This work describes an electrochemical genosensor for detection of genomic RNA of Zika virus in real samples of infected patients, using a new platform based on graphite electrodes modified with electrochemically reduced graphene oxide and polytyramine‐conducting polymer. The developed genosensor was suitable for differentiation between samples of healthy and infected patients with Zika virus by differential pulse voltammetry, detecting up to 0.1 fg/mL (1.72 copies/mL), showing good stability (about 60 days), rapid analysis (about 20 min) and potential for filling the lack of practical diagnostic methods for Zika virus.
This work describes the preparation of a reusable electrochemical immunosensor for detection of C-reactive protein (CRP), based in modified carbon electrode with reduced graphene oxide and polytyramine as strategy for anchoring the anti-CRP probe. This immunosensor was able to distinguish different concentrations of CRP target in human serum, by differential pulse voltammetry, showing a linear response from 1.09 to 100 μg L À 1. The performance of the immunosensor allowed its application in four consecutive assays, without activity loss. Analyses by atomic force microscopy, scanning electron microscopy and electrochemical impedance spectroscopy corroborate with voltammetric analysis. The immunosensor showed selectivity, stability and reusability, being a promising platform for point-of-care quantification of CRP.
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