Total Phenolic Content (TPC), Total Flavonoid Content (TFC) and Antioxidants Activity of Marine Sponge Stylissa flabelliformis Ethanol Extract

Abstract

Marine sponge Stylissa flabelliformis is a marine sponge species that is widely found in Indonesia. Marine sponges have been proven to have pharmacological activities including wound healing, cytotoxicity and antimicrobial activity. All these activities are related to its antioxidant potential. Antioxidants are important for human health due to its ability to protect cell damage caused by free radicals. The aim of this research was to study the antioxidant activity of marine sponge (Stylissa flabelliformis) and to observe the phytochemical compound of the marine sponge such as the phenolic and flavonoid content. Extraction was performed using cold maceration with 96% ethanol solvent and continued by rotary evaporator to get the concentrated extract. Antioxidants activity was analysed using DPPH (2,2-diphenyl-1-picrylhydrazyl) method, which examines the inhibitory concentration (IC50) of the ethanol extract. Total phenolic content (TPC) was examined using Gallic acid as the standard, while total flavonoid content (TFC) was examined using quercetin as the standard. The result showed a concentration-dependent antiradical activity by reduction of DPPH with an IC50 value 2000 µg/mL. Total phenolic content was 152,67 mg GAE/g, while the total flavonoid content was 155,79 mg QE/g.

Determination of Total Phenolic Content and Total Flavonoid Content of Nutmeg Flesh (Myristica Fragrans Houtt) Ethanol Extract from South Aceh Province

“…In this study using 96% ethanol solvent for the maceration process. The choice of ethanol solvent used is due to its polarity which can extract polar components in samples such as phenolic and flavonoid compounds [17]. 50.33 grams dried sample of nutmeg flesh powder (simplicia) was used and its extracted using 500 mL of 96% ethanol solvent, obtains 9.24 grams of ethanol extract or an extraction yield percentage of 18.35%, as shown in table 1.…”

“…Based on the results, ethanol solvent can dissolve polar compounds in the metabolites of nutmeg flesh [23]. Analysis of total phenolic and flavonoid levels using UV-visible spectrophotometer method was measured with wavelengths of 759 nm and 495 nm respectively [17]. Gallic acid standards were used to measure total phenolic levels and quarcetin standards to measure total flavonoid levels.…”

“…Data of total phenolic content that obtained is expressed as QE (quercetin equivalent), that is the equivalent number of standard milligrams of quercetin in gram samples. And data of total flavonoids content that obtained is expressed as GAE (galic acid equivalent), that is the equivalent number of standard milligrams of gallic acid in gram samples [17].…”