Pigs are the main host species for the pseudorabies virus, while it causes fatal encephalitis in many species including human being. The objective of this article is to report the rst clinical case of pseudorabies as well as isolation and molecular characterization of the virus from a hunting dog in Bursa province, Turkey. The dog showing clinical signs including pruritus and neurological signs as stumbling and inability to stand up compatible with pseudorabies. The virus isolate was obtained from supernatant of fresh tissue samples from cerebellum, cornu ammonis, spleen, salivary gland, and conjunctival swab, as well as serum, and buffy coat samples. The glycoprotein C region is targeted for viral DNA ampli cation. Pseudorabies virus genome was detected both in fresh tissues and supernatants of 3 rd passage on Vero cells. Number of PCR positive samples were dramatically increased after having cell culture inoculations. Genome sequencing revealed that the strain (Bursa-10303) isolated in Turkey where a non-endemic area is for the virus is dropped into clade A. This study con rms the presence of psedorabies infection in the wild life reservoirs in Turkey. Future studies may clarify the importance of the infection for Turkey where there is no common pig production.
Sığır solunum sistemi hastalıkları kompleksi (BRDC) başta sığırlar olmak üzere birçok çift tırnaklı hayvan türünü etkileyen ve ülkemiz dahil olmak üzere tüm dünyada yaygın olarak görülen bir problemdir. Bu çalışmada Diyarbakır ilini temsil edecek şekilde 8 farklı ilçeden 229 sığır, 192 koyun ve 12 keçi olmak üzere toplam 433 serum örneğinde Bovine viral diarrhoea virus (BVDV), Bovine herpesvirus-1 (BoHV-1), Bovine respiratory syncytial virus (BRSV), Bovine coronavirus (BCoV) ve Bovine parainfluenza-3 (BPIV-3) viruslarına karşı gelişmiş antikorların varlığı incelenmiştir. Virus nötralizasyon yöntemiyle belirlenen seroprevalans değerleri BVDV,
This study reports the high prevalence and molecular characterization of BoHV-1 infection in imported cattle with respiratory system disease after international transport. A high mortality rate of 14.16% (51/360) was reported in a group of animals imported from Hungary to Turkey in 2019. A total of 17 samples were evaluated (3 lung tissue and 14 nasal swab samples) from 15 cattle aged 6 to 9 months not vaccinated against BoHV-1. Virus isolation, polymerase chain reaction (PCR) and restriction endonuclease analysis (REA) procedures were performed within the scope of this study. By virus isolation in MDBK cells, cytopathologic effects was detected in 8 samples (3 lung tissue and 5 nasal swabs samples). The same eight samples were also found positive by BoHV-1 PCR targeting gC (UL44) gene region. According to the sequencing result, the sample (ID: 10054) dropped into a cluster of BoHV-1.1. The REA was applied to the samples to confirm the results of phylogenetic analysis. All of the isolates were identified in the subgroup BoHV-1.1 by REA. These results showed a high mortality risk for imported animals and the possibility for BoHV-1 entering the receiving country via imported animals after transport. This event is a serious problem both for the control of BoHV-1 as well as for animal health and welfare.
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