Characterization and resistance pattern of bacterial isolates from pus samples in a tertiary care hospital, Karimnagar

Sirigadha, Krishnamurthy; Sajjan, Amar C.; Swetha, Swetha; Srivastav, Shalini

doi:10.17511/jopm.2016.i02.03

Cited by 4 publications

(1 citation statement)

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“…Pyrogenic infections that are characterised by local and systemic inflammations can be either due to single organism or multiple pathogens. The study revealed that 84.2% (112 samples) were positive for the isolation of pathogens from pus samples that was higher compared to the reports of [18]. The aetiological agents include Gram positive and Gram negative bacteria like Staphylococcus aureus, Enterococci, Pseudomonas aeruginosa, Escherichia coli, Klebsiella sps and Proteus sps.…”

Section: Discussionmentioning

confidence: 68%

A Retrospective Study on the Pyogenic Pathogens and Their Antibiotic Susceptibility Patterns along with the ES<i>β</i>L Production

Chakraborty¹,

Chanda²,

Choudhury³

et al. 2021

AiM

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Pyogenic infections are caused by various pathogens leading to pus formation and that can be attributed due to a wound either through accident or during surgery leading to infection spread. There are pathogenic strains that are not uncommon in hospital settings like Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter etc., that are multidrug resistant (MDR) and are a cause of concern. The bacteriological profile in the pyogenic infections tends to be same but there is a vast difference with the antibiotic resistant patterns in different hospital settings. Hence, the aim was to study the antibiotic susceptibility profiles and Extended spectrum βeta Lactamases (ESβL) production in these pathogens. A prospective study was carried out in Silchar Medical College and Hospital Assam, India, over a four-month period from February to May 2021. The samples were processed using Blood and MacConkey's agar. Further, these isolated pathogens were identified by standard morphological, cultural and biochemical tests. The antibiotic susceptibility test was conducted by Kirby Bauer disc diffusion method and ESβL production was detected by using combined disk diffusion test. It was observed that the identified pathogens had an incidence rate of 84.2% and further revealed that Gram negative had a higher incidence rate compared to Gram positive with 59.8%. The pathogens isolated from pus samples had a maximum of Klebsiella sps (19.64%) and the lowest was E. coli with 5.36%. Antibiotic susceptibility test (AST) of Gram-negative bacterial isolates showed the highest incidence with aztreonam (40.6%) and the lowest was observed in Piperacillin/Tazobactam with 7.5%. The only Gram positive

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Section: Discussionmentioning

confidence: 68%

A Retrospective Study on the Pyogenic Pathogens and Their Antibiotic Susceptibility Patterns along with the ES<i>β</i>L Production

Chakraborty¹,

Chanda²,

Choudhury³

et al. 2021

AiM

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Bacteriological profile of pus samples and their antibiotic susceptibility pattern

Wadekar¹,

Sathish²,

Jayashree³

et al. 2020

IJMR

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Pyogenic infections can be caused by various microorganisms and mixed infections are common which require antibiotic therapy. The inappropriate use of antibiotics has resulted in development of antibiotic resistance. The bacteriological profile may remain same, but antibiotic susceptibility pattern varies. Hence the study was conducted to know bacteriological profile of pus samples and their antibiotic susceptibility pattern. Materials and Methods: A retrospective study was carried out from January to June 2019. 108 pus samples collected during study period were included. The samples were cultured on Blood and MacConkey agar. After aerobic incubation at 37 o C for 18-24 hrs, organisms were identified by standard methods and antibiotic susceptibility was tested by Kirby Bauer disc diffusion method. ESBL was detected by combined disk test and Methicillin resistant Staphylococcus aureus (MRSA) by Cefoxitin 30 µg disc. Results: Of 118 pus samples collected, 101(85.5%) were positive cultures and no growth was in 17(14.4%) samples. S. aureus 27(22.9%) was most common Gram positive isolate and Pseudomonas spp. 17(14.4%), was most common gram negative isolate. ESBL positivity was seen in 38(61.2%) and MRSA in 13(48.1%) S. aureus isolates. Most of gram negative isolates were susceptible to piperacillin / tazobactum 55(88.7%) and meropenem 53(85.4%) and gram positive isolates to vancomycin 27(100%) and linezolid 25(92.5%). Conclusion:The spread of beta-lactamase producing organisms has been increasing. Our study showed increased resistance to beta-lactam antibiotics which is a serious problem. To combat resistance irrational use of antibiotics should be avoided. Also regular surveillance helps in implementing better therapeutic strategies to reduce morbidity and mortality.

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Antibiotic susceptibility trends in bacterial isolates from wound infections

Sheeba,

Prathyusha,

Anila

2024

Microbiology Independent Research Journal (MIR Journal)

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Pyogenic infections leading to pus formation are associated with various inflammatory mediators and the proliferation of microbial flora. Wound infections can be caused by both aerobic and anaerobic bacteria, viruses, fungi, or even protozoans. Timely treatment of infected patients is critical since untreated or poorly treated pyogenic infections can lead to mortality or severe complications. Successful treatment is defined by choosing the right antibiotic, which is hindered by the spreading of bacterial drug resistance. It is well documented that overuse of antimicrobials before and especially during the COVID-19 pandemic has resulted in the emergence of drug resistance among various microorganisms. Our study aimed to identify common etiological agents associated with wound infections and to evaluate their antibiotic resistance patterns. A cross-sectional study was conducted by analyzing the antibiograms of different aerobic bacteria isolated from wound samples of patients from the Palakkad District Hospital in Kerala, India, from 2017 through 2019. All variables, including age, sex, bacterial isolate, and antibiogram, were tabulated and the chi-square test was used for analysis. Out of the 1,269 bacterial isolates analyzed, the most common gram-negative strain was Escherichia coli (23%) followed by Pseudomonas aeruginosa (20%). Among the gram-positive isolates, Staphylococcus aureus was the most common (19%). We found only 15% of Staphylococcus aureus isolates were methicillin-resistant, against the national average of 41%. The most resistant strain in our study was Acinetobacter baumannii. Statistically significant changes in antibiotic resistance patterns were observed over the three years of the study period.

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Characterization and resistance pattern of bacterial isolates from pus samples in a tertiary care hospital, Karimnagar

Cited by 4 publications

References 4 publications

A Retrospective Study on the Pyogenic Pathogens and Their Antibiotic Susceptibility Patterns along with the ES<i>β</i>L Production

A Retrospective Study on the Pyogenic Pathogens and Their Antibiotic Susceptibility Patterns along with the ES<i>β</i>L Production

Bacteriological profile of pus samples and their antibiotic susceptibility pattern

Antibiotic susceptibility trends in bacterial isolates from wound infections

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Characterization and resistance pattern of bacterial isolates from pus samples in a tertiary care hospital, Karimnagar

Cited by 4 publications

References 4 publications

A Retrospective Study on the Pyogenic Pathogens and Their Antibiotic Susceptibility Patterns along with the ES&lt;i&gt;β&lt;/i&gt;L Production

A Retrospective Study on the Pyogenic Pathogens and Their Antibiotic Susceptibility Patterns along with the ES&lt;i&gt;β&lt;/i&gt;L Production

Bacteriological profile of pus samples and their antibiotic susceptibility pattern

Antibiotic susceptibility trends in bacterial isolates from wound infections

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Resources

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A Retrospective Study on the Pyogenic Pathogens and Their Antibiotic Susceptibility Patterns along with the ES<i>β</i>L Production

A Retrospective Study on the Pyogenic Pathogens and Their Antibiotic Susceptibility Patterns along with the ES<i>β</i>L Production