Carbon ink-based electrodes modified with nanocomposite as a platform for electrochemical detection of HIV RNA

Moço, Anna C.R.; Neto, João A.S.; Moraes, Dayane Dotto de; Guedes, Pedro H.G.; Brussasco, Jéssica G.; Flauzino, José M.R.; Luz, Luiz F.G.; Soares, Márcia Maria Costa Nunes; Madurro, João M.; Brito‐Madurro, Ana G.

doi:10.1016/j.microc.2021.106739

Cited by 8 publications

(4 citation statements)

References 41 publications

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“…Hence, they are a perfect choice as transducers for biosensors. In this sense, we can find several works using spherical or other shape gold nanoparticle for DNA biosensors [ 7 , [18] , [19] , [20] ] but as far as we know there have not been reported the use of mixtures of gold nanomaterials (AuNMs) of different shapes (gold nanoparticles (AuNPs) and gold nanotriangles (AuNTs)) as platforms for DNA biosensor development.…”

Section: Introductionmentioning

confidence: 99%

Electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection

Gutiérrez-Gálvez

Caño

Menéndez-Luque

et al. 2022

Talanta

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This work focuses on the development of an electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection. Gold nanomaterials (AuNMs), specifically, a mixture of gold nanotriangles (AuNTs) and gold nanoparticles (AuNPs), are used to modified disposable electrodes that serve as an improved nanostructured electrochemiluminescent platform for DNA detection. Carbon nanodots (CDs), prepared by green chemistry, are used as coreactants agents in the [Ru(bpy) 3 ] 2+ anodic electrochemiluminescence (ECL) and the hybridization is detected by changes in the ECL signal of [Ru(bpy) 3 ] 2+ /CDs in combination with AuNMs nanostructures. The biosensor is shown to detect a DNA sequence corresponding to SARS-CoV-2 with a detection limit of 514 aM.

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Section: Introductionmentioning

confidence: 99%

Electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection

Gutiérrez-Gálvez

Caño

Menéndez-Luque

et al. 2022

Talanta

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“…While nucleic acid tests can detect HIV earlier (10-33 days after exposure), these laboratory-based tests require intravenous blood draw, expensive equipment, time-consuming protocols, cold-chain reagent storage and trained personnel. As such, researchers are working to further advance rapid HIV testing to detect the virus at the acute phase [10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Stakeholder-engaged development of a rapid test for detection of acute HIV infection

Rodriguez,

Balian,

Kataki

et al. 2024

Preprint

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Background/Objective: The utilization of rapid HIV tests has been effective at reducing transmission rates in high-risk populations by allowing individuals to receive diagnosis in as little as one minute and begin treatment. However, no current rapid tests can detect HIV immediately after infection in the acute HIV infection (AHI) phase, when the virus is at its most infectious, and instead require a waiting period of up to 90 days after exposure. Rapid HIV tests to detect AHI are currently under development. Investigation of stakeholder perspectives and context-specific needs are critical to ensure successful translation of novel AHI tests. The objectives of this study were to 1) understand context-specific factors such as barriers to HIV testing in Indiana, a state with one of 48 prioritized counties for HIV elimination; 2) assess the acceptability of a novel rapid AHI test, and 3) identify key implementation considerations for such a device, including ideal end-users. Methods: Semi-structured in-depth interviews were conducted with staff (n = 14) and clients (n = 5) of Indiana-based organizations that conduct HIV testing, including syringe service programs. Utilizing human-centered design frameworks, interview guides were developed and tailored to each participant group to understand their experiences with HIV testing, perspectives on a novel rapid AHI test in development, and preferences for self-testing versus testing by a community health worker (CHW) or a peer recovery coach. Thematic analysis was conducted to identify major themes, including barriers to HIV testing and perceived benefits and concerns of the proposed AHI test. Results: Overall acceptability for a novel AHI rapid test was high with a greater preference for CHW/Peer-led testing. While self-testing was not a preferred modality, it was still seen as a potential tool to reach and address key barriers among high-risk individuals. Key considerations for implementation emphasized accuracy, cost-effectiveness, ease of use, ensuring access to counseling, education, and navigation to care while maintaining a human element to self-testing. Conclusion: Stakeholder engagement is meaningfully informing the design, development, and implementation of rapid AHI testing in order to facilitate adoption among populations at high-risk for HIV.

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“… 10,11 Because of the limitations of label-free electrochemical detection in terms of sensitivity, 12 we have employed a redox DNA hybridization indicator to monitor the current signals from DNA hybridization events. Various redox DNA indicators that are available include ethidium bromide 13 (EtBr), Hoechst 33258, 14 methylene blue, acridine orange, 15 RuHex 3+ , 16 ferrocene, 17 daunomycin, 18 Meldola blue, 19 and Co(phen) 3 3+ , 20 and others have also been documented. Erdem and colleagues 21 reported the first work on the high discrimination potential of MB binding properties, and they found out that MB demonstrated higher affinity toward guanine bases in ssDNA (before DNA hybridization) compared to dsDNA (after DNA hybridization).…”

Section: Introductionmentioning

confidence: 99%