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Anencephaly is a congenital disease manifesting with the absence of the brain due to the failure of the cranial part of the neural tube to close during the embryonic stage. The disease may be accompanied by other anomalies and usually results in premature death. A stillborn puppy of a 2-year-old female Pomeranian dog is examined in this case. The lack of brain tissue and accompanying abnormal skull formation was noted macroscopically. The eyes were protruding out of their normal position (exophthalmos), and a bifid tongue together with a secondary cleft palate was present. On serial sections stained with Haematoxylin-Eosin, only the medulla spinalis among the central nervous system structures could be inspected microscopically. Immunohistochemistry staining revealed GFAP immunoreactivity in the astrocytic glial cells. NeuN immunoreactivity was detected in the neurons in the medulla spinalis and spinal ganglions. Incomplete retinal layers were observed on the eye sections stained with Haematoxylin-Eosin and NeuN. The case was concluded to be coherent with skull and nervous system congenital malformations rarely observed in dogs. To the best of our knowledge, this represents the first description of a dog with anencephaly, bifid tongue and cleft palate.

Pigs are the main host species for the pseudorabies virus, while it causes fatal encephalitis in many species including human being. The objective of this article is to report the rst clinical case of pseudorabies as well as isolation and molecular characterization of the virus from a hunting dog in Bursa province, Turkey. The dog showing clinical signs including pruritus and neurological signs as stumbling and inability to stand up compatible with pseudorabies. The virus isolate was obtained from supernatant of fresh tissue samples from cerebellum, cornu ammonis, spleen, salivary gland, and conjunctival swab, as well as serum, and buffy coat samples. The glycoprotein C region is targeted for viral DNA ampli cation. Pseudorabies virus genome was detected both in fresh tissues and supernatants of 3 rd passage on Vero cells. Number of PCR positive samples were dramatically increased after having cell culture inoculations. Genome sequencing revealed that the strain (Bursa-10303) isolated in Turkey where a non-endemic area is for the virus is dropped into clade A. This study con rms the presence of psedorabies infection in the wild life reservoirs in Turkey. Future studies may clarify the importance of the infection for Turkey where there is no common pig production.

The cownose ray, Rhinoptera bonasus, is currently listed as vulnerable and threatened species by the IUCN Red List due to overfishing and bycatch in worldwide resulted declines of the steep population declines of 30-49% in only 43 years. We aimed to present suitable dissection methods for a necropsy of cownose ray with a large, rhomboid-shaped wing-like pectoral disc and compressed dorsoventrally using a specific approach to observe external and internal anatomy. The fish were obtained from a public aquarium after natural death and were dissected with four incision area to open chondrocranium, gills, pericardial cavity and pleuroperitoneal cavity. The dorsal surface of the body is completely naked and smooth, greenish-brown colored, in contrast the ventral surface of the body is white. The liver fills most of the pleuroperitoneal cavity and covers the esophagus, stomach, duodenum, valvular intestine, spleen, kidney and uterus. The valvular intestine or spiral valve is one of the largest organs of the digestive system. In most rays, only the left ovary is developed and functional; therefore, the ovaries of the specimens are located on the left to the median line, close to the cranial end of the pleuroperitoneal cavity, and caudal to the stomach. In conclusion, the presented dissection techniques will enable to examine of the external and internal anatomy of cownose rays and will contribute to a suitable approach to organs.

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