Effect of abiotic elicitation and pathway precursors feeding over terpenoid indole alkaloids production in multiple shoot and callus cultures of Catharanthus roseus

Sharma, Abhishek; Mathur, Anmol; Ganpathy, Jawahar; Joshi, B; Patel, Prittesh

doi:10.2478/s11756-019-00202-5

Cited by 23 publications

(8 citation statements)

References 45 publications

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“…Biotic elicitors such as chitosan and its oligomers obtained from fungal cell wall (Katiyar et al, 2015) and plant gums (Dass and Ramawat, 2009) have shown strong elicitation effect in cell cultures for the production of secondary metabolites. Previous studies have reported increased production of indole alkaloids in cell suspension cultures of Catharanthus roseus (Liang et al, 2018;Sharma et al, 2019) and taxol and related taxanes in suspension cultures of Taxus sp. (Vidal-Limon et al, 2016;Sarmadi et al, 2018).…”

Section: Introductionmentioning

confidence: 93%

Effect of biotic elicitors on polyphenol production in Cayratia trifolia cell suspension cultures analyzed by HPLC

Arora¹,

Joshi²,

Kanthaliya³

et al. 2020

bta

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Cayratia trifolia Linn. Domin Syn. Vitis trifolia (family: Vitaceae) contains polyphenols as major bioactive compounds. Plant polyphenolics, such as stilbenes, possess a broad spectrum of pharmacological and therapeutic effects, including antioxidative, anticancerous, antiatherosclerotic, cardioprotective, hepatoprotective, and neuroprotective effects. Elicitation is one of the important strategies to enhance secondary metabolite production in cell suspension cultures. In the present study, the effect of two biotic elicitors, namely one fungal (Fusarium sp., and Helminthosporium sp.) extract and another higher plant-originated gum (Anogeissus latifolia), was evaluated for enhanced production of stilbenes in cell suspension culture of C. trifolia. For the fungal elicitor, the cell suspension cultures of C. trifolia were treated with varying concentrations of Fusarium extract (FE) and Helminthosporium extract (HE) on the 7 th day of culture. Increasing concentration of FE in the medium resulted in slightly decreased cell growth but increased the production of stilbenes, with the optimal concentration at 1000 mg/l. This increase in the yield of stilbenes was-5 times (9.30 mg/l) higher than the control value. HE was less effective than FE in increasing stilbene production at optimal concentrations. The addition of 50 mg/l A. latifolia plant gum sieved through a 150 mesh (0.1 mm) to C. trifolia cell culture enhanced stilbene accumulation and yield by 8-to 9-fold over the control value.

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Section: Introductionmentioning

confidence: 93%

Effect of biotic elicitors on polyphenol production in Cayratia trifolia cell suspension cultures analyzed by HPLC

Arora¹,

Joshi²,

Kanthaliya³

et al. 2020

bta

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“…Adding exogenous substances, such as precursors, elicitors, nutrient elements, and signal molecules, to plant tissue culture systems is an effective method to improve the plant TIA content (Jeet et al, 2020). For example, the accumulation of vinblastine was improved by treatment with tryptophan (Trp) in multiple shoot and callus cultures of Catharanthus roseus (Sharma et al, 2019), and MeJA and JA can be used as signal molecules to regulate the biosynthesis of plant secondary metabolites, such as nicotine, anthocyanin, artemisinin, and TIAs (Wasternack and Strnad, 2019).…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic analysis of genes related to alkaloid biosynthesis and the regulation mechanism under precursor and methyl jasmonate treatment in Dendrobium officinale

Chen

Wei²,

Fan³

et al. 2022

Front. Plant Sci.

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Dendrobium officinale is both a traditional herbal medicine and a plant of high ornamental and medicinal value. Alkaloids, especially terpenoid indole alkaloids (TIAs), with pharmacological activities are present in the tissues of D. officinale. A number of genes involved in alkaloid biosynthetic pathways have been identified. However, the regulatory mechanisms underlying the precursor and methyl jasmonate (MeJA)-induced accumulation of alkaloids in D. officinale are poorly understood. In this study, we collected D. officinale protocorm-like bodies (PLBs) and treated them with TIA precursors (tryptophan and secologanin) and MeJA for 0 (T0), 4 (T4) and 24 h (T24); we also established control samples (C4 and C24). Then, we measured the total alkaloid content of the PLBs and performed transcriptome sequencing using the Illumina HiSeq 2,500 system. The total alkaloid content increased significantly after 4 h of treatment. Go and KEGG analysis suggested that genes from the TIA, isoquinoline alkaloid, tropane alkaloid and jasmonate (JA) biosynthetic pathways were significantly enriched. Weighted gene coexpression network analysis (WGCNA) uncovered brown module related to alkaloid content. Six and seven genes related to alkaloid and JA bisosynthetic pathways, respectively, might encode the key enzymes involved in alkaloid biosynthesis of D. officinale. Moreover, 13 transcription factors (TFs), which mostly belong to AP2/ERF, WRKY, and MYB gene families, were predicted to regulate alkaloid biosynthesis. Our data provide insight for studying the regulatory mechanism underlying TIA precursor and MeJA-induced accumulation of three types of alkaloids in D. officinale.

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“…The production of C. roseus phytochemicals has been accomplished using callus, cell suspension, somatic embryo, and transformed or non-transformed root and shoot cultures by optimizing the chemical and physical parameters [7,9,12,13]. Several alkaloids, such as ajmalicine, vindoline, catharanthine, vinblastine, and vincristine, were successfully obtained from C. roseus shoot cultures [14][15][16][17][18][19][20]. Phenolics are essential secondary metabolites obtained from various plant parts and have a wide range of biological activities [21].…”

Section: Introductionmentioning

confidence: 99%

“…Explants with vegetative meristems are often suitable for axillary shoot multiplication and clonal propagation. Direct multiple shoot regeneration has been achieved using nodal segments, shoot tips, and axillary buds from C. roseus seedlings and mature plants [11,20,[24][25][26][27][28]. Cytokinins play an essential role in shoot development.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Composition, Antioxidant Capacity, and Enzyme Inhibitory Activity in Callus, Somaclonal Variant, and Normal Green Shoot Tissues of Catharanthus roseus (L) G. Don

Lee

Zengin

et al. 2020

Molecules

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This study aimed to investigate the impact of plant growth regulators, sucrose concentration, and the number of subcultures on axillary shoot multiplication, in vitro flowering, and somaclonal variation and to assess the phytochemical composition, antioxidant capacity, and enzyme inhibitory potential of in vitro-established callus, somaclonal variant, and normal green shoots of Catharanthus roseus. The highest shoot induction rate (95.8%) and highest number of shoots (23.6), with a mean length of 4.5 cm, were attained when the C. roseus nodal explants (0.6–1 cm in length) were cultivated in Murashige and Skoog (MS) medium with 2 µM thidiazuron, 1 µM 2-(1-naphthyl) acetic acid (NAA), and 4% sucrose. The in vitro flowering of C. roseus was affected by sucrose, and the number of subcultures had a significant effect on shoot multiplication and somaclonal variation. The highest levels of phenolics and flavonoids were found in normal green shoots, followed by those in somaclonal variant shoots and callus. The phytochemicals in C. roseus extracts were qualified using liquid chromatography–tandem mass spectrometry. A total of 39, 55, and 59 compounds were identified in the callus, somaclonal variant shoot, and normal green shoot tissues, respectively. The normal green shoot extracts exhibited the best free radical scavenging ability and reducing power activity. The strongest acetylcholinesterase inhibitory effects were found in the callus, with an IC50 of 0.65 mg/mL.

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Effect of abiotic elicitation and pathway precursors feeding over terpenoid indole alkaloids production in multiple shoot and callus cultures of Catharanthus roseus

Cited by 23 publications

References 45 publications

Effect of biotic elicitors on polyphenol production in Cayratia trifolia cell suspension cultures analyzed by HPLC

Effect of biotic elicitors on polyphenol production in Cayratia trifolia cell suspension cultures analyzed by HPLC

Transcriptomic analysis of genes related to alkaloid biosynthesis and the regulation mechanism under precursor and methyl jasmonate treatment in Dendrobium officinale

Phytochemical Composition, Antioxidant Capacity, and Enzyme Inhibitory Activity in Callus, Somaclonal Variant, and Normal Green Shoot Tissues of Catharanthus roseus (L) G. Don

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