Klebsiella pneumoniae VRE36 as a PGPR isolated from Saccharum officinarum cultivar Co99004

Abstract

Klebsiella species known to exhibit important PGP traits like solubilization of phosphate, phytohormone production and good germination potential. In present study, based on the Phosphate solubilization and IAA production bacterial strain VRE36 was selected among the isolates collected from different sugarcane cultivar rhizosphere growing near Bardoli area for characterization and molecular identification through 16S rRNA gene sequence, which confirms the isolate as Klebsiella pneumoniae. The phosphate solubilization index of the isolate recorded was 3.9 and quantitative estimation reveals 17.4±1.78µg/ml release of phosphate in NBRIP broth. The high amount of IAA produced was 45.32±2.46µg/ml after 96 hour incubation at 37 °C. In seed germination assay with V radiate, C tetragonoloba and V unguiculata, treatments with bacteria were supported good plant height, dry weight and fresh weight when compared with controls. Maximum percentage of germination was recorded in V radiate (97.78%). The improved seedling parameters of the inoculated crop seeds indicated the potential of this isolate to be used in a bio-fertilizer formulation for sustainable production.

The Phsyiological Character of Bacteria Isolated From Banana’s Rhizosphere From Malaka, East Nusa Tenggara, and Their Role on Plant Growth Promotion on Marginal Land

“…Klebsiella pneumoniae, a human pathogen, also showed a plant growth promoting characteristic, in a high level producing IAA and solubilizing phosphate. Similar result reported by Bhardwaj, Shah, Joshi, and Patel (2017) that Klebsiella pneumoniae VRE36 associated with sugarcane exhibit important plant growth promoting traits. In soil, most phosphate occur as insoluble form that cannot be absorbed by plants (Rengel and Marschner, 2005).…”

Isolation and Characterization of Plant Growth Promoting Rhizobacteria Associated with Saccharum officinarum L.

“…Among all eight isolates F372 (15 mg L -1 ) was found best isolates as phosphate solubilizers followed by ESB4 (14 mg L -1 ) ( Table 5). Similar consistent results of phosphate solubilization in both agar and broth assay were observed earlier [1,23]. Quantification of IAA was carried out for eight different isolates.…”

“…Importance of sugar in human diet needs no introduction; it has become a part and parcel of daily life. As sugarcane is a long duration crop and faces many biotic and abiotic stresses during growth phase, the microorganism associated with sugarcane roots may be potent and useful for application to promote plant growth [1]. Soil microorganisms play a significant role in regulating the dynamics of organic matter, decomposition and the availability of plant nutrients such as Nitrogen (N), Phosphorous (P) and Sulfur (S).…”

Genomic insights of aromatic hydrocarbon degrading Klebsiella pneumoniae AWD5 with plant growth promoting attributes: a paradigm of soil isolate with elements of biodegradation

“…In addition, AWD5 has pqqBCDEF locus, which directed the synthesis of PQQ, a co-factor of glucose dehydrogenase holoenzyme (GDH) (Meulenberg et al 1992 ). Bhardwaj et al ( 2017 ) reported that K. pneumoniae VRE36 produced 45 µg/ml of IAA and estimated 17.4 µg/ml release of available phosphate in NBRIP medium.…”

“…It had been reported that K. pneumoniae strains were found to enhance the plant growth, seed germination rate in maize, wheat, sugarcane, etc. (Sachdev et al 2009 ; Kuan et al 2016 ; Bhardwaj et al 2017 ).…”