR-412-5p relationship pair in this study. There were few studies on the lipid metabolism of miR-412-5p; however, previous studies suggested that the inactivation of miR-412-5p in vascular endothelial cells lead to the high expression of Xpo1 and the inhibition of the p53-p66SHC-p16 pathway, which ultimately promoted the formation of vascular endothelial cells and hemorrhoid blood vessels ( 58 ). These genes were involved in fatty acid and lipid metabolism during the growth and development of Tibetan sheep.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Transcriptome Analysis Reveals the Role of lncRNA in Fatty Acid Metabolism in the Longissimus Thoracis Muscle of Tibetan Sheep at Different Ages

Bao

Zhao

et al. 2022

Front. Nutr.

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Long noncoding RNA (lncRNA) plays an important regulatory role in mammalian adipogenesis and lipid metabolism. However, their function in the longissimus thoracis (LT) muscle of fatty acid metabolism of Tibetan sheep remains undefined. In this study, fatty acid and fat content in LT muscle of Tibetan sheep were determined, and RNA sequencing was performed to reveal the temporal regularity of lncRNA expression and the effect of lncRNA-miRNA-mRNA ceRNA regulatory network on lipid metabolism of LT muscle in Tibetan sheep at four growth stages (4-month-old, 4 m; 1.5-year-old, 1.5 y; 3.5-year-old, 3.5 y; 6-year-old, 6 y). The results indicated that the intramuscular fat (IMF) content was highest at 1.5 y. Moreover, the monounsaturated fatty acid (MUFA) content in 1.5 y of Tibetan sheep is significantly higher than those of the other groups (P < 0.05), and it was also rich in a variety of polyunsaturated fatty acids (PUFA). A total of 360 differentially expressed lncRNAs (DE lncRNAs) were identified from contiguous period transcriptome comparative groups of 4 m vs. 1.5 y, 1.5 y vs. 3.5 y, 3.5 y vs. 6 y, and 4 m vs. 6 y, respectively. Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis found that the target genes in lncRNA trans-mRNA were significantly related to the protein digestion, absorption, and fatty acid biosynthesis pathways (P < 0.05), which demonstrated that DE lncRNA trans-regulated the target genes, and further regulated the growth and development of the LT muscle and intramuscular fatty acid metabolism in Tibetan sheep. We further analyzed the role of the lncRNA-miRNA-mRNA regulatory network in the lipid metabolism of Tibetan sheep. Additionally, GPD2, LIPE (lipase E hormone-sensitive enzyme), TFDP2, CPT1A, ACACB, ADIPOQ, and other mRNA related to fatty acid and lipid metabolism and the corresponding lncRNA-miRNA regulatory pairs were identified. The enrichment analysis of mRNA in the regulatory network found that the AMPK signaling pathway was the most significantly enriched (P = 0.0000112361). Comprehensive transcriptome analysis found that the LIPE, ADIPOQ, ACACB, and CPT1A that were regulated by lncRNA might change the formation of energy metabolism in Tibetan sheep muscle through the AMPK signaling pathway, and oxidized muscle fibers are transformed into glycolytic muscle fibers, reduced IMF content, and the fatty acid profile also changed.

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miR-412-5p targets Xpo1 to regulate angiogenesis in hemorrhoid tissue

Cited by 15 publications

References 33 publications

Effectiveness of Mayinglong Musk Hemorrhoid Ointment on Wound Healing and Complications after Internal Hemorrhoid Ligation and External Hemorrhoidectomy

Effectiveness of Mayinglong Musk Hemorrhoid Ointment on Wound Healing and Complications after Internal Hemorrhoid Ligation and External Hemorrhoidectomy

Combined Analysis of Surface Protein Profile and microRNA Expression Profile of Exosomes Derived from Brain Microvascular Endothelial Cells in Early Cerebral Ischemia

Comprehensive Transcriptome Analysis Reveals the Role of lncRNA in Fatty Acid Metabolism in the Longissimus Thoracis Muscle of Tibetan Sheep at Different Ages

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