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Background: Wound infection can cause delayed healing, chronicity which indirectly causes financial burden and psychological stress on patients. Aim: To speciate the organism isolated from pus sample received and its antibiotic sensitivity test. Methods and materials: All isolated organism are identified by colony morphology, Gram stain and biochemical reactions. Antibiotic sensitivity test for all isolates were done by Kirby-Bauer method using Mueller Hinton agar. Results: The major contribution of sample was from surgery department (65.3%) followed by orthopedics (10.2%). A total of 383 organism isolated among which Klebsiella pneumoniae dominates (34.46%) followed by Staphylococcus aureus (18.53%). All Gram negative organisms showed maximum resistance to amoxyclav and least to Imipenem. All Gram positive organisms showed least resistance to Vancomycin and Linezolid. Pseudomonas aeruginosa showed maximum resistance to amoxyclav (66.1%) and Gentamicin (57.1%) and least to Imipenem (7.1%). Conclusion: It is observed from the present study that, there is an increase in the resistance among beta-lactam antibiotics and quinolones. Emergence of drug resistance can be effectively controlled by continuous surveillance in hospitals and rational use of antibiotics.

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Aim: To find the prevalence of MBL Producing Pseudomonas aeruginosa from various clinical specimen. Material and Methods: A total 114 cases from which Pseudomonas aeruginosa has been isolated from Swab, Urine, pus, Sputum, Bal, Foley's catheter, E.T. Secretion received from various clinical departments. The study was carried outovera period of Six months. The isolates were tested by IPM-EDTA Combined Disc Test (CDT) and Imipenem-EDTA double disc synergy test (DDST). Descriptive statistics and chi-square is used with the help of MS Excel and SPSS Version 25 Results: Among 114 Pseudomonas aeruginosa isolated from various clinical specimens, 16 (14.03%) imipenemresistant Pseudomonas isolates. Out of 16 IPM resistant, 15(93.75%) were positive for MBL by CDT-IPM method and 11 (68.75%) were positive by Imipenem-EDTA (DDST) method, respectively. Conclusion: Increase in the resistant pattern of antibiotics can lead to increased morbidity, mortality and economic burden on patients. So it is necessary to detect MBL producing Pseudomonasaeruginosa by simple and effective methods.

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Aims of the study are to detect biofilm producing Staphylococcus epidermidis isolated from various clinical specimens. Total 73 Staphylococcus epidermidis isolates were collected from clinical samples like blood, post-operative wound swabs, IV catheter tips, catheterized urine, and exudates received from various clinical departments. The study was carried out over a period of one year. The specimens received were processed by conventional methods. Tissue Culture plate method was used for detection of biofilm. IV catheter tip samples revealed 25%, implant device associated infections revealed 20%, the Catheterized urine samples showed 17%, blood culture 6%, ventilator associated infections 20%, post-operative wound infections 13.29% and exudates 3.33% of Staphylococcus epidermidis isolates. Isolates with O. D. values more than 0.2 were considered as high biofilm producers. 52.1% of S. epidermidis isolates were weak biofilm producers, 24.66% were moderate biofilm producers and 20.54% were high biofilm producers. Isolates from IV catheter tips showed high biofilm formation. Increase in use of implant devices, unnecessary and prolonged use of urinary catheter and IV catheters can lead to biofilm formation which pose difficulty in treating and eradicating them.