Background: Cervical cancer caused by human papilloma virus (HPV), is the second most common cancer for women. This cancer is distributed worldwide, with ~80% of cases are found in the developing countries. In Indonesia, data of HPV genotypes are still limited and do not represent all regions of the country. Thus, here we report genotyping of HPV samples collected from the Dr. Soetomo Hospital Surabaya Indonesia patients, in 2013. Materials and Method: A cross sectional study was performed using 68 paraffin blocks of low-grade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL), and squamous cell carcinoma (SCC) cervix.

ABSTRAK Objectives: To report one malignant Brenner tumor (MBT) case in Dr. Soetomo Hospital, Surabaya, Indonesia, that underwent therapy from August 2016 to August 2017. Case report: A woman aged 62 years underwent total abdominal hysterectomy and bilateral salphingo-oophorectomy surgery with histopathologic results of malignant Brenner Tumor. From immunohistochemical examination the expression of p63 was positive. The patient had been treated with Paclitaxel carboplatin chemotherapy for 5-cycles, with the results of the last postchemotherapy evaluation showing no recidive mass. Conclusion: There was only one case of malignant ovarian Brenner Tumor in Dr Soetomo Hospital, Surabaya, Indonesia, in 2014-2016. The diagnosis can only be based on anatomic pathology examination. The specific tumor markers for malignant Brenner tumors have so far not been found. Keywords: Malignant Brenner Tumor; epithelial ovarian tumor; p63. Tujuan: Melaporkan satu kasus pasien dengan tumor Brenner di RSUD Dr. Soetomo, Surabaya, Indonesia, yang menjalani terapi dari bulan Agustus 2016 hingga Agustus 2017. Laporan kasus: Seorang wanita berusia 62 tahun menjalani histerektomi abdominal total dan operasi salphingo-ooforektomi bilateral dengan hasil histopatologis Tumor Brenner ganas. Dari pemeriksaan imunohistokimia, ekspresi p63 didapatkan positif. Pasien telah dirawat dengan kemoterapi Paclitaxel carboplatin selama 5 siklus, dengan hasil evaluasi post-kemoterapi terakhir tidak menunjukkan massa residif. Simpulan: Hanya terdapat satu kasus Tumor Brenner ovarium ganas di RSUD Dr Soetomo, Surabaya, Indonesia, antara 2014-2016. Diagnosis hanya dapat didasarkan pada pemeriksaan patologi anatomi. Penanda tumor spesifik untuk tumor Brenner ganas sejauh ini belum ditemukan. Kata kunci: Tumor Brenner ganas; tumor epitel ovarium; p63

Approximately 20-30% of all cervical cancer cases are adenocarcinoma and adenosquamous carcinoma. Around 70% of all of these types of cancer are related to infection of Human Papillomavirus (HPV). This study evaluated the distribution of HPV genotype in cervical adenocarcinoma and adenosquamous carcinoma. A cross-sectional study was conducted at the Department of Anatomic Pathology, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia, from January to December 2015. The sample were 22 formalin-fixed paraffin-embedded (FFPE) of cervical adenocarcinoma tissues and adenosquamous carcinoma tissues. FFPE was used for DNA extraction and followed with HPV genotyping to detect 40 genotypes of HPV, including low risk (LR) and high risk (HR) HPV. The histopathological types of adenocarcinomas were adenocarcinoma NOS and mucinous adenocarcinoma, while the adenosquamous carcinoma types were adenosquamous carcinoma and adenosquamous carcinoma glassy. All of the specimens were infected by HPV. In cervical adenocarcinoma, the infection was by HPV 6, 11, 16, 18, 31, 45, 68B, and 72, and in adenosquamous carcinoma by HPV 6, 16, 18, 45, and 59. HPV 18 was predominant, which was found in 13/22 (59.1%) in adenocarcinoma and 19/22 (86.4%) in adenosquamous carcinoma. Single infection and multiple infections in adenocarcinoma were 13/22 (59.1%) and 9/22 (40.9%), while in adenosquamous carcinoma were 21/22 (95.5%) and 1/22 (4.5%) respectively. The most common HR HPVs found in this study were HPV 18, HPV 45, HPV 16 and LR HPV are HPV 11, HPV 6.

Colorectal adenocarcinoma is positioned as the third of most common cancer which the cases rise in Indonesia lately. More than 90% of colorectal carcinoma are adenocarcinoma type. One of prognostic factor of colorectal adenocarcinoma is invasion state of the tumor (T). Uncontrollable proliferation of tumor cell causes transformation of paracellular permeability that increase claudin-4 expression (a protein located on main integral membrane). Claudin-4 activation influence the expression and activity of MMP-2 directly or by altering transduction signal. Expression of claudin-4 and MMP-2 play a role in tumor invasion. Analyzing correlation of claudin-4 and MMP-2 toward invasion state of the tumor (T stadium) on colorectal adenocarcinoma. Analytical observation was conducted on 47 samples of colorectal adenocarcinoma with different invasion state collected by Laboratory of Pathological Anatomy, Dr. Soetomo General Academic Hospital during 2015-2018. Immunohistochemistry was conducted using both claudin-4 and MMP-2 antibodies. Expression of claudin-4 and MMP-2 were semiquantitatively measured then statistically analyzed. Significant result could be obtained in comparison between claudin-4 and tumor invasion state (p=0.773). The significant result could be obtained in comparison between MMP-2 and tumor invasion state (p=0.920). It also could be observed in comparison between claudin-4 and MMP-2 (p=0.638). In summary, claudin-4 and MMP-2 play a role on tumor invasion colorectal adenocarcinoma. It was showed by significant result between claudin-4 and MMP-2 expressions compared to invasion state of colorectal adenocarcinoma.

Tujuan: Penelitian ini bertujuan untuk mengetahui prevalensi lesi prekanker serviks dengan cara melakukan skrining kanker serviks menggunakan pemeriksaan Pap smear.Bahan dan Metode: Penelitian ini merupakan penelitian observasional deskriptif dengan metode pendekatan cross sectional yang dilakukan pada 140 wanita, terdiri dari 90 orang dari Puskes-mas Tanah Kali Kedinding Surabaya, dan 50 orang dari Rumah Sakit Mawadah Mojokerto, dengan usia 20-70 tahun. Pemeriksaan spesimen sitologi serviks dengan pengecatan Papaniculaou dan klasifikasi hasil pemeriksaaan sitologi berdasar-kan klasifikasi Papaniculaou dan sistim Bethesda. Pemeriksaan inspeksi visual asam asetat (IVA) adalah metode lain untuk skrining kanker serviks dilakukan dengan mengoleskan asam asetat 5% pada area serviks dan melakukan pengamatan satu menit kemudian.Hasil: Hasil pemeriksaaan Pap smear menunjukkan Papaniculaou kelas I (sama dengan normal pada klasifikasi sistim Bethesda) yaitu 12.1%, kelas II (sama dengan NILM pada klasifikasi sistim Bethesda) yaitu 86, 4%, dan kelas III (sama dengan LSIL pada klasifikasi sistim Bethesda) yaitu 1,4%. Hasil pemeriksaan IVA menunjukkan 6,43% positif dan 93,57% negatif. Hasil pemeriksa-an IVA positif terdapat pada 9/140 orang yang merupakan proses keradangan dan infeksi, bukan merupakan lesi prekanker.Simpulan: Prevalensi lesi prekanker yaitu 1,4%. Pemeriksaan IVA menunjukkan hasil positif semu yang disebabkan oleh proses radang atau infeksi pada serviks.

Cholestatic infants are associated with congenital abnormalities or viral infections, such as cytomegalovirus (CMV) infection. CMV can be detected by polymerase chain reaction (PCR) in body fluids, including urine which can be obtained easily and is non-invasive. The objective was to detect CMV in urine specimens of cholestasis infants and to analyze its correlation with serological status. This was a descriptive observational study with the cross-sectional approach, used urine from 39 cholestatic infants who meet the inclusion and exclusion criteria and have been approved by Ethics Committee. The nested-PCR was performed from extracted urine and unextracted direct urine. Serological data of immunoglobulin (Ig) M and IgG data were collected. Data were analyzed by Chi-square. Detection of CMV from extracted urine by PCR showed positive in 87.2% patients and from unextracted urine was positive in 48.7% patients. Serological status showed that IgM was positive in 41.0% patients and IgG was positive in 89.7% patients. The acute infection (IgM+ IgG+) was found in 41.0% patients, past infection (IgM-IgG+) was 48.7% patients, and not infected (IgM-IgG-) was in 10.3% patients. The acute infection (IgM+ IgG+), past infection (IgM-IgG+) and not infected (IgM-IgG-) was found in 41.0%, 48.7%, and 10.3% patients, respectively. The correlation between PCR CMV from extracted urine with serological CMV was moderate, while the unextracted urine was low. It indicates that to detect the infection of CMV, PCR technique is more accurate than serological testing, and the extracted urine is more appropriate specimen as PCR template than direct urine.

Introduction: Breast cancer and cervical cancer are common cancers in females. Both of these cancers can be detected early. Early detect vion can reduce cancer morbidity and mortality. The community outreach activities are required to increase knowledge about breast cancer and cervical cancer, in addition to screening as preventive efforts for these two cancers.Methods: The activity of raising knowledge about the prevention and early detection of breast cancer and cervical cancer was carried out through community counseling to 94 females who were members of the PKK and young females in the Kecamatan Proppo, Kabupaten Pamekasan. Pre and post tests were given before and after the community counseling. After the community counseling, 86 participants were screened for breast cancer by clinical palpation and 46 participants were screened for cervical cancer using the pap smear method.Results: The result of the post test showed a 27.53% increase in knowledge. The results of the pap smear examination on participants showed 96% concluded as Papanicolau Class II, 2% Class I, and 2% Class IV. The patient with class IV results was referred to an obstetrician.Conclusion: Community counseling about breast self examination (BSE) and Pap smear can increase people's knowledge about early detection of breast cancer and cervical cancer.

Human papillomavirus (HPV) infection is one of the most common sexually transmitted infections. HPV high risk (HPVHR) were HPV16,18 related with invasive penile carcinomas, and HPV low risk (HPVLR) were HPV6,11 related to anogenital warts. Male infection is usually asymptomatic that it would be explain increasing the incidence of HPV associated cancers. Identification HPV genotype is very important for predicting the development of the diseases, to be benign or malignant cancer. The objective of this study was to identify the genotype of HPV that infect men with anogential warts. This research used 12 biopsy specimens from men patient with anogenital warts at Outpatient clinic of Department Dermatology and Venereology, Dr. Soetomo General Hospital period 2016-2017. The specimens were diagnozed by pathologist and HPV gentoyping was done to detect 40 HPV genotype including HPVHR and HPVLR. The result showed that 58% (7/12) were positive for HPVLR and 42% (5/12) were positive for HPV LR/HR. The genotype HPV that infected men patient with anogenital warts is HPVLR (HPV6,11) and HPVHR (HPV18,51,52,82) with single infection of HPVLR or mutiple infection HPVLR/LR or HPVLR/HR. The infection of HPVHR would be develops to be malignant transformation. It suggested that HPV genotype needs to be checked the for the anogenital warts cases for predicting the development of the diseases.