The aim of study was to compare the effects of supplementation of food with herbal oils (Thymus vulgaris and Foeniculum vulgare) on biochemical properties and electrolytes of rainbow trout infected with Yersinia ruckeri. In total, 120 healthy fish (mean weight 84 ± 1.02 g) were equally divided into four experimental groups. The experimental study was carried out for one week. The first group was control without supplementation and infection, the second group was infected and without oil supplementation, the third group was supplemented with oils (10 ml·100 g -1 rates) for one week and infected with Y. ruckeri and the last group was oil supplemented without infection. Results indicated that fish fed with dietary supplements showed enhanced bactericidal activity, total protein, albumin, cholesterol, triglyceride and bilirubin compared to the control (P < 0.05). As the value of herbal oils was increased in diets, the plasma glucose level decreased. The levels of K, Na, Ca, and Mg increased whereas Cl values decreased, compared to the control. It can be concluded that diet supplementation with herb oils used in this study can increase disease resistance by increasing levels of some biochemical parameters and electrolytes in rainbow trout to Y. ruckeri infection.

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This study is aimed to determine the bioaccumulation of copper and zinc in hemolymph of freshwater crayfish (Astacus leptodactylus) fed with zebra mussel (Dreissena polymorpha) collected from Keban Dam Lake. This is the first study on bioaccumulation of Cu and Zn in hemolymph of males and females of A. leptodactylus. The average concentrations of Cu and Zn in the hemolymph samples were found out as 0.54 and 35.07 ppm respectively for male and 0.42 and 15.59 ppm respectively for female control freshwater crayfish. The levels of Zn in the hemolymph of both male and female ones among control and diet groups were statistically significant (P < 0.05). The Cu levels among control and diet groups in female ones were statistically significant (P < 0.05) but not in the male ones (P > 0.05). The results of current study revealed that feed treatment with boiling was not effective at the levels of metal bioaccumulation in hemolymph. It was observed that this metal bioaccumulation is more related with sex differences.