Extract of <i>Syzygium aromaticum</i>
 suppress eEF1A protein expression and fungal growth

Wang, Y.; Ding, Yiran; Wang, S.; Chen, H.; Zhang, H.; Chen, Wei; Gu, Zhaoliang; Chen, Y.Q.

doi:10.1111/jam.13478

Cited by 8 publications

(5 citation statements)

References 49 publications

(88 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results revealed that the crude extract contained 18 different chemical compounds with different concentrations such like eugenol, trans-caryophyllene, humulene, anthracenedione, cedran-diol, citroflex A and lucenin 2. This result is consistent with that of Wagn et al (2017) who found that the major compounds in clove alcoholic extract analysis by GC/MS were eugenol, acetyl eugenol , caryophyllene , and humulene followed by a-farnesene and caryophylleneoxide. These notable differences in composition may be due to extraction methods as well as genetic diversity and agronomic treatments (Mahanta et al, 2007).…”

Section: Discussionsupporting

confidence: 93%

In vitro evaluation of Syzygium aromaticum L. ethanol extract as biocontrol agent against postharvest tomato and potato diseases

Soliman¹,

Bous²,

Ibrahim³

et al. 2018

Egypt. J. Bot.

View full text Add to dashboard Cite

E thaNolic extract of dried flower bud of Syzygium aromaticum l. (clove) was investigated to evaluate its antifungal activity against local fungal isolates causing postharvest infections form potato and tomato. Four fungal isolates; Geotrichum candidum, Alternaria alternata, Fusarium oxysporum and Mucor hiemalis were identified. All fungal isolates were found to be inhibited by the extract; whereas the smallest inhibition zone) of S. aromaticum extract was 2.5% for M. hiemalis, A. alternata, G. candidum and 12.5% for F. oxysporum. Quantitative assessment of phytochemical compounds in clove plant revealed the presence of phenolic acids (20.80%); flavonoids (26.81%); tannins (4.90%), saponins (2.60%), alkaloids (1.60%), total protein (17.83%), total carbohydrate (2.23%) and total oil (0.90%). Biochemical profile was revealed by GC/MS which detect 18 different chemical compounds. The major compounds were eugenol (17.27%), trans-caryophyllene (0.50%), humulene (3.33%), anthracenedione (3.35%), cedran-diol (0.61%), citroflex A (5.45%) and lucenin 2 (8.25%). TLC technique and different solvent systems were employed to select the proper one based on its capacity to isolate the maximum number of fluorescent spots in crude extract. Chloroform-acetone-butanol (CAB) 85:15:20 could efficiently separate three bands (A, B and C). Each detected band was individually screened for its antifungal activity against the isolated fungi. Only band C showed a significant antifungal activity. Mass spectroscopy (MS) was used to investigate characteristics, purity as well as expectation of both chemical formula and molecular weight of the isolated compounds; which finally proved that glucoside β-sitosterol was the main compound represented in fraction-C.

show abstract

Section: Discussionsupporting

confidence: 93%

In vitro evaluation of Syzygium aromaticum L. ethanol extract as biocontrol agent against postharvest tomato and potato diseases

Soliman¹,

Bous²,

Ibrahim³

et al. 2018

Egypt. J. Bot.

View full text Add to dashboard Cite

show abstract

“…In addition, we found the expression of phosphopyruvate hydratase activity-related protein CB033636 (spot 31) was increased in SP4, CP2 and CP4 samples, but decreased in the SP2 sample. The phosphopyruvate hydratase was reported to be involved in the defense against fungi [ 70 , 71 ]. Previous studies have shown that a molecule associated with powdery mildew resistance, NORTIA (NTA), plays a role in the process of reception pollen tubes in synergids, suggesting that this protein is involved in both pollen tube acceptance and powdery mildew infections [ 72 ] and the decrease in SP2 may contribute to pollination response at early stage.…”

Section: Resultsmentioning

confidence: 99%

Comparative Proteomics Analyses of Pollination Response in Endangered Orchid Species Dendrobium Chrysanthum

Wang

et al. 2017

IJMS

View full text Add to dashboard Cite

Pollination is a crucial stage in plant reproductive process. The self-compatibility (SC) and self-incompatibility (SI) mechanisms determined the plant genetic diversity and species survival. D. chrysanthum is a highly valued ornamental and traditional herbal orchid in Asia but has been declared endangered. The sexual reproduction in D. chrysanthum relies on the compatibility of pollination. To provide a better understanding of the mechanism of pollination, the differentially expressed proteins (DEP) between the self-pollination (SP) and cross-pollination (CP) pistil of D. chrysanthum were investigated using proteomic approaches—two-dimensional electrophoresis (2-DE) coupled with tandem mass spectrometry technique. A total of 54 DEP spots were identified in the two-dimensional electrophoresis (2-DE) maps between the SP and CP. Gene ontology analysis revealed an array of proteins belonging to following different functional categories: metabolic process (8.94%), response to stimulus (5.69%), biosynthetic process (4.07%), protein folding (3.25%) and transport (3.25%). Identification of these DEPs at the early response stage of pollination will hopefully provide new insights in the mechanism of pollination response and help for the conservation of the orchid species.

show abstract

“…CEO interferes with the expression of virulence-related genes involved in the flagellum, PEB1, PEB4, LPS and serine protease, with significant antibacterial and potentially virulence-modulating effects on Campylobacter jejuni (81). eEF1A protein interacted with several viral proteins, leading to enhanced viral replication, reduced apoptosis and increased cellular transformation; therefore, Wang et al (82) suggested that downregulation of eEF1A protein expression may be another important mechanism for exerting its antifungal activity.…”

Section: Antibacterial Activitymentioning

confidence: 99%

Recent advances in nutritional composition, phytochemistry, bioactive, and potential applications of Syzygium aromaticum L. (Myrtaceae)

Xue¹,

Xiang²,

Wang³

et al. 2022

Front. Nutr.

View full text Add to dashboard Cite

Syzygium aromaticum is an aromatic plant native to Indonesia, and introduced to tropical regions worldwide. As an ingredient in perfumes, lotions, and food preservation, it is widely used in the food and cosmetic industries. Also, it is used to treat toothache, ulcers, type 2 diabetes, etc. A variety of nutrients such as amino acids, proteins, fatty acids, and vitamins are found in S. aromaticum. In addition to eugenol, isoeugenol, eugenol acetate, β-caryophyllene and α-humulene are the main chemical constituents. The chemical constituents of S. aromaticum exhibit a wide range of bioactivities, such as antioxidant, antitumor, hypoglycemic, immunomodulatory, analgesic, neuroprotective, anti-obesity, antiulcer, etc. This review aims to comprehend the information on its taxonomy and botany, nutritional composition, chemical composition, bioactivities and their mechanisms, toxicity, and potential applications. This review will be a comprehensive scientific resource for those interested in pursuing further research to explore its value in food.

show abstract

Extract of Syzygium aromaticum suppress eEF1A protein expression and fungal growth

Cited by 8 publications

References 49 publications

In vitro evaluation of Syzygium aromaticum L. ethanol extract as biocontrol agent against postharvest tomato and potato diseases

In vitro evaluation of Syzygium aromaticum L. ethanol extract as biocontrol agent against postharvest tomato and potato diseases

Comparative Proteomics Analyses of Pollination Response in Endangered Orchid Species Dendrobium Chrysanthum

Recent advances in nutritional composition, phytochemistry, bioactive, and potential applications of Syzygium aromaticum L. (Myrtaceae)

Contact Info

Product

Resources

About