Electrochemical Detection of Zika Virus in Biological Samples: A Step for Diagnosis Point‐of‐care

Moço, Anna C.R.; Guedes, Pedro H.G.; Flauzino, José M.R.; Silva, Heliane S. da; Vieira, Jussara G.; Castro, Ana Cristina Honorato de; Gomes, Érica V. R.; Tolentino, Fernanda Modesto; Soares, Márcia Maria Costa Nunes; Madurro, João M.; Brito‐Madurro, Ana G.

doi:10.1002/elan.201900068

Cited by 32 publications

(22 citation statements)

References 38 publications

(31 reference statements)

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“…The LOD reached 0.1 fg mL −1 (1.72 copies mL −1 ). The advantages of this method were rapid analysis (20 min), a reliable response even after 60 days of storage and the possibility of miniaturization ( Moço et al, 2019 ).…”

Section: Graphene-based Materials In Virus Sensingmentioning

confidence: 99%

Human virus detection with graphene-based materials

Vermisoglou

Panáček

Jayaramulu

et al. 2020

Biosensors and Bioelectronics

153

117

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Our recent experience of the COVID-19 pandemic has highlighted the importance of easy-to-use, quick, cheap, sensitive and selective detection of virus pathogens for the efficient monitoring and treatment of virus diseases. Early detection of viruses provides essential information about possible efficient and targeted treatments, prolongs the therapeutic window and hence reduces morbidity. Graphene is a lightweight, chemically stable and conductive material that can be successfully utilized for the detection of various virus strains. The sensitivity and selectivity of graphene can be enhanced by its functionalization or combination with other materials. Introducing suitable functional groups and/or counterparts in the hybrid structure enables tuning of the optical and electrical properties, which is particularly attractive for rapid and easy-to-use virus detection. In this review, we cover all the different types of graphene-based sensors available for virus detection, including, e.g., photoluminescence and colorimetric sensors, and surface plasmon resonance biosensors. Various strategies of electrochemical detection of viruses based on, e.g., DNA hybridization or antigen-antibody interactions, are also discussed. We summarize the current state-of-the-art applications of graphene-based systems for sensing a variety of viruses, e.g., SARS-CoV-2, influenza, dengue fever, hepatitis C virus, HIV, rotavirus and Zika virus. General principles, mechanisms of action, advantages and drawbacks are presented to provide useful information for the further development and construction of advanced virus biosensors. We highlight that the unique and tunable physicochemical properties of graphene-based nanomaterials make them ideal candidates for engineering and miniaturization of biosensors.

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Section: Graphene-based Materials In Virus Sensingmentioning

confidence: 99%

Human virus detection with graphene-based materials

Vermisoglou

Panáček

Jayaramulu

et al. 2020

Biosensors and Bioelectronics

153

117

View full text Add to dashboard Cite

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“…Polymeric films are also perfect materials for the conjugation with biomolecules, since they can be chemically functionalized [114]. For instance, polytyramine-conducting polymer with electrochemically reduced graphene oxide (rGO) has been used for the development of new platform for detection of genomic RNA of ZIKV in human serum samples [26]. In this work, ZIKV was quantified via DPV, with LOD down to 0.1 fg/mL, showing good stability and great potential for further practical diagnostic of ZIKV ( Figure 5).…”

Section: Electrochemical Biosensors For Zikv Diagnosticmentioning

confidence: 99%

“…Different materials can improve conductive properties of biosensors and thus increase their sensitivity. Various research groups reported the possibility of functionalization of electrode surfaces by polymeric materials or used them as platforms for ZIKV detection [ 26 , 48 , 56 ]. Polymeric films are also perfect materials for the conjugation with biomolecules, since they can be chemically functionalized [ 114 ].…”

Section: Electrochemical Biosensors For Zikv Diagnosticmentioning

confidence: 99%

“…The development of progressive diagnostic tools applicable for the determination of specific pathogenic flaviviruses in biological fluids is a very promising direction. In the last few decades, the researchers have shown particular interest in the development of electrochemical biosensors for the diagnosis of DENV, ZIKV, and JEV [25][26][27]. Electrochemical biosensors measure the intensity of the electrical signal provided by a special interaction with a target analyte and proportional to its concentration.…”

Section: Introductionmentioning

confidence: 99%

“…rGO-reduced graphene oxide, PTyr-polytyramine, ZIKV-Probe-ZIKV DNA probe, gRNA-genomic RNA, DPV-differential pulse voltammetry. Adapted with permission from[26].…”

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confidence: 99%

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Label-Free Electrochemical Biosensors for the Determination of Flaviviruses: Dengue, Zika, and Japanese Encephalitis

Khristunova

Dorozhko

Короткова

et al. 2020

Sensors

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A highly effective way to improve prognosis of viral infectious diseases and to determine the outcome of infection is early, fast, simple, and efficient diagnosis of viral pathogens in biological fluids. Among a wide range of viral pathogens, Flaviviruses attract a special attention. Flavivirus genus includes more than 70 viruses, the most familiar being dengue virus (DENV), Zika virus (ZIKV), and Japanese encephalitis virus (JEV). Haemorrhagic and encephalitis diseases are the most common severe consequences of flaviviral infection. Currently, increasing attention is being paid to the development of electrochemical immunological methods for the determination of Flaviviruses. This review critically compares and evaluates recent research progress in electrochemical biosensing of DENV, ZIKV, and JEV without labelling. Specific attention is paid to comparison of detection strategies, electrode materials, and analytical characteristics. The potential of so far developed biosensors is discussed together with an outlook for further development in this field.

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Electro‐immunosensor for ultra‐sensitive determination of cardiac troponin I based on reduced graphene oxide and polytyramine

Brussasco

Guedes

Moço

et al. 2022

J of Molecular Recognition

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This work reports the construction of a novel nanostructured immunosensor for detection of the troponin I biomarker (cTnI). Anti-troponin I antibody was anchored on the modified graphite electrode with reduced graphene oxide and polytyramine for detection of troponin I in serum samples. The performance of the electro-immunosensor was evaluated by differential pulse voltammetry. The immunosensor presented a wide work range, from 4 ng mL À1 to 4 pg mL À1 , whose detection limit (4 pg mL À1 ) is significantly lower than the basal level in human serum, and maintained 100% of response after 30 days of storage. Moreover, the immunosensor showed good selectivity for detection of cTnI in real sample containing interfering substances and specificity of response to cTnI in the serum of healthy and sick patients, and demonstrated the possibility of reuse for two consecutive analyses, in addition to using a simplified and inexpensive platform when compared to other devices, demonstrating them excellent potential for application in diagnosis in the early stages of acute myocardial infarction.

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Electrochemical Detection of Zika Virus in Biological Samples: A Step for Diagnosis Point‐of‐care

Cited by 32 publications

References 38 publications

Human virus detection with graphene-based materials

Human virus detection with graphene-based materials

Label-Free Electrochemical Biosensors for the Determination of Flaviviruses: Dengue, Zika, and Japanese Encephalitis

Electro‐immunosensor for ultra‐sensitive determination of cardiac troponin I based on reduced graphene oxide and polytyramine

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