Marine sponge Stylissa flabelliformis is a marine sponge species that is widely found in Indonesia. Marine sponges have been proven to have pharmacological activities including wound healing, cytotoxicity and antimicrobial activity. All these activities are related to its antioxidant potential. Antioxidants are important for human health due to its ability to protect cell damage caused by free radicals. The aim of this research was to study the antioxidant activity of marine sponge (Stylissa flabelliformis) and to observe the phytochemical compound of the marine sponge such as the phenolic and flavonoid content. Extraction was performed using cold maceration with 96% ethanol solvent and continued by rotary evaporator to get the concentrated extract. Antioxidants activity was analysed using DPPH (2,2-diphenyl-1-picrylhydrazyl) method, which examines the inhibitory concentration (IC50) of the ethanol extract. Total phenolic content (TPC) was examined using Gallic acid as the standard, while total flavonoid content (TFC) was examined using quercetin as the standard. The result showed a concentration-dependent antiradical activity by reduction of DPPH with an IC50 value 2000 µg/mL. Total phenolic content was 152,67 mg GAE/g, while the total flavonoid content was 155,79 mg QE/g.
The coastal areas of Lombok has very high diversity of macroalgae which are largely unexplored. Among them are the brown macroalgae Sargassum species which are well known for their biological potentials. In this study, we aim to evaluate 4 Sargassum species, S.cristaefolium, S. crassifolium, S. polycystum, and S. aquifolium for their antioxidant potential of Sargassum species found the west coastal Aare of Lombok, Indonesia. To determine the antioxidant capacity of macroalgal extracts, 2,2-diphenyl-1-picrylhydrazyl (DPPH) as well as total phenolic content (TPC) were measured. Extracts of the brown macroalgae are well known to have very active antioxidant activity. Among them S. cristaefolium and S.crassifolium provide stronger antioxidant activity compared to S.aquifolium and S.polycystum. Furthermore, correlations were found between TPC macroalgal and their DPPH scavenging activity. This indicates an important role of polyphenols as antioxidants. Overall, brown macroalgae from Lombok coastal area may be a good source of natural bioactive compounds.
Abstract.Collagen is a fibrous protein that has recently gained high attention from the pharmaceutical industry due to its benefits on the skin. Collagen can be isolated from various resources including marine sponges. Marine sponges are found in a large amount in Indonesia and has not been widely explored for its pharmacology benefits. Here we isolate collagen from a marine sponge Stylissa flabelliformis found in North Lombok Indonesia coast. The isolation of collagen was performed followed by total protein content analysis using modified Bradford protein assay and antioxidant activity measurement using 2,2-diphenyl-1-picrylhydrazyl(DPPH) assay. The total yield of the collagen isolate obtained was 3.5% and it had a total of 0.755mg/ml protein. DPPH assay has shown that the collagen isolate had antioxidant activity with an IC50 of 61.5±2.132 ppm. Based on Spearman correlation assay, the antioxidant activity was found to be correlated with the protein content of the isolate (r value=0.8). These results show the potency of using the collagen isolated from marine sponge Stylissa flabelliformis for further antioxidant benefits.
Seaweed is one of the marine-biota that is widely known for its uses, one of them is in the food functional. Functional food is a program that promotes a healthy diet so that it can prevent a disease when consuming food. With functional food, daily eating patterns can be maintained and also the content of the food consumed daily has a good effect on the body. It is known that in seaweed, especially brown algae (S. crassifolium), it contains antioxidants and other compounds that are good for the health. In this study, phytochemical content and proximate composition was evaluated in S. crassifolium. Including total flavonoid content and total phenolic content, antioxidant capacity both of DPPH and ABTS assay used three type of solvents such as Ethanol, n-Hexana, and ethyl acetate for comparable the best solvents. Also, proximate analysis to determine the content of moisture content, crude fat content, crude protein content, and carbohydrates content. The results showed that n-Hexana and ethyl acetate solvents were significantly than ethanol. Which mean both of them had highest values of total flavonoid content (TFC), total phenolic content (TPC), and antioxidant activity of DPPH radical scavenging activity and ABTS radical scavenging activity. The results of chemical composition in proximate compounds showed that S. crassifolium was suitable to be candidate of functional food.
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