Seroprevalance of Respiratory Viruses and Pestivirus Screening in Ruminant Species in Diyarbakır Region, Turkey

Kadiroğlu, Berfin; Alpay, Gizem; Yeşilbağ, Kadir

doi:10.30782/jrvm.559809

Cited by 2 publications

(2 citation statements)

References 34 publications

(28 reference statements)

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“…The individual seropositivity (59.78%) obtained in this study was in accordance with previous studies that reported seropositivity of BRSV in cattle ranged between 46.06% and 73% in Turkey (Yavru et al 2005 ; Yeşilbağ and Güngör, 2008 ; Kadiroğlu et al 2020 ). The highest seropositivity (73%) was found in Marmara region of Turkey.…”

Section: Discussionsupporting

confidence: 92%

“…There is limited knowledge available about the prevalence of BRSV infection (Yavru et al 2005 ; Yeşilbağ and Güngö, 2008 ; Kadiroğlu et al 2020 ) and genetic features of BRSV strains circulating in Turkey (Hacıoğlu et al 2019 ; Timurkan et al 2019 ; Yazici et al 2020 ). Furthermore, the possible risk factors for BRSV infection in cattle have not been studied in Turkey.…”

Section: Introductionmentioning

confidence: 99%

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Risk factors and genetic characterization of bovine respiratory syncytial virus in the inner Aegean Region, Turkey

İnce

Şevik

Ozgur

et al. 2021

Trop Anim Health Prod

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Bovine respiratory syncytial virus (BRSV) is one of the causative viral agents of the bovine respiratory disease complex. This study was conducted to determine the seropositivity and risk factors associated with BRSV infection and to evaluate the phylogenetic relatedness of the BRSVs in the inner Aegean region of Turkey. In this cross-sectional study, serum samples ( n = 557) and nasal swabs ( n = 21) were collected from cattle herds ( n = 43) between February 2018 and March 2019. A commercial indirect-ELISA kit was used for the detection of antibodies in the sera samples. Reverse-transcriptase PCR was used to detect viral RNA in nasal swabs. Nasal samples were also examined for the detection of bovine parainfluenza-3, bovine viral diarrhoea virus, and bovine herpesvirus 1 by molecular detection methods. Genetic characterization of the local BRSV field isolates was conducted by sequencing attachment glycoprotein (G) gene segment. Epidemiological data on potential risk factors were collected from each sampled herd during blood collection. All herds had at least one seropositive animal. After adjustment for assay sensitivity and specificity, the overall true seropositivity was 58.48% (95% CI: 53.32–63.47). BRSV RNA was detected in 2 of the 21 nasal swabs, whereas other infectious agents were not detected in the investigated samples. Phylogenetic analysis showed that the field isolates of BRSV obtained in this study belonged to subgroup III, but they were located on separate branch from previously characterised Turkish subgroup III isolates. BRSV field strains from this study displayed 3 new amino acid substitutions (P89S, D115G, and S165L) in the G protein chains compared to other main reference BRSV isolates, demonstrating that BRSV is still evolving. Generalised estimating equation model showed that there were positive associations between BRSV infection, age (OR = 2.36, p = 0.001), herd size (OR = 10.32, p < 0.001), herd type (OR = 8.97, p < 0.001), a past history of respiratory disease (OR = 4.06, p < 0.001). The results of this study revealed that BRSV infection is common among cattle herds in the inner Aegean region of Turkey. The obtained epidemiological and genetic data on BRSV infection from this study could be beneficial for designing effective biosecurity practices and vaccination strategies.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Risk factors and genetic characterization of bovine respiratory syncytial virus in the inner Aegean Region, Turkey

İnce

Şevik

Ozgur

et al. 2021

Trop Anim Health Prod

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Molecular Investigation of Bovine Viral Diarrhea Virus, Bovine Herpes Virus-1 and Bovine Herpes Virus-4 Infections in Abortion Cases of Cattle in Van District, Turkey

YILDIZ

Babaoğlu

2022

Van Veterinary Journal

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Abortions, fetal mummification, calf anomalies, and infertility problems are the majority of reproductive problems in cattle. Viruses play a significant role in the cause of these cases. In cattle, these agents are known as primary abortion agents and the most common of these agents are Bovine Viral Diarrhea Virus (BVDV), Bovine Herpes Virus Type 1 (BoHV-1), and Bovine Herpes Virus Type 4 (BoHV-4) infections. The objective of this research is to determine the possible role of BVDV, BoHV-1, and BoHV-4 as viral abortion agents in cattle housed in the Van district. For this, a total of 115 animal specimens (blood, serum, vaginal swab, vaginal fluid discharge, nasal swab, and abortion material) from 100 abortion, early embryonic deaths, and fertility problems cases in cattle over the age of 2-5 years old were collected. All samples for detection of BVDV, BoHV-1 and BoHV-4 genomes were tested by the Polymerase Chain Reaction (PCR) technique using specific primers encoding Panpesti 5'-UTR, Glycoprotein C (gC), and Glycoprotein B (gB) genes, respectively. Result out of the samples tested, %41.73 were positive for BVDV and all samples were negative for BoHV-1 and BoHV-4. In conclusion, the presence of BVDV in cattle in the Van region and its role in the occurrence of abortion cases was emphasized for the first time; consideration of viral abortions and determining the etiology of abortion cases and genital system problems needs to focus on the detection of persistent infected (PI) animals and the most effective way of vaccination of susceptible populations for prevention and control of infection.

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Seroprevalance of Respiratory Viruses and Pestivirus Screening in Ruminant Species in Diyarbakır Region, Turkey

Cited by 2 publications

References 34 publications

Risk factors and genetic characterization of bovine respiratory syncytial virus in the inner Aegean Region, Turkey

Risk factors and genetic characterization of bovine respiratory syncytial virus in the inner Aegean Region, Turkey

Molecular Investigation of Bovine Viral Diarrhea Virus, Bovine Herpes Virus-1 and Bovine Herpes Virus-4 Infections in Abortion Cases of Cattle in Van District, Turkey

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