Sugar cane is widely used in an in natura forage in tropical countries, but the adoption of silage methods facilitates the preservation of its nutritional value and improves the logistics of its use. To explain differences in performance using alternative forages, it is important to conduct studies that evaluate the various digestion sites for the nutrients provided in diets. However, considering that the collection of omasal digesta is quite laborious and requires the use of a vacuum pump, reticular sampling has been suggested as a promising alternative. Thus, the objective of this study was to evaluate the intake and ruminal digestibility obtained from samples of digesta collected in the reticulum and omasum of cattle fed different diets. Five rumen-fistulated crossbred cattle with an average initial live weight of 3367 16.6 kg were used, being distributed in a 5 Â 5 Latin square design. Five diets were evaluated, which contained 60% forage and 40% concentrate on dry matter basis using different forages: maize silage (CS); sugar cane in natura (SCIN); sugar cane silage (SCS0%); sugar cane silage treated with 0.4% calcium oxide (SCS0.4%) or 0.8% calcium oxide (SCS0.8%) on wet basis. The percentage of crude protein (CP) in all of the forages was corrected to 11% based on dry matter (DM) using a mixture of urea/ ammonium sulfate (9:1). Six collections of reticular and omasal digesta were obtained over three days at 12 h intervals. To calculate the flow of reticular and omasal nutrients, a double marker system was employed, using cobalt-EDTA and indigestible neutral detergent fiber (NDFi) as markers. The reticular and omasal digesta were similar (P 40.05) to estimate ruminal digestibility of DM, organic matter (OM), CP, neutral detergent fiber (NDF) and non-fiber carbohydrates (NFC). However, the ruminal digestibility of ether extract (EE) and the intestinal digestibility of CP and EE differed (P o 0.05) between sampling sites. The results indicate that the omasal digesta is more suitable than the reticular digesta for measuring the ruminal digestion of diet components.
Fecal production and apparent dry matter digestibility (ADMD) were evaluated using external markers (chromium oxide; titanium dioxide; isolated, purified, and enriched lignin (LIPE®); and isolated, purified, and enriched lignin nanoparticles (NANOLIPE®) and internal markers (indigestible DM (IDM), indigestible neutral detergent fiber (INDF), and indigestible acid detergent fiber (IADF) in diets based on Tifton 85 bermuda grass (Cynodon sp.) hay containing different concentrations of a cocoa by-product. Sixteen crossbred (Holstein × Zebu) dairy heifers with a mean live weight of 363.00 ± 27.70 kg were evaluated and distributed in a completely randomized block design with a split-plot arrangement. The plots corresponded to the diets, which differed in the substitution of bermuda grass hay with different concentrations (0, 8, 16, and 24% of DM) of the cocoa by-product, whereas the splitplots represented the indigestible markers. Chromic oxide, LIPE®, NANOLIPE®, and INDF accurately estimated ADMD and fecal production whereas titanium dioxide, IDM, and IADF did not accurately estimate these parameters.
The aim of this study was to determine the nutritional requirements of energy and protein for maintenance and weight gain of crossbred cattle, as well as their efficiencies. Fifty ¾ Zebu × ¼ Holstein crossbred bulls with initial weights of 214±4kg and aged 11±0.2 months on average were used in this experiment. Four animals were used in the reference group; ten bulls were fed at the maintenance level; and the remaining 36 bulls were fed ad libitum and distributed in a completely randomized design in a 3×3 factorial arrangement, which had three feedlot periods (56, 112 or 168 days) and three calcium and phosphorus levels (low, medium and normal) in the diet. Four of the maintenance animals had their heat production measured by respirometry at the Laboratory of Metabolism and Calorimetry of UFMG. After slaughter, composite samples, referred to as carcass and noncarcass samples were obtained from each animal. The net energy requirements for maintenance (NE m ) and metabolizable energy for maintenance (ME m ) were 68.9 and 90.1 kcal/EBW 0.75 /day, respectively. The efficiency (k m ) was 76.41%. The NE m requirement determined in the respirometry chamber was 85.5 kcal/kg 0.75 . The following equations were obtained for net energy for gain (NE g ) and net protein for gain (NP g
Forty 3/4 Bos indicus · 1/4 Bos taurus bulls (initial bodyweight = 214 AE 4 kg; 11 AE 0.2 months) were used in the study. Four bulls were slaughtered as reference and the other 36 bulls were allowed ad libitum consumption of treatment diets following a completely randomised 3 · 3 factorial design. The design included three diet concentrations of calcium (Ca) and phosphorus (P): standard, 50% of standard, and 75% of standard and three periods of feedlot confinement: 8, 16, and 24 weeks with four bulls per treatment combination. The diets were isonitrogenous and consisted of 60 : 40 corn silage to concentrate. Dry matter intake, crude protein, ether extract, organic matter, non-fibrous carbohydrates, Ca, and P, expressed as kg/day, were greater (P < 0.05) for bulls confined for longer periods. Nutrient digestibility and average daily gain was not affected (P > 0.05) by Ca and P concentration of diet or by feedlot phase. Dressing were decreased (P < 0.05) for bulls that remained in confinement for a shorter period. The daily maintenance requirements of Ca and P were 13.66 and 21.51 mg/kg empty bodyweight, respectively, and the absorption coefficients of Ca and P were 0.70 and 0.67, respectively. We concluded that for crossbred cattle in the feedlot, dietary levels of Ca and P recommended by BR-CORTE (2010), NRC (2000, and AFRC (1991) could be reduced by 62%, 66%, and 57%, respectively, for Ca and by 14%, 15%, and 43%, respectively, for P with significant savings in costs. This study shows that Ca and P can be reduced to 1.8 and 2.2 g/kg DM respectively, in the diets for crossbred cattle in feedlot.We hypothesised that the dietary requirements of Ca and P, as described by BR-CORTE (2010), can be reduced without affecting the performance of beef cattle in feedlot.The objective of this study was to evaluate nutrient intake and digestibility, performance, maintenance requirements, and absorption coefficients for Ca and P in crossbred bulls fed diets with three different concentrations of Ca and P within three different feedlot phases.
This study was undertaken to examine the intake, nutrient digestibility, protein and energy balance and feeding behavior of dairy heifers fed diets in which Tifton 85 bermuda grass (Cynodon sp) hay was substituted by a passion fruit (Passiflora edulis) by-product. Sixteen Holstein × Zebu crossbred heifers with an average weight of 363.00 ± 27.7 kg were assigned to four treatments represented by diets in which levels of a passion fruit by-product (0, 12, 24 and 36%; dry matter basis) were added as a substitute for Tifton 85 bermuda grass. The intakes of crude protein and ether extract (P > 0.05) rose as the by-product was added to the diets. Nitrogen intake, absorbed nitrogen and urinary nitrogen also increased with the inclusion of the by-product. Passion fruit by-product can be included in diets for dairy heifers up to the level of 36%.
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