RESUMO:Este artigo é uma revisão bibliográfica sobre as espécies brasileiras de Passiflora (Passiflora edulis fo. flavicarpa O. Deg., P. alata Curtis e P. edulis fo. edulis). A maioria dos artigos da literatura focaliza somente as folhas de Passiflora, enquanto que esta revisão contém informações sobre a polpa, cascas e sementes dos frutos do maracujá, com destaque para a composição química, estudos nutricionais e farmacológicos. O enfoque nos frutos do maracujá fundamenta-se no amplo consumo do suco de maracujá (fresco ou industrializado) no Brasil e também nas investigações em andamento para avaliar o seu potencial uso como alimento funcional.Unitermos: Passiflora, composição química, alimento funcional.ABSTRACT: "Passion fruit: a functional food?" This paper consists of a bibliographic review of the most relevant edible Brazilian Passiflora species (Passiflora edulis fo. flavicarpa O. Deg., P. alata Curtis and P. edulis fo. edulis). Most of the reports in the literature focus solely on Passiflora leaves, whereas this review contains information about passion fruit pulp, rind and seeds, highlightening chemical composition, nutritional and pharmacological studies. The emphasis on the "maracujá" fruit is due to the extensive consumption of passion fruit juice (fresh or processed) in Brazil and on ongoing investigations into its potential as a functional food.
HPLC-MS using collision induced dissociation (CID) has been utilised for the identification of the C-glycosylflavone isomer pairs orientin/isoorientin and vitexin/isovitexin. HPLC-CID/MS analyses produced pseudo-MS/MS spectra that allowed the identification of the flavone C-glycosides. The efficient differentiation of isomers was performed by comparing the CID-MS/MS spectra (including exact mass measurements) of particular fragments from the C-glycoside unit. In order to illustrate some possibilities of these MS techniques, they were applied to the comparative analyses of extracts of Passiflora alata, P. edulis, P. incarnata and P. caerulea (Passifloraceae) that are employed as phytomedicines in Brazil and South America.
RESUMO Objetivo: analisar as disciplinas de Enfermagem Psiquiátrica e Saúde Mental nas estruturas curriculares dos cursos de graduação em enfermagem brasileiros. Método: estudo exploratório de análise documental, cujos dados foram coletados nos websites das instituições que possuíam cadastro no site do Instituto Nacional de Estudos e Pesquisas Educacionais Anísio Teixeira, através de instrumento elaborado para esse estudo, com informações relacionadas à instituição, disciplinas oferecidas na área, localização da(s) disciplina(s) no currículo e carga horária. Os dados obtidos foram lançados no Statistical Package for the Social Sciences 2.0 para realização da estatística descritiva. Resultados: dos 738 cursos cadastrados, 88,8% são oferecidos por instituições privadas, 72% das instituições pesquisadas disponibilizavam matriz curricular do curso on-line, dentre essas, 47,2% apresentavam ao menos uma disciplina na área, com média de 96 horas nas instituições privadas e 142 horas nas públicas, perfazendo 2,4% e 3,5% da carga horária total do curso, respectivamente. Conclusão: verificou-se a predominância de instituições privadas ofertando graduação em enfermagem e que há cursos que não oferece disciplina relacionada à Enfermagem Psiquiátrica e Saúde Mental. Notou-se também que não houve uma padronização de nomenclatura e do período para oferecimento dessa disciplina. Sugere-se pesquisas na área sobre o modo de como são ministrados os conteúdos teóricos e prático dessa disciplina.
A high-performance thin layer chromatographic (HPTLC) method was developed in order to determine quantitatively the flavonoids in leaves of Passiflora alata, P. edulis, P. caerulea and P. incarnata. The content of orientin and isoorientin was determined, and the results were compared with those obtained using a quantitative HPLC-UV method. The latter employed rutin as standard and was developed to analyse flavonoid content from Passiflora leaves for the purpose of ensuring the quality of Passiflora phytomedicines. The results obtained using the two methods indicate that there are qualitative and quantitative differences in the flavonoids of the reference Passiflora species studied. The two methods were also employed to analyse commercial samples to illustrate their application in qualitative ('fingerprint') and quantitative determination, demonstrating their feasibility in the quality control of flavonoids from crude Passiflora drugs and phytomedicines. The HPLC conditions used are also suitable for the quantitative analysis of aqueous extracts (Passiflora infusions).
Introduction Plant medicine/herbal extracts are typically complex, encompassing a wide range of flavonoid diversity and biological benefits. Combined with a lack of standards; species authentication profiling is a challenge. A non‐targeted screening strategy using two‐dimensional (2D) separation and specificity of ultra‐high‐performance liquid chromatography ion mobility collision‐induced dissociation mass spectrometry (UHPLC‐IM‐CID‐MS) has been investigated, to identify the 6‐C and 8‐C‐glycosylflavone isomer orientin/isoorientin and vitexin/isovitexin pairs in Passiflora species. Utilising available standards and “known–unknowns” a reference CCS (collision cross‐section) speciation finger print for Passiflora extracts could be generated to illustrate species profiling. Material and Methods SPE was performed to extract flavonoids of interest from powdered and ground Passiflora leaf. Chromatographic separation was achieved via UHPLC and analysis performed using positive/negative ion electrospray coupled with linear T‐wave IM‐MS (calibrated to perform accurate mass and CCS measurements). Results Comparative phytochemical screening of Passiflora alata, P. edulis, P. incarnata and P. caerulea leaf extracts has generated CCS, CID IM product ion spectra, 2D separation with UHPLC‐IM‐MS, enabling the unequivocal identification of flavone C‐glycosides in complex extracts. A phytochemical reference CCS library was generated comprised of “knowns” and “known–unknowns”. Isomers have been differentiated using a CCS metric enabling novel CCS specific isomeric quantitation of co‐eluting isomers. Conclusions The screening approach illustrated has the potential to play an important role in the profiling of medicinal plants to determine phytochemical make‐up and improve consumer safety through generation of highly specific speciation profiles.
Objective: to determine the effect of sociodemographic and vulnerability variables on the alcohol use of university women.Method: it is an exploratory study composed for 301 college women. Sociodemographic and vulnerability data were obtained through electronic questionnaire (CAGE, AUDIT-C, sociodemographic and vulnerability to alcohol questionnaire). The data analyze, were used the chi-square test and logistic regression.Result: this results showed that 80.4% of women showed moderate use and 19.6% showed harmful use of alcohol. The variables that showed effect in use pattern were: age of first use, socioeconomic level, kind of drink, and social network. Conclusions:we can conclude that the vulnerability factors, destiled alcohol drinks, social networks that promote beverage consumer, and onset the use between 14 and 16 years are characteristics that can promote the nocive comsumption. DESCRIPTORS:Women. Alcohol drinking. Vulnerability. Young adult. Student. EFEITO DAS VARIÁVEIS SOCIODEMOGRÁFICAS E DE VULNERABILIDADE NO PADRÃO DE USO DE ÁLCOOL EM MULHERES UNIVERSITÁRIAS RESUMOObjetivo: determinar o efeito das variáveis sociodemográficas e de vulnerabilidade no uso de bebidas alcoólicas em mulheres universitárias.Método: estudo transversal, composto por uma amostra de 301 mulheres universitárias. Os dados sociodemográficos e de vulnerabilidade ao consumo foram obtidos pelos formulários eletrônicos CAGE, AUDIT-C, questionário sociodemográfico e de vulnerabilidade ao uso de álcool. Para análise dos dados foram usados o teste qui-quadrado e a regressão logística.Resultados: 80,4% das mulheres apresentaram uso moderado e 19,6 %, uso nocivo de álcool. As variáveis que mostraram ter efeito sobre o padrão de uso foram: idade de início do consumo, nível socioeconômico, tipo de bebida e redes sociais. Conclusão EFECTO DE LAS VARIABLES SOCIODEMOGRÁFICAS Y DE VULNERABILIDAD EN EL PATRÓN DE USO DE ALCOHOL EN MUJERES UNIVERSITARIAS RESUMENObjetivo: determinar el efecto de las variables sociodemográficas y de vulnerabilidad en el uso de bebidas alcohólicas en mujeres universitarias.Métodos: investigación transversal, compuesto por una muestra de 301 mujeres universitarias. Los datos sociodemográficos y de vulnerabilidad al consumo fueron obtenidos por los formularios electrónicos (CAGE, AUDIT-C), cuestionario sociodemográfico y de vulnerabilidad al uso del alcohol. Para análisis de los datos fueron usados el teste chi cuadrado y la regresión logística.Resultados: 80,4% de las mujeres presentaron el uso moderado y 19,6%, uso nocivo de alcohol. Las variables que mostraron tener efecto sobre el patrón de uso fueron: edad de inicio del consumo, nivel socioeconómico, tipo de bebida y redes sociales.Conclusión: los factores de vulnerabilidad, preferencia por bebidas alcohólicas destiladas, tener redes sociales que promuevan el consumo, haber iniciado el uso entre 14 y 16 anos de edad son características que pueden favorecer el consumo nocivo de alcohol. DESCRIPTORES:Mujeres. Consumo de bebidas alcohólicas. Vulnerabilidad. A...
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