Prevalence and Antifungal Susceptibility of Candida Species Isolated From Patients Attending Tertiary Care Hospital

Sajjan, Amar C.; Mahalakshmi, V. V.; Hajare, Drvinay

doi:10.9790/0853-13524449

Cited by 11 publications

(13 citation statements)

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“…The most predominant species was found to be C. albicans (45.5%), followed by C. tropicalis (28.88%), C. krusei (20%), C. glabrata (3.33%), and C. parapsilosis (2.22%), respectively. Our findings are similar to the previously reported data by Sajjan et al [15]. Zarei-Mahmoudabadi et al and Chakrabarti et al, however, reported a lower prevalence rate (39% and 25%) of C. albicans [16, 17].…”

Section: Discussionsupporting

confidence: 93%

“…The rate of isolation of Candida spp. in females was about 61.2%, while in males, it was only 38% [15]. In the present study, the distribution of Candida spp.…”

Section: Discussionsupporting

confidence: 41%

“…was from urine (43%) followed by BAL/Sputum (18.88%), high vaginal swab (8.88%), central line/ET/oral suction tip (7.77%), blood and wound swabs (6.66%), pus (3.33%), bile aspirate (2.22%), and deep tissue and skin scrapping (1.11%), respectively. However, the study done by Saijan et al (2014) showed the highest recovery of Candida spp from high vaginal swabs [15]. This could be because, in our study, we have processed more number of urine samples than the high vaginal swabs.…”

Section: Discussionmentioning

confidence: 82%

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Antifungal Susceptibility and Biofilm Production of Candida spp. Isolated from Clinical Samples

Marak

Dhanashree

2018

International Journal of Microbiology

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Objective The study aims to speciate clinical Candida isolates and detect their biofilm-forming ability and antifungal resistance. Methods All the Candida spp. isolated from different clinical samples like pus, urine, blood, and body fluid were included in the study. Biofilm production was tested by the microtiter plate method. Antifungal susceptibility was studied by the disk diffusion method. Patient's demographic details such as age, sex, and clinical information were collected. Presence of other risk factors such as diabetes mellitus, history of antibiotic use, and any urinary tract instrumentations was also recorded. Results Among 90 Candida species isolated, most predominant species was found to be C. albicans (45.5%) followed by C. tropicalis (28.88%), C. krusei (20%), C. glabrata (3.33%), and C. parapsilosis (2.22%). Candida spp. were isolated from urine (43%), BAL/sputum (18.88%), high vaginal swab (8.88%), suction tips (7.77%), blood and wound swabs (6.66%), pus (3.33%), bile aspirate (2.22%), and deep tissue (1.11%). A larger number of females were affected than males, and the age group of 51 to 60 years was more susceptible to candidiasis. A higher number of C. albicans isolates produced biofilm followed by C. parapsilosis, C. tropicalis, and C. krusei. However, C. glabrata showed no biofilm production in our study. All Candida isolates were 100% sensitive to amphotericin B. Voriconazole was the next effective drug with 81.11% susceptibility. 24.44% of strains were resistant to fluconazole. Conclusion Speciation of Candida isolates, detection of ability to form the biofilm, and monitoring of antifungal susceptibility testing are necessary for appropriate treatment.

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Section: Discussionsupporting

confidence: 93%

“…The rate of isolation of Candida spp. in females was about 61.2%, while in males, it was only 38% [15]. In the present study, the distribution of Candida spp.…”

Section: Discussionsupporting

confidence: 41%

Section: Discussionmentioning

confidence: 82%

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Antifungal Susceptibility and Biofilm Production of Candida spp. Isolated from Clinical Samples

Marak

Dhanashree

2018

International Journal of Microbiology

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“…Also, in various countries, significant geographic variations in the etiological pattern of invasive Candida species is reported [13]. In the present study, commonest NAC species isolated was C. tropicalis comparable to other studies [14,15]. Among the lower respiratory tract samples, sputum samples grew C. albicans (85%), C. tropicalis (11%) and C. glabrata (4%) and one endotracheal aspirate grew C. albicans with significant colony count.…”

Section: Discussionsupporting

confidence: 85%

Speciation and antifungal susceptibility testing of Candida isolates in various clinical samples in a tertiary care hospital in Mumbai

Jangla

Naidu

Patel

2018

Int Jour of Biomed Res

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Background: Over the last few years fungal infection rates have increased and a change is seen in their epidemiology and antifungal susceptibility pattern. Hence this study was conducted to learn the distribution of Candida species in various samples and their antifungal susceptibility pattern.Material and Methods: A total of 60Candida isolates were included in the study. Identification was done by colony morphology and Gram stain. Speciation was carried out by Germ-tube test, urease test, chlamydoconidia production test, colony characteristics on chromogenic agar medium, sugar assimilation test,sugar fermentation testand Vitek2 compact(Biomeriux, France) using ID-YST cards. Antifungal testing was done on Vitek2 compact using AST YS01 cards which included fluconazole, voriconazole, amphotericin b, caspofungin, micafungin and flucytosine.Results: 60 Candida isolates were included in this study. Samples from which Candida species were isolated were sputum (45%), urine (33.5%), pus (12%), vaginal swab (5%), endotracheal secretion (1.5%), blood (1.5%) and tissue (1.5%). Isolates from males and females were 30% and 70% respectively. Isolates from geriatric age group (>65 years) and adults (18-65 years) were 52% and 48% respectively. Isolates from samples received from IPD, OPD and ICU were 58%, 34% and 8% respectively. Out of all isolates, Candida albicans was 58%, Candida tropicalis 20%, Candida glabrata 10%, Candida parapsilosis 9% and Candida krusei3%. All Candida species (except Candidaglabrata) showed 100% sensitivity to amphotericin b and caspofungin. Sensitivity to azole group of drugs was 100% among NAC except C. glabrata and C. krusei and more than 90% among C. albicans.Conclusion: Candida albicans was the commonest isolate followed by C. tropicalis among the NAC. Overall also, C. Albicans were predominant as compared to Non albicans Candida (NAC) species. All Candidaisolates except (C. glabrata) showed good sensitivity to all antifungals.

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“…Azoles are considered as the first level of management in Candida infection but there is increase in the resistance [ 1 ]. Since C. albicans is the common fungal causative agent in superficial and deep seated candidiasis [ 2 , 3 ] and there has been rise in the incidence of antifungal resistance over the past decade [ 4 , 5 ], it is important to evaluate the development of resistance pattern. Method of typing the organism based on the total protein expressed is useful in characterizing and understanding the development of drug resistance.…”

Section: Introductionmentioning

confidence: 99%

Total Protein Profile and Drug Resistance inCandida albicansIsolated from Clinical Samples

Zaidi

Mani

Thawani

et al. 2016

Molecular Biology International

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This study was done to assess the antifungal susceptibility of clinical isolates of Candida albicans and to evaluate its total protein profile based on morphological difference on drug resistance. Hundred and twenty clinical isolates of C. albicans from various clinical specimens were tested for susceptibility against four antifungal agents, namely, fluconazole, itraconazole, amphotericin B, and ketoconazole. A significant increase of drug resistance in clinical isolates of C. albicans was observed. The study showed 50% fluconazole and itraconazole resistance at 32 μg mL−1 with a MIC50 and MIC90 values at 34 and 47 and 36 and 49 μg mL−1, respectively. All isolates were sensitive to amphotericin B and ketoconazole. The SDS-PAGE protein profile showed a prevalent band of ~52.5 kDa, indicating overexpression of gene in 72% strains with fluconazole resistance. Since the opportunistic infections of Candida spp. are increasing along with drug resistance, the total protein profile will help in understanding the evolutionary changes in drug resistance and also to characterize them.

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Prevalence and Antifungal Susceptibility of Candida Species Isolated From Patients Attending Tertiary Care Hospital

Abstract: Abstract

Cited by 11 publications

References 9 publications

Antifungal Susceptibility and Biofilm Production of Candida spp. Isolated from Clinical Samples

Antifungal Susceptibility and Biofilm Production of Candida spp. Isolated from Clinical Samples

Speciation and antifungal susceptibility testing of Candida isolates in various clinical samples in a tertiary care hospital in Mumbai

Total Protein Profile and Drug Resistance inCandida albicansIsolated from Clinical Samples

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