The Genotype of Human Papilloma Virus of Male Patient with Anogenital Warts

Background: Warts are common viral infections of the skin that are caused by human papillomavirus (HPV). There are more than 200 HPV genotypes up till now. Objective:To identify HPV genotype distribution among wart patients. Methodology: A cross sectional study was conducted on 78 patients with warts. Shave biopsy was taken from wart cases for detection of HPV by conventional PCR. HPV genotyping was performed using real time PCR. Results: HPV DNA was detected in 50 (64.1%) of all biopsy samples while 28 (35.9%) of samples were negative. The most frequently detected genotype was HPV-52 (7.1 %) from HPV positive samples. Conclusion: obtaining results can increase the knowledge regarding the prevalence of HPV and its type distribution in Egypt. This may contribute to create a national prevention program for HPV.

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“…Murtiastutik et al, 23 found that HPV 6, 11 were the predominant low risk genotypes in a study conducted on 12 males with anogenital warts.…”

Section: Discussionmentioning

confidence: 96%

Genotyping of Human papilloma virus from warts in Egyptian patients

Tantawy

El-Beyali²,

Gohar³

et al. 2020

Egyptian Journal of Medical Microbiology

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“…Recently, there are 3 commercial vaccines against HPV infection, namely Gardasil to prevent infections caused by HPV 6, 11, 16, and 18, Cervarix to prevent infections caused by HPV 16, and 18, and Gardasil 9 to prevent infections caused by HPV 6, 11, 61, 18, 31, 33, 45, 52, and 58 (Gupta et al, 2017). A previous study on anogenital benign lesions showed that HPV genotype in CA patients was dominated by HPV 11 and HPV 6 among 13 female patients and 12 male patients (Murtiastutik et al, 2019). In pre-cancerous lesion and cancerous lesion of uterine cervix, those were dominated by HPV 16 (62.68%), then followed by HPV 18 (20.9%), HPV 45 (5.97%), HPV 52 (5.97%), and HPV 67 (4.48%) (Mastutik et al, 2018).…”

Section: P16 Ink4a Expression In Ca Lesionsmentioning

confidence: 91%

p16INK4A Expression in Condyloma Acuminata Lesions Associated with High-Risk Human Papillomavirus Infection

Mastutik

Rahniayu

Arista

et al. 2021

Asian Pac J Cancer Prev

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Objective: The objective of this study was to discover the possible correlation between p16INK4A expression and the LR/HR-HPV infection in condyloma acuminate (CA) lesions. Materials and Method: This cross-sectional study was conducted during January-December 2017 on 33 CA patients. The expression of p16 INK4A was detected by immunohistochemistry (IHC) staining. The positive interpretation was carried out by scoring which score 0 was negative, score 1 was sporadic, score 2 was focal, and score 3 was diffuses. The HPV genotypes were identified by reverse line blot, and 40 genotypes of HPV detected, including HR-HPV (HPVs 16, 18, 26, 31, 33,35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 67, 68a, 68b, 69, 73, and 82) and LR-HPV (HPVs 6, 11, 40, 42, 43, 44, 54, 55, 61, 62, 64, 70, 71, 72, 81, 83, 84, 87, 89, and 90). Results: The expression of p16 INK4A was significantly correlated with HR-HPV infection. Patients infected with HR-HPV had 0.644 times higher possibility to express p16 INK4A gene compared to those infected with LR-HPV. LR-HPV genotypes detected in CA patients were HPVs 6, 11, 42, 61, 54, 81, 87, 89, and 90 and HR-HPV genotypes were HPVs 18, 26, 45, 51, 52, 67, 68B, 69, and 82. LR-HPV was found in 19/33 of patients and HR-HPV was in 14/33 of patients. The expression of p16 INK4A in CA lesions was diffuse in15.2% of patients, was focal in 24.2% of patients , was sporadic in 39.4% of patients were, and was negative in 21.2% of patients . In LR-HPV group, there was no diffuse expression, focal expression was observed in 15.8%, sporadic in 47.4%, and negative in 36.8%, while in HR-HPV group, p16 INK4A expression was detected in all lesions , in a way that its expression was diffuse in 35.7%, focal in 35.7%, and sporadic in 28.6%. Conclusion: IHC is a routine method in histopathological diagnosis, therefore the detection of p16 INK4A expression by IHC can be used as a biomarker for HR-HPV infection diagnosis.

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Distribution Genotype High Risk (HR) And Low Risk (LR) Human Papillomavirus (HPV) at Condyloma Acuminata

Mastutik

Rahniayu

Murtiastutik

et al. 2021

BHSJ

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Introduction: Condyloma acuminata that is also known as genital warts are one of the most common sexually transmitted that caused by infection of Human papillomavirus (HPV). Persistent infection of Low Risk (LR) or High risk (HR) HPV is a risk factor for progress into benign or malignant cancer. The objective is to analyze distribution of genotype LR-HPV and HR-HPV at condyloma acuminata in anogenital region. Methods: A cross sectional study using were 36 lesions from men and women of condyloma acuminata patients. All subject signed the informed consent and ethic obtained from our institution, number 382/Panke.KKE/V/2016. The specimen was used to histopathological examination and to identified 40 genotypes of HPV using a reverse line blot assay.Results: The All patients were diagnosed as condyloma acuminata, some with focus dysplasia and koilocytosis. All patients were positive for HPV, including LR-HPV were HPV 6, 11, 42, 54, 61, 81,87,89 and HR-HPV were HPV 18, 26, 45, 51, 52, 66, 67, 68B, 69, 82. The single infection of LR-HPV was 44.4%, multiple infection LR/LR-HPV was 13,9% and the multiple infection of LR/HR-HPV was 41.7%. The LR-HPV infected 70,6% and HR-HPV infected 29,4%. Conclusion: LR-HPV is the major infection of condyloma acuminata, in single infection or multiple infection with HR-HPV. The most common infections were HPV 11, followed by HPV 6, HPV 18, HPV 51, and HPV 82. The determination of genotype of HPV can be used to predict the malignant transformation.

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The Genotype of Human Papilloma Virus of Male Patient with Anogenital Warts

Cited by 3 publications

References 10 publications

Genotyping of Human papilloma virus from warts in Egyptian patients

Genotyping of Human papilloma virus from warts in Egyptian patients

p16INK4A Expression in Condyloma Acuminata Lesions Associated with High-Risk Human Papillomavirus Infection

Distribution Genotype High Risk (HR) And Low Risk (LR) Human Papillomavirus (HPV) at Condyloma Acuminata

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