Effect of biotic elicitors on polyphenol production in Cayratia trifolia cell suspension cultures analyzed by HPLC

Abstract

Cayratia trifolia Linn. Domin Syn. Vitis trifolia (family: Vitaceae) contains polyphenols as major bioactive compounds. Plant polyphenolics, such as stilbenes, possess a broad spectrum of pharmacological and therapeutic effects, including antioxidative, anticancerous, antiatherosclerotic, cardioprotective, hepatoprotective, and neuroprotective effects. Elicitation is one of the important strategies to enhance secondary metabolite production in cell suspension cultures. In the present study, the effect of two biotic elicitors, namely one fungal (Fusarium sp., and Helminthosporium sp.) extract and another higher plant-originated gum (Anogeissus latifolia), was evaluated for enhanced production of stilbenes in cell suspension culture of C. trifolia. For the fungal elicitor, the cell suspension cultures of C. trifolia were treated with varying concentrations of Fusarium extract (FE) and Helminthosporium extract (HE) on the 7 th day of culture. Increasing concentration of FE in the medium resulted in slightly decreased cell growth but increased the production of stilbenes, with the optimal concentration at 1000 mg/l. This increase in the yield of stilbenes was-5 times (9.30 mg/l) higher than the control value. HE was less effective than FE in increasing stilbene production at optimal concentrations. The addition of 50 mg/l A. latifolia plant gum sieved through a 150 mesh (0.1 mm) to C. trifolia cell culture enhanced stilbene accumulation and yield by 8-to 9-fold over the control value.

Evaluation of Total Isoflavones in Chickpea (Cicer arietinum L.) Sprouts Germinated under Precursors (p-Coumaric Acid and L-Phenylalanine) Supplementation

“…HPLC is one of the most reliable methods to determine the changes in phenolic content in the presence of elicitor compounds or under different growth conditions [23,68]. The HPLC chromatogram (Figure 5A-D) showed an increase in the concentration of some phenolic compounds in the presence of both p-CA and Phe supplementations.…”

“…In recent years, researchers have explored strategies to further enhance the bioactive content of legumes by manipulating the germination conditions. One such approach involves the use of exogenous precursors or elicitor compounds to stimulate the synthesis of specific bioactive compounds [23][24][25][26][27]. Precursors are compounds that can be metabolized within a plant system to produce targeted bioactive compounds [28].…”

Effect of Biotic Elicitors on the Growth, Antioxidant Activity and Metabolites Accumulation in In Vitro Propagated Shoots of Pueraria tuberosa

“…There is sufficient evidence that the culture medium composition greatly influences the micropropagation of Pueraria species and determines the growth, development, and metabolite composition of plant tissues [ 29 ]. The use of biotic elicitors has been shown to facilitate cell growth and increase the accumulation of bioactive molecules within cells and tissues [ 30 , 31 , 32 ]. Elicitor concentration is a crucial aspect of productive elicitation, and plant species differ in their optimal concentration [ 33 ].…”

Effect of sucrose as an elicitor in increasing quercetin-3-O-rhamnoside (quercitrin) content of chrysanthemum (Chrysanthemum morifolium Ramat) callus culture based on harvest time differences

“…The biosynthesis of plant secondary metabolites is influenced by environmental stress, but the accumulation of desired metabolites can be stimulated by the application of specific precursors and elicitors (Wang et al, 2015). The synthesis and accumulation of secondary metabolites in cell and organ cultures can be triggered by the application of elicitors into culture media (Naik and Al-Khayri, 2016;Rora et al, 2020).…”