Molecular identification and biocontrol activity of sugarcane rhizosphere bacteria against red rot pathogen Colletotrichum falcatum

Abstract

Highlights A total of 226 isolates were screened against three strains (cfNAV, cfCHA and cf8436) of C. falcatum by dual culture technique. Selected Twenty-Six bacteria characterized of morphology, biochemical activity, PGP activity, antifungal potential and 16S rRNA gene sequence. These isolates belonged to proteobacteria (13), firmicutes (10) and bacterioidetes (03) respectively. Ochrobactrum intermedium (TRD14), Acinetobacter sp (PK9), Bacillus sp (RSC29 and KR91) and Escherichia sp (VRE34) selected for green house study. The most promising results in suppression of the disease as well as plant growth were observed in treatment withVRE34. The plant height and stem diameter were increased from 13.27 ± 0.67 inches to 24.03 ± 1.40 inches and from 6.07 ± 0.45 mm to 9.87 ± 0.93 mm. Isolates identified in this study could be used as an alternative to chemical fungicides to control red rot pathogen of sugarcane plants. However, detailed investigations on their inoculations in the field to confirm its growth promotion potency and biocontrol efficacy under natural environmental and soil conditions shall make these strains as important bioinoculants for integrated disease management of red rot disease in sugarcane.

Diffusible and volatile organic compounds produced by avocado rhizobacteria exhibit antifungal effects against Fusarium kuroshium

“…and Pseudomonas spp. in particular are frequently cited among the most successful biocontrol agents [19][20][21][22]. Recent findings from our research group have confirmed the in vitro potential of avocado rhizobacteria to act as biocontrol agents of fungi responsible of FD [23,24], although the mechanisms behind the antagonism still need to be deciphered.…”

Inoculation With Growth-Promoting Bacteria Associated With the Reduction of Phosphate Fertilization in Sugarcane

“…The antagonistic strains of Bacillus sp. stand relatively efficient than other biocontrol agents because of their excessive sporulation, prolonged life, improved plant nutrition and potential in controlling plant diseases (Hassan et al, 2010;Patel et al, 2019). Zarei et al (2019) studied P. fluorescens strains and different irrigation levels in sweet corn.…”

Current and Prospective Strategies on Detecting and Managing Colletotrichumfalcatum Causing Red Rot of Sugarcane

“…In recent times, different genera of bacterial such as Enterobacter, Pseudomonas, Burkholderia, Bacillus, Gluconaceto bacter and Ochrobactrum are known (in-vivo and in-vitro trials) to effectively inhibit C. falcatum in the sugarcane rhizosphere [86][87][88]. Patel et al [16] conducted an in-vivo study against three strains of C. falcatum. The findings showed that Ochrobactrum intermedium (TRD 14) effectively regulated the pathogenicity of C. falcatum (cfNAV) and it also enhanced the growth of sugarcane plants by 8.2%.…”

“…However, because of many overlapping characteristics within the species' complex, identification using morphologic techniques is not enough. Moreover, managing red rot disease in the field is difficult as the genetic makeup of this fungus keeps changing [16]. Therefore, accurate and rapid identification of Colletotrichum is essential.…”