Reusable Immunosensor for Detection of C‐reactive Protein in Human Serum

Ribeiro, Sandro Henrique Dias; Alves, Lívia Maria; Flauzino, José M.R.; Moço, Anna C.R.; Segatto, Mônica Silva; Silva, Jonathan P.; Borges, Lizandra F. A.; Madurro, João M.; Madurro, Ana G. B.

doi:10.1002/elan.202000043

Cited by 12 publications

(11 citation statements)

References 38 publications

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“…Carbon-based materials are among the most widely used materials for electrode modification. Ribeiro et al developed a reusable immunosensor by immobilizing antibodies on carbon-modified electrodes through non-covalent interactions and utilized them to detect CRP [30]. As shown in Figure 1A, the graphite electrode was first modified with graphene by electrochemical reduction using GO.…”

Section: Physical Adsorption Of Antibodiesmentioning

confidence: 99%

“…The fabricated immunosensor showed an LOD of 886 fg/mL and 86 fg/mL for DPV and SWV, respectively, whereas the linear range was 1-300 pg/mL. Reproduced with permission from [30]. Copyright (2020) John Wiley and Sons., Inc. (B) Immunosensor with non-covalently immobilized antibodies on AuNPs.…”

Section: Physical Adsorption Of Antibodiesmentioning

confidence: 99%

“…(A) Schematic diagram of CRP immunosensor. Antibodies were immobilized on the polymer-modified electrode by the charge interaction.Reproduced with permission from[30]. Copyright (2020) John Wiley and Sons., Inc. (B) Immunosensor with non-covalently immobilized antibodies on AuNPs.…”

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confidence: 99%

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Recent Progress in Electrochemical Immunosensors

Kim

Park

2021

Biosensors

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Biosensors used for medical diagnosis work by analyzing physiological fluids. Antibodies have been frequently used as molecular recognition molecules for the specific binding of target analytes from complex biological solutions. Electrochemistry has been introduced for the measurement of quantitative signals from transducer-bound analytes for many reasons, including good sensitivity. Recently, numerous electrochemical immunosensors have been developed and various strategies have been proposed to detect biomarkers. In this paper, the recent progress in electrochemical immunosensors is reviewed. In particular, we focused on the immobilization methods using antibodies for voltammetric, amperometric, impedimetric, and electrochemiluminescent immunosensors.

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Section: Physical Adsorption Of Antibodiesmentioning

confidence: 99%

Section: Physical Adsorption Of Antibodiesmentioning

confidence: 99%

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Recent Progress in Electrochemical Immunosensors

Kim

Park

2021

Biosensors

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“…Thus, this LOD offers a great practical benefit, as it permits extensive sample dilution minimizing the intense matrix effects of clinical samples. [ 31–33 ] Figure 5B illustrates the amperometric responses for successive additions of H 2 O 2 in 3D e‐well and the respective calibration plot ( r 2 = 0.999) in the concentration range of 1.5–13.5 mmol L −1 . In the case of H 2 O 2 determination, the e‐wells were used as‐printed, without any modification of WE with nanoparticles or enzyme for enhanced sensitivity.…”

Section: Resultsmentioning

confidence: 99%

“…The bioanalytical capability of the 3D e‐wells to QD‐based bioassays is demonstrated through the determination C‐reactive protein (CRP) in artificial blood, as this protein is a critical diagnostic biomarker for inflammation, infection, and cardiovascular disease. [ 31–33 ] A sandwich‐type immunoassay is conducted in the 3D e‐well using biotinylated CRP reporter antibody labeled with streptavidin (STV)‐conjugated Cd‐based QDs. The quantification of the target protein is performed at the 3D e‐well after the acidic dissolution of the QDs and ASV determination of the Cd(II) released (Figure 1C).…”

Section: Introductionmentioning

confidence: 99%

3D Printed Bioelectronic Microwells

Katseli

Angelopoulou

Kokkinos

2021

Adv Funct Materials

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The in‐house and on‐demand fabrication of electrochemical integrated biosensors is a great challenge, especially in the field of modern point‐of‐care diagnostics. 3D printing technology allows the production of specialized electronic devices adapted to the required conditions, and 3D printed thermoplastic electrodes have shown hopeful achievements mainly in enzymatic bioassays. This work describes a novel configuration of integrated all‐3D‐printed electrochemical microtitration wells (e‐wells) for direct quantum dot‐based (QDs) and enzymatic bioassays. The e‐wells enable the in situ development of complete bioassays, that is, from sample addition to biomarker detection, without the need for external equipment other than a micropipette and a detector. The bioanalytical capability of the 3D e‐wells is demonstrated through the voltammetric bioassay of C‐reactive protein employing biotinylated reporter antibody and streptavidin‐conjugated CdSe/ZnS QDs. In addition, in order to extend their scope to enzymatic biosensing, e‐wells are applied to the amperometric determination of hydrogen peroxide by‐products, demonstrating their universal applicability in electrochemical bioassays.

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Electro‐immunosensor for ultra‐sensitive determination of cardiac troponin I based on reduced graphene oxide and polytyramine

Brussasco

Guedes

Moço

et al. 2022

J of Molecular Recognition

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This work reports the construction of a novel nanostructured immunosensor for detection of the troponin I biomarker (cTnI). Anti-troponin I antibody was anchored on the modified graphite electrode with reduced graphene oxide and polytyramine for detection of troponin I in serum samples. The performance of the electro-immunosensor was evaluated by differential pulse voltammetry. The immunosensor presented a wide work range, from 4 ng mL À1 to 4 pg mL À1 , whose detection limit (4 pg mL À1 ) is significantly lower than the basal level in human serum, and maintained 100% of response after 30 days of storage. Moreover, the immunosensor showed good selectivity for detection of cTnI in real sample containing interfering substances and specificity of response to cTnI in the serum of healthy and sick patients, and demonstrated the possibility of reuse for two consecutive analyses, in addition to using a simplified and inexpensive platform when compared to other devices, demonstrating them excellent potential for application in diagnosis in the early stages of acute myocardial infarction.

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Reusable Immunosensor for Detection of C‐reactive Protein in Human Serum

Cited by 12 publications

References 38 publications

Recent Progress in Electrochemical Immunosensors

Recent Progress in Electrochemical Immunosensors

3D Printed Bioelectronic Microwells

Electro‐immunosensor for ultra‐sensitive determination of cardiac troponin I based on reduced graphene oxide and polytyramine

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