Effect of method of cooking on identification of heat processed beef using polymerase chain reaction (PCR) technique

Arslan, Ali; İlhak, Osman İrfan; Çalıcıoğlu, Mehmet

doi:10.1016/j.meatsci.2005.08.001

Cited by 128 publications

(95 citation statements)

References 30 publications

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“…Every animal has a unique combination of restriction fragments (Minarovic et al, 2010). Species determination by PCR was affected by cooking temperature, time, and size of the DNA fragment to be amplified (Martinez & Yman, 1998;Matsunaga et al, 1999;Arslan et al, 2006). This research showed only six bands in tube 1 (i.e.…”

Section: Specific Fragments Amplification Of Cyt β Gene On Dog Cat mentioning

confidence: 86%

Species Authentication of Dog, Cat, and Tiger Using Cytochrome β Gene

Irine¹,

Nuraini²,

Sumantri³

2013

Med Pet

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Adulteration of animal food products for economic reason has happened during the last decades. Species identification method development was needed to prevent falsification information. The objective of this research was to study species authentication (dog, cat, and tiger) to ensure animal origin in products using cyt β gene specific marker. DNA extraction and fragment amplification were conducted using phenol-chloroform and multiplex PCR (Polymerase Chain Reaction) method, respectively. This research showed that fragment length of amplification for species tested (dog, cat, and tiger) were 523, 331, 319 bp, respectively. Species specificity was also indicated by high reverse primers homology percentage. Multiplex PCR technique succeed to amplify DNA fragment from species tested, but has a limitation to amplify total DNA composite of mix DNA. Key words: cat, cytochrome β gene, dog, multiplex PCR, tiger ABSTRAKUpaya pemalsuan produk pangan asal ternak dengan alasan ekonomi masih sering terjadi hingga saat ini. Pengembangan metode identifikasi spesies diharapkan dapat melindungi konsumen dari pemalsuan informasi. Penelitian ini bertujuan untuk mempelajari pembuktian spesies anjing, kucing, dan harimau menggunakan marka spesifik berbasis gen sitokrom β (cyt β). Ekstraksi DNA dilakukan dengan metode fenol-kloroform, semetara amplifikasi fragmen DNA menggunakan metode multipleks PCR. Penelitian ini menunjukkan bahwa amplifikasi panjang fragmen pada spesies anjing, kucing, dan harimau adalah 523, 568, dan 319 pb. Selain itu, kespesifikan spesies juga ditunjukkan dengan persentase homologi primer reverse yang tinggi pada masing-masing spesies. Metode multipleks PCR berhasil mengamplifikasi fragmen DNA dari semua spesies yang diuji, namun mempunyai keterbatasan dalam mengamplifikasi gabungan DNA total semua spesies.

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Section: Specific Fragments Amplification Of Cyt β Gene On Dog Cat mentioning

confidence: 86%

Species Authentication of Dog, Cat, and Tiger Using Cytochrome β Gene

Irine¹,

Nuraini²,

Sumantri³

2013

Med Pet

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“…The disadvantages of protein-based methods can be solved with methods based on the presence of species-specific DNA sequences, detected by techniques such as PCR. The amplification of specific DNA sequences by PCR provides a rapid, sensitive, and specific method for detection of animal tissues in food and feed (1).…”

Section: Discussionmentioning

confidence: 99%

DNA based approaches for the detection and identification of bovine meat and bone meal in feeds

Weiner

Golebiowska

Paprocka

et al. 2013

Bulletin of the Veterinary Institute in Pulawy

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The aim of this paper was to present the results of comparative evaluation of the usefulness of PCR method for the detection and identification of bovine DNA in feeds. In the validation study, the limit of detection for PCR was determined as 0.05% for bovine meat and bone meal (MBM). Among 132 feed samples, bovine DNA was detected in eight (6.06%) samples. In the next stage of the study, sediment and flotate from the investigated samples were examined with PCR. Out of 132 sediment and flotate samples, bovine DNA was detected in eight (6.06%) and nine (6.82%) samples, respectively. On the basis of the results obtained with the use of the PCR, it is possible to state that the molecular biology methods can, at present, be used as supplementary tools for detection and identification of bovine MBM.

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“…Moreover, conventionaland real time-PCR are frequently used because of the sensitivity and specificity of these methods [11,[14][15][16] . The results of several studies have shown that these methods are also reliable for the analysis of heat-treated meat [12,17] ; however, processing conditions have been shown to have an effect on DNA fragment size and thus on the sensitivity of PCR detection [11,18] . In this study, we aimed to evaluate the individual and combined effects of pH and heat treatment on the identification of certain animal species in meat samples using PCR.…”

Section: Introductionmentioning

confidence: 99%

Proses Koşullarının Et Ürünlerinde Hayvan Türünün PCR İle Teşhisine Etkisi

Arun¹,

Çiftçioğlu²,

Sandikçi³

et al. 2014

Kafkas Univ Vet Fak Derg

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Although most consumers are sensitive about the origin of the meat they consume, adulteration of meat products is not uncommon. For this reason, the development of reliable methods for animal species identification in meat products is an important research priority for food scientists. Species-specific protein-and DNA-identification methods generally used for this purpose. ELISA and protein electrophoresis are used for protein, PCR is used for species-specific DNA identification. Because DNA is known to be more resistant to processing than protein, PCR methods are generally considered as more sensitive for processed foods. However, processing conditions may also degrade DNA resulting in decreased DNA quality and yield. In this study, the individual and combined effects of heat treatment and low pH on the identification of animal species in meat products by PCR were evaluated. Beef sausage mixtures containing two different amounts of meat from a secondary species (either poultry, pork, or horse) were prepared and were subjected to heat treatment (65°C, 85°C, and 121°C) and pH adjustment (5.2 and 6.2). PCR screening for the four animal species was performed using DNA extracts of these meat samples. The results showed that, the combined effect of high temperature and low pH significantly affects the detection limit of the PCR method. Nevertheless, even low levels of adulteration can still be detected fallowing heat treatment. Keywords: Animal species identification, PCR, Heat processing, DNA degradation, Low pH Proses Koşullarının Et Ürünlerinde Hayvan Türünün PCR İle Teşhisine Etkisi ÖzetBirçok tüketicinin tükettiği etin orijini konusunda hassasiyetinin bulunmasına karşın, et ürünlerinde yapılan hileler oldukça sıktır. Bu sebeple, çeşitli hayvan türlerinin et ürünlerinde tespit edilmesinde kullanılacak güvenilir metotların geliştirilmesi, gıda bilimcilerinin öncelikli çalışma konularındandır. Bu amaçla, türe spesifik protein yada DNA'nın tanımlanması yöntemleri kullanılır. Protein tanımlanması için ELISA ve protein elektroforezi kullanılırken, türe spesifik DNA tanımlanmasında PCR yönteminden yararlanılır. DNA'nın proses koşullarına daha dayanıklı olduğu bilindiğinden, PCR işlem görmüş ürünlerde daha hassas olarak kabul edilmektedir. Buna karşın, proses koşulları DNA üzerine de yıkımlandırıcı etki göstererek, DNA kalite ve miktarını zayıflatabilir ve böylece PCR metotlarının işlenmiş ürünlerde kullanımını sınırlandırabilir. Bu çalışma ile ısıl işlem ve düşük pH'nın bağımsız ve kombine etkisinin et ürünlerinde hayvan türünün PCR ile teşhisine olan etkisini incelenmiştir. Bu amaçla, iki farklı seviyede ikincil bir türe ait (tavuk, domuz yada at) et katılmış olan deneysel sığır sosis karışımları yaygın olarak kullanılan proses şartlarını temsil etmek üzere ısıl işleme (65°C, 85°C, and 121°C) ve pH ayarlamasına (5.2 and 6.2) tabi tutuldu. Bu karışımların DNA ektraktlarının, PCR ile tür tayini testleri gerçekleştirildi. Elde edilen sonuçlar, düşük pH ve ısıl işlemin kombine etkisinin tespit limiti üzerine olduk...

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Effect of method of cooking on identification of heat processed beef using polymerase chain reaction (PCR) technique

Cited by 128 publications

References 30 publications

Species Authentication of Dog, Cat, and Tiger Using Cytochrome β Gene

Species Authentication of Dog, Cat, and Tiger Using Cytochrome β Gene

DNA based approaches for the detection and identification of bovine meat and bone meal in feeds

Proses Koşullarının Et Ürünlerinde Hayvan Türünün PCR İle Teşhisine Etkisi

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