Total Protein Profile and Drug Resistance inCandida albicansIsolated from Clinical Samples

Abstract

This study was done to assess the antifungal susceptibility of clinical isolates of Candida albicans and to evaluate its total protein profile based on morphological difference on drug resistance. Hundred and twenty clinical isolates of C. albicans from various clinical specimens were tested for susceptibility against four antifungal agents, namely, fluconazole, itraconazole, amphotericin B, and ketoconazole. A significant increase of drug resistance in clinical isolates of C. albicans was observed. The study showed 50% fluconazole and itraconazole resistance at 32 μg mL−1 with a MIC50 and MIC90 values at 34 and 47 and 36 and 49 μg mL−1, respectively. All isolates were sensitive to amphotericin B and ketoconazole. The SDS-PAGE protein profile showed a prevalent band of ~52.5 kDa, indicating overexpression of gene in 72% strains with fluconazole resistance. Since the opportunistic infections of Candida spp. are increasing along with drug resistance, the total protein profile will help in understanding the evolutionary changes in drug resistance and also to characterize them.

Total protein level reduction of odontopathogens biofilms by Rosmarinus officinalis L. (rosemary) extract: an analysis on Candida albicans and Streptococcus mutans

“…Total protein concentration of antifungalresistant C. albicans samples has varied from 1.05 to 3.22 mg/mL after exposure to the different therapeutics agents, such as fluconazole, itraconazole, amphotericin B, and ketoconazole. These levels have been lower than those presented by untreated samples [36]. Similarly, in our study the total protein level of the C. albicans and S. mutans biofilms was decreased by the treatment with R. officinalis L. extract (Figures 12).…”

Antifungal drugs resistance profiles of C. albicans strains isolated from the oral cavity of children and adolescents

“…fluconazole, natamycin, and ketoconazole (11). Similarly, in an Indian study, where a hundred and twenty clinical isolates of C. albicans from various clinical specimens were tested for susceptibility against four antifungal agents: fluconazole, itraconazole, amphotericin B, and ketoconazole, a significant increase of drug resistance in clinical isolates of C. albicans was observed (12). Araj (13).…”

Extract of Syzygium aromaticum suppress eEF1A protein expression and fungal growth

“…When the drug is not intended to kill or inhibit a pathogen, then the term is equivalent to dosage failure or drug tolerance (Zaidi et al . ). Antibiotic resistance is a natural phenomenon resulting from the modern selective pressure of the clinical use of antibiotics (D'Costa et al .…”

“…The intensive use of antibiotics made these fungi more drug resistant and reduced the effectiveness of drugs in curing a disease or condition. When the drug is not intended to kill or inhibit a pathogen, then the term is equivalent to dosage failure or drug tolerance (Zaidi et al 2016). Antibiotic resistance is a natural phenomenon resulting from the modern selective pressure of the clinical use of antibiotics (D'Costa et al 2011).…”