Twenty-four multiparous dairy cows (eight with ruminal cannulae) were blocked by days in milk and assigned to six balanced 4 x 4 Latin squares with 21-d periods. The four diets, formulated from alfalfa silage plus a concentrate mix based on ground high moisture ear corn, contained (dry matter basis): 1) 20% concentrate, 80% alfalfa silage (24% nonfiber carbohydrate; NFC), 2) 35% concentrate, 65% alfalfa silage (30% NFC), 3) 50% concentrate, 50% alfalfa silage (37% NFC), or 4) 65% concentrate, 35% alfalfa silage (43% NFC). Soybean meal and urea were added to make diets isonitrogenous with equal nonprotein nitrogen (NPN) (43% of total N). Total urine was collected with indwelling Folley catheters for 24 h during each period. There was no effect of diet on urinary creatinine excretion (average 29 mg/kg of BW/d). There were quadratic effects of diet on total urinary ecretion of allantoin, uric acid, and purine derivatives (allantoin plus uric acid), and on ruminal synthesis of microbial N estimated from purine derivatives; maxima occurred at about 35% dietary NFC. Urinary excretion also was estimated with spot urine samples from creatinine concentration and the mean daily creatinine excretion. Daily excretion of allantoin, uric acid, and purine derivatives estimated from spot urine sampling followed the same pattern as that observed with total collection; differences between measured and estimated urine volume were significant only for 35% dietary concentrate. Spot urine sampling appeared to yield satisfactory estimates of purine derivative excretion. Maximal urea N excretion was estimated to occur at about 31% dietary NFC. Milk allantoin secretion increased linearly with concentrate and accounted for 4 to 6% of the total purine derivative excretion. Microbial yield was maximal at 35% dietary NFC, suggesting that this was the optimal level for utilization of dietary NPN from alfalfa silage and other sources.

RESUMOAvaliaram-se as dinâmicas de trânsito e degradação da fibra em detergente neutro (FDN) em bovinos alimentados com forragem tropical de baixa qualidade e compostos nitrogenados. Foram utilizadas cinco novilhas Holandês x Zebu fistuladas no rúmen. A alimentação volumosa basal dos animais foi constituída por feno de capim-braquiária (Brachiaria decumbens Stapf.), com 5,08% de proteína bruta (PB), com base na matéria seca (MS), fornecido ad libitum. Os cinco tratamentos avaliados foram definidos de acordo com o nível de suplementação proteica (0, 3, 5, 7 e 9 pontos percentuais acima do nível de PB da forragem). Como fonte de compostos nitrogenados, empregou-se mistura de ureia:sulfato de amônia:albumina (4,5:0,5:1,0). O experimento foi estruturado segundo delineamento em quadrado latino 5 x 5. Os níveis médios de PB nas dietas foram de 5,28; 8,08; 9,82; 11,87 e 13,63%, com base na MS. Verificou-se elevação linear (P<0,05) da fração potencialmente degradável da FDN até o nível de 8,62% de PB, com platô estimado de 47,92% da FDN. Verificou-se efeito quadrático (P<0,05) sobre a taxa de degradação da fração potencialmente degradável da FDN em função dos níveis de PB da dieta, com máxima resposta estimada sobre 13,39% de PB. O fluxo ruminal de partículas fibrosas apresentou relação linear-response-plateau em função do nível de PB na dieta (P<0,05), com ponto crítico para o início do platô sobre 7,59% de PB.Palavras-chave: Brachiaria decumbens, repleção ruminal, suplementação, taxa de degradação, taxa de passagem (0, 3, 5, 7, and 9 percentile points above the CP level of the roughage). The supplement was a mixture of urea, ammonium sulfate, and albumin (4.5:0.5:1.0, respectively). The experiment was carried out according to a 5 x 5 Latin square design. The average CP levels in the diets were: 5.28, 8.08, 9.82, 11.87, and 13.63% in DM basis. The potentially degradable Recebido em 11 de setembro de ABSTRACT Rumen transit and degradation dynamics of neutral detergent fiber (NDF) in cattle fed

Twenty-four multiparous lactating Holstein cows were blocked by days in milk and assigned to treatment sequences in a replicated 4x4 Latin square with 21-d periods. The four diets, formulated from alfalfa silage plus a concentrate mix based on ground high moisture ear corn, contained [dry matter (DM) basis]: 1) 20% concentrate, 80% alfalfa silage (24% nonfiber carbohydrates; NFC), 2) 35% concentrate, 65% alfalfa silage (30% NFC), 3) 50% concentrate, 50% alfalfa silage (37% NFC), or 4) 65% concentrate, 35% alfalfa silage (43% NFC). Soybean meal and urea were added to make diets isonitrogenous with equal nonprotein N (43% of total N). Intake of DM and milk yield indicated that adaptation was complete within 7 d of changing the diets within the Latin square. There were linear increases in apparent digestibility of DM and organic matter, and a linear decrease in neutral detergent fiber (NDF) digestibility with increasing dietary NFC. Solutions of significant quadratic equations yielded estimated maxima for intake of DM, organic matter, digestible organic matter, and NDF at, respectively, 37, 38, 43, and 27% dietary NFC. There were linear increases in yields of milk, protein, lactose, and solids not fat with increasing dietary NFC. Feed efficiency (milk/DM intake) yielded a quadratic response with a minimum at 27% dietary NFC. Maxima for milk fat content, fat yield, and fat-corrected milk yield were estimated to occur at, respectively, 30, 34 and 38% dietary NFC. In this short-term trial, maximal DM intake and fat-corrected milk yield indicated that the optimum concentrate for cows fed high moisture ear corn plus alfalfa silage as the only forage was equivalent to 37 to 38% dietary NFC; however, yields of milk, protein and solids not fat were still increasing at 65% dietary concentrate (43% NFC).

Thirty non-castrated male lambs with 20±2.3 kg average body weight (BW) were randomly assigned to five treatments consisted of different dietary concentrations of crude glycerin (CG; 0, 3, 6, 9 and 12% on DM basis) to evaluate the effects on performance, carcass and meat quality traits. A quadratic effect was observed for performance (P=0.04), final BW (P<0.01) and hot carcass weight (P<0.01). No effects of CG were observed (P>0.05) on carcass pH neither on shear-force, cooking loss and ether extract content in longissimus. The inclusion of CG tended to reduce the Zn content in meat (P=0.09). The data suggests that CG (36.2% of glycerol and 46.5% of crude fat) may be used in diets of finishing lambs with concentrations up to 3% without negative effects on performance and main carcass traits. Moreover, inclusion of CG seems to not affect quality and safety of meat for human consumption.

Twenty-six comparative slaughter studies were used (n = 752 animals) and coded within each experiment by gender (431 bulls, 204 steers, and 117 heifers) and breed (447 Nellore and 305 Bos indicus and Bos taurus crossbreds) to develop equations to predict the efficiency of use of ME to NE for growth (kg) and ME to NE for maintenance (km). The retained energy (RE) was regressed on ME intake (MEI) available for gain using orthogonal regression to obtain the kg within each experiment. The estimated kg was regressed on RE as protein (REp) according to the following equation: kg = a/(b + REp). Gender and breed effects were not tested because of limited number of experiments. The km was estimated as the intercept of the following equation: HP = β0 × e((β1 × MEI)), in which HP is heat production, β0 and β1 are coefficients, and e is the natural logarithm. The ME for maintenance (MEm) was computed assuming MEI equals to HP at maintenance. The km was obtained using the stepwise procedure of a multiple regression including ADG, empty body gain (EBG), empty BW (EBW), EBW(0.75), kg, and energy content in the EBW. A random coefficient model, assuming a random variation for study effects, was used to test breed and gender effects to identify the best model to estimate km. The overall equation to predict kg was 0.327 (±0.142)/[0.539 (±0.317) + REp], with an R(2) of 0.963. The equation to predict km was 0.513 (±0.024) + 0.173 (±0.061) × kg + a × EBG, R(2) = 0.92, in which a = 0.100 (±0.021) for B. indicus or a = 0.073 (±0.021) for crossbreds. Our results indicated that B. indicus were more efficient to use ME for maintenance. We concluded that km can be predicted from kg and EBG and that B. indicus × B. taurus crossbreds can affect km. Furthermore, kg can be predicted from REp and neither gender nor crossbreeding (B. indicus × B. taurus) affected kg. Because our database consisted of Nellore and B. indicus and B. taurus crossbreds, it is necessary to further evaluate differences between B. taurus and B. indicus regarding the kg.

O objetivo deste trabalho foi avaliar o comportamento ingestivo de novilhas mestiças Holandês x Zebu alimentadas com dietas à base de cana-de-açúcar suplementadas com fontes de nitrogênio não-protéico [NNP] (uréia e cama de frango, em substituição parcial da uréia) e, ou, adição de probióticos (sem probiótico, com levedura ou com microbiota ruminal). Vinte e quatro novilhas, com idade e peso médio inicial de 15 meses e 247 kg, respectivamente, foram mantidas em baias individuais e alimentadas à vontade, durante o período experimental de 84 dias. Os animais foram distribuídos em delineamento inteiramente casualisado, em esquema fatorial 2 x 3 (fontes de NNP vs fontes de probiótico), com quatro repetições. O comportamento ingestivo de cada novilha foi determinado visualmente, a cada 28 dias, a intervalos de 10 minutos, durante 24 horas. Não houve diferença no tempo despendido em alimentação, ruminação e mastigação total, em min/dia, das novilhas alimentadas com diferentes fontes de NNP e probióticos. O tempo médio de ruminação, em min/kg de MS e FDN, foi maior para os animais alimentados com uréia. O comportamento ingestivo das novilhas não foi influenciado pelas fontes de nitrogênio ou pela adição de probiótico.

Enteric methane (CH 4 ) production attributable to beef cattle contributes to global greenhouse gas emissions. Reliably estimating this contribution requires extensive CH 4 emission data from beef cattle under different management conditions worldwide. The objectives were to: 1) predict CH 4 production (g d −1 animal −1 ), yield [g (kg dry matter intake; DMI)−1 ] and intensity [g (kg average daily gain) −1 ] using an intercontinental database (data from Europe, North America, Brazil, Australia and South Korea); 2) assess the impact of geographic region, and of higher-and lower-forage diets. Linear models were developed by incrementally adding covariates. A K-fold cross-validation indicated that a CH 4 production equation using only DMI that was fitted to all available data had a root mean square prediction error (RMSPE; % of observed mean) of 31.2%. Subsets containing data with

Effects of supplemental RDP and RUP on nutrient digestion, N metabolism, urea kinetics, and muscle protein degradation were evaluated in Nellore heifers (Bos indicus) consuming low-quality signal grass hay (5% CP and 80% NDF, DM basis). Five ruminally and abomasally cannulated Nellore heifers (248 ± 9 kg) were used in a 5 × 5 Latin square. Treatments were the control (no supplement) and RDP supplementation to meet 100% of the RDP requirement plus RUP provision to supply 0, 50, 100, or 150% of the RUP requirement. Supplemental RDP (casein plus NPN) was ruminally dosed twice daily, and RUP supply (casein) was continuously infused abomasally. Jugular infusion of [ 15 N 15 N]-urea with measurement of enrichment in urine was used to evaluate urea kinetics. The ratio of urinary 3-methylhistidine to creatinine was used to estimate skeletal muscle protein degradation. Forage NDF intake (2.48 kg/d) was not affected (P ≥ 0.37) by supplementation, but supplementation did increase ruminal NDF digestion (P < 0.01). Total N intake (by design) and N retention increased (P < 0.001) with supplementation and also linearly increased with RUP provision. Urea entry rate and gastrointestinal entry rate of urea were increased by supplementation (P < 0.001). Supplementation with RUP linearly increased (P = 0.02) urea entry rate and tended (P = 0.07) to linearly increase gastrointestinal entry rate of urea. Urea use for anabolic purposes tended (P = 0.07) to be increased by supplementation, and RUP provision also tended (P = 0.08) to linearly increase the amount of urea used for anabolism. The fraction of recycled urea N incorporated into microbial N was greater (P < 0.001) for control (22%) than for supplemented (9%) heifers. Urinary 3-methylhistidine:creatinine of control heifers was more than double that of supplemented heifers (P < 0.001). Control heifers reabsorbed a greater (P < 0.001) fraction of urea from the renal tubule than did supplemented heifers. Overall, unsupplemented heifers had greater mobilization of AA from myofibrillar protein, which provided N for urea synthesis and subsequent recycling. Supplemental RUP, when RDP was supplied, not only increased N retention but also supported increased urea N recycling and increased ruminal microbial protein synthesis.