A new genosensor for meningococcal meningitis diagnosis using biological samples

Castro, Ana Cristina Honorato de; Kochi, Leandro Toshio; Moço, Anna C.R.; Coimbra, Roney Santos; Oliveira, Guilherme; Cuadros-Orellana, Sara; Madurro, João M.; Brito‐Madurro, Ana G.

doi:10.1007/s10008-018-3940-0

Cited by 14 publications

(10 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A solution of 4-AMP (2.5 mmol.L -1 ) was deaerated with ultra-pure nitrogen for 45 minutes. The electropolymerization of the monomer on the surface of the graphite electrode was performed according to literature [20]. Then, the modi ed electrode was rinsed with deionized water to remove unreacted monomers.…”

Section: Functionalization Of Graphite Surfacementioning

confidence: 99%

“…There is a great variety of materials that can be used in the construction of polymer lms, being fundamental that they allow the immobilization of active species, increase the transfer of electrons and improve the selectivity through the blocking of interferents [10]. Several electrochemical biosensors that use the genetic material for the detection of diseases can be found in the literature, for example for meningococcal meningitis [20], Epstein-Barr virus [21], the oncogenic marker MYCN [22], hepatitis C virus [23,24], Zika virus [25] and Mycobacterium leprae [26]. Srisomwat and collaborators [3] described a device based on 3D micro uidic paper (μPAD) for detecting HBV DNA.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Electrochemical Biosensor for Sensitive Detection of Hepatitis B in Human Plasma

Castro

Kochi

Flauzino

et al. 2022

Appl Biochem Biotechnol

Self Cite

View full text Add to dashboard Cite

In this work we report the construction of an electrochemical device for molecular diagnosis of hepatitis B virus in the blood plasma of infected patients, using graphite electrodes functionalized with poly(4aminophenol) and sensitized with speci c DNA probe. The recognition of genomic DNA was evaluated by electrochemical techniques (DPV and EIS) and scanning electron microscopy. The genosensor was e cient in detecting genomic DNA with a linear range from 1.176 μg.ml -1 to 4.825 μg.ml -1 and detection limit of 35.69 ng.ml -1 (4.63 UI.ml -1 or 25.93 copies.ml -1 ), which is better than 10.00 UI.ml-1 limit of reference method, real-time PCR, used in point of care. EIS analysis shows that the genosensor resistence increased exponentially with the concentration of the genomic DNA target. The developed platform has inherent advantages to its applicability in real samples, such as good sensitivity, selectivity, and low sample volume, being interesting for application in diagnosis of hepatitis B virus in blood plasma.

show abstract

Section: Functionalization Of Graphite Surfacementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Electrochemical Biosensor for Sensitive Detection of Hepatitis B in Human Plasma

Castro

Kochi

Flauzino

et al. 2022

Appl Biochem Biotechnol

Self Cite

View full text Add to dashboard Cite

show abstract

“…The current values obtained from the guanine oxidation peak remained stable after 30 minutes, indicating that it has the maximum optimum time for coating the electrode with Hep1 in these conditions. Ethidium bromide (EB) is an e cient electroactive intercalator to the DNA hybridization, due to the oxidation peak around +0.65 V [20,21], where the peak current intensity is proportional to the accumulation of genetic material on the electrode surface. This response occurs by the intercalation of BE with double-stranded DNA, which is considered the result of the interaction of a hydrophobic aromatic molecule with the hydrophobic environment of the DNA nitrogenous base pairs [28].…”

Section: Immobilization and Oligonucleotide Hybridizationmentioning

confidence: 99%

Electrochemical Biosensor for Sensitive Detection of Hepatitis B in Human Plasma

Brito‐Madurro

Castro

Kochi

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

In this work we report the construction of an electrochemical device for molecular diagnosis of hepatitis B virus in the blood plasma of infected patients, using graphite electrodes functionalized with poly(4-aminophenol) and sensitized with specific DNA probe. The recognition of genomic DNA was evaluated by electrochemical techniques (DPV and EIS) and scanning electron microscopy. The genosensor was efficient in detecting genomic DNA with a linear range from 1.176 μg.ml-1 to 4.825 μg.ml-1 and detection limit of 35.69 ng.ml-1 (4.63 UI.ml-1 or 25.93 copies.ml-1), which is better than 10.00 UI.ml-1 limit of reference method, real- time PCR, used in point of care. EIS analysis shows that the genosensor resistence increased exponentially with the concentration of the genomic DNA target. The developed platform has inherent advantages to its applicability in real samples, such as good sensitivity, selectivity, and low sample volume, being interesting for application in diagnosis of hepatitis B virus in blood plasma.

show abstract

“…Ethidium bromide (EtBr) was utilized as an electroactive label to investigate hybridization. 710‐bp amplicon as complementary sequences and bacterial genomes were identified using a DPV technique and a detection limit of 0.6 ng/μl was obtained 110 …”

Section: Biosensing Based On Type Of Bacteriamentioning

confidence: 99%

“…For complete of discussion, Table 2 25‐206 summarized all of the discussed articles on the detection of bacterial. It is important to point out that, there is other reports in this research area …”

Section: Biosensing Based On Type Of Bacteriamentioning

confidence: 99%

Recent progress and challenges on the bioassay of pathogenic bacteria

2020

View full text Add to dashboard Cite

The present review (containing 242 references) illustrates the importance and application of optical and electrochemical methods as well as their performance improvement using various methods for the detection of pathogenic bacteria. The application of advanced nanomaterials including hyper branched nanopolymers, carbon‐based materials and silver, gold and so on. nanoparticles for biosensing of pathogenic bacteria was also investigated. In addition, a summary of the applications of nanoparticle‐based electrochemical biosensors for the identification of pathogenic bacteria has been provided and their advantages, detriments and future development capabilities was argued. Therefore, the main focus in the present review is to investigate the role of nanomaterials in the development of biosensors for the detection of pathogenic bacteria. In addition, type of nanoparticles, analytes, methods of detection and injection, sensitivity, matrix and method of tagging are also argued in detail. As a result, we have collected electrochemical and optical biosensors designed to detect pathogenic bacteria, and argued outstanding features, research opportunities, potential and prospects for their development, according to recently published research articles.

show abstract

A new genosensor for meningococcal meningitis diagnosis using biological samples

Cited by 14 publications

References 34 publications

Electrochemical Biosensor for Sensitive Detection of Hepatitis B in Human Plasma

Electrochemical Biosensor for Sensitive Detection of Hepatitis B in Human Plasma

Electrochemical Biosensor for Sensitive Detection of Hepatitis B in Human Plasma

Recent progress and challenges on the bioassay of pathogenic bacteria

Contact Info

Product

Resources

About