Klebsiella species known to exhibit important PGP traits like solubilization of phosphate, phytohormone production and good germination potential. In present study, based on the Phosphate solubilization and IAA production bacterial strain VRE36 was selected among the isolates collected from different sugarcane cultivar rhizosphere growing near Bardoli area for characterization and molecular identification through 16S rRNA gene sequence, which confirms the isolate as Klebsiella pneumoniae. The phosphate solubilization index of the isolate recorded was 3.9 and quantitative estimation reveals 17.4±1.78µg/ml release of phosphate in NBRIP broth. The high amount of IAA produced was 45.32±2.46µg/ml after 96 hour incubation at 37 °C. In seed germination assay with V radiate, C tetragonoloba and V unguiculata, treatments with bacteria were supported good plant height, dry weight and fresh weight when compared with controls. Maximum percentage of germination was recorded in V radiate (97.78%). The improved seedling parameters of the inoculated crop seeds indicated the potential of this isolate to be used in a bio-fertilizer formulation for sustainable production.
Fermentation technology plays a very important role in the field of Biotechnology. Production of various enzymes, antibiotics, single cell proteins, various food products, etc is carried out in a vessel called as the bioreactor which is the seed of fermentation technology. The project includes study of Pectinase production using submerged fermentation and various parameters like different substrate, different strains and fermentation time to obtain the maximum yield of Pectinase. To achieve this various research is being conducted. Aspergillus Niger species was being used in this work for the production of Pectinase.
Highlights
A total of 226 isolates were screened against three strains (cfNAV, cfCHA and cf8436) of
C. falcatum
by dual culture technique. Selected Twenty-Six bacteria characterized of morphology, biochemical activity, PGP activity, antifungal potential and 16S rRNA gene sequence. These isolates belonged to proteobacteria (13), firmicutes (10) and bacterioidetes (03) respectively.
Ochrobactrum intermedium
(TRD14),
Acinetobacter
sp (PK9),
Bacillus
sp (RSC29 and KR91) and
Escherichia
sp (VRE34) selected for green house study. The most promising results in suppression of the disease as well as plant growth were observed in treatment withVRE34. The plant height and stem diameter were increased from 13.27 ± 0.67 inches to 24.03 ± 1.40 inches and from 6.07 ± 0.45 mm to 9.87 ± 0.93 mm.
Isolates identified in this study could be used as an alternative to chemical fungicides to control red rot pathogen of sugarcane plants. However, detailed investigations on their inoculations in the field to confirm its growth promotion potency and biocontrol efficacy under natural environmental and soil conditions shall make these strains as important bioinoculants for integrated disease management of red rot disease in sugarcane.
Laccases are multi copper oxidases having wide substrate specificity mainly found in white-rot fungi, which are the only microorganisms able to degrade the whole wood components. In contrast to most enzymes, which are generally very substrate specific, laccases act on a surprisingly broad range of substrates, including diphenols, polyphenols, different substituted phenols, diamines, aromatic amines, benzenethiols and even some inorganic compounds such as iodine. As they are capable of degrading a wide variety of compounds they are commercially very significant. This project aims at studying the production optimization of laccase using different carbon sources.
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