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The aim of study was to compare the effects of supplementation of food with herbal oils (Thymus vulgaris and Foeniculum vulgare) on biochemical properties and electrolytes of rainbow trout infected with Yersinia ruckeri. In total, 120 healthy fish (mean weight 84 ± 1.02 g) were equally divided into four experimental groups. The experimental study was carried out for one week. The first group was control without supplementation and infection, the second group was infected and without oil supplementation, the third group was supplemented with oils (10 ml·100 g -1 rates) for one week and infected with Y. ruckeri and the last group was oil supplemented without infection. Results indicated that fish fed with dietary supplements showed enhanced bactericidal activity, total protein, albumin, cholesterol, triglyceride and bilirubin compared to the control (P < 0.05). As the value of herbal oils was increased in diets, the plasma glucose level decreased. The levels of K, Na, Ca, and Mg increased whereas Cl values decreased, compared to the control. It can be concluded that diet supplementation with herb oils used in this study can increase disease resistance by increasing levels of some biochemical parameters and electrolytes in rainbow trout to Y. ruckeri infection.

Objective / Purpose: Bacteria causes one of the most common types of chronic mastitis. The most common causative organisms of mastitis include: Staphylococci, Streptococci and coliforms. The scope of this work was to research the antibacterial effects of Piper nigrum extracts against mastitis pathogens, and its antioxidant capacity. Materials and Methods: In our study, 2 Staphylococcus aureus and 5 Coagulase Negative Staphylococcus were used for experiments. Additionally, Piper nigrum were collected from Mugla herbalists in Turkey. The plant extracts were tested by disc diffusion assay for antibacterial activity. The antioxidant activities of plant extracts were also determined by ABTS [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] method. Results: The methanol extract of plant showed maximum inhibition zone against 2 bacteria. MIC values of extracts are 6500 μg/mL. The value found in ABTS method is highly effective (75.7%). Discussion/Conclusion: As a result, the in vitro studies indicate that the extracts of Piper nigrum have significant antibacterial and antioxidant activities. In addition to, the plant extracts could be used in treating mastitis caused by the test bacteria.

Use of a simpler, faster and reliable method for identification of species of origin in fresh and processed meat products is required to prevent unethical practices that may occur in the meat industry. The effectiveness of a random amplified polymorphic DNA (RAPD) method for identification of fresh meats from cattle, goat, sheep, camel, pork, wild swine, donkey, cat, dog, rabbit or bear origin was evaluated using a 10‐base primer (ACGACCCACG). The method was also used to determine the species in a 1 : 1 mix of raw minced meat from sheep‐pork, horse‐beef or beef‐sheep. Characteristic RAPD profiles for each species were obtained. However, efficacy of the technique in identifying species in meat mixtures varied depending on the species in the mix. These results indicate that RAPD may be useful for identification of meat samples from single species, such as intact meat samples, whereas caution should be exercised in identification of origin of species in minced meat that may consist of multiple species.

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Prostate cancer is the most commonly diagnosed malignancy and the second most common cause of cancer deaths in the Western male population. Matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) modulate the remodeling of the extracellular matrix (ECM). The imbalance between MMPs and TIMPs may lead to an emergence of pathological processes such as cancer. In this study, the association between TIMP-2 (-418 G/C) and MMP-2 (-1306 C/T) polymorphisms and prostate cancer in the Turkish population was investigated.Materials and methods: Sixty-one prostate cancer patients and 46 healthy subjects were included in the study. DNA was isolated from 2 mL of peripheral blood taken from subjects, and genotypes were analyzed by the polymerase chain reaction-restriction fragment length polymorphism method. Results:The TIMP-2 -418 (GC) genotype was found in 15 cases (32.6%) in the control group and in 9 cases (14.8%) in the patients group, and statistical significance was determined (P = 0.037, OR = 0.346). The MMP-2 -1306 (CT) genotype was found 2.17 times more in the patient group than in the control group (P = 0.149, OR = 2.17). Conclusion:Our results show that the TIMP-2 -418 (GC) genotype had a putative protective effect against prostate cancer.

Objective: Microorganisms have an easier entry into the sportsman's epidermis, and these make diseases on athletes. The main scope of the study is to search the lack of information about the biological activities of Mentha piperita extracts against bacteria isolated from soccer player's shoes. Materials and Methods: The bacteria were isolated from soccer player's shoes from Balikesir Spor soccer team after the competition. The plant extracts were tested by disc diffusion assay for antibacterial activity. In addition, the different extracts of plant were screened by ABTS decolorization assay for antioxidant activity. Results: The highest inhibition zone in bacteria were determined on Staphylococcus sp. BFT12 (21 mm). MIC value was determined as 3250 µg/mL. The highest antioxidant activity of the plant was determined from aqueous extract of plant. This scavenging activity is about 88%. Discussion/Conclusion: The extracts of the plant have high antibacterial and antioxidant potential.

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