A two years old male cat domestic long hair was brought to the Tabby Pet Care clinic with the owner's complaint that the cat often scratches his right and left ears. The results of clinical examination showed scratching marks on the pinna, blackish yellow, wet, and smelly ear serumen on the ear canal. Investigations include microscopic examination of ear swabs and cerumen swab cytology. Ear swab examination results observed the presence of Otodectes cynotis and Otodectes cynotis eggs. The results of cytological examination of cerumen swabs using the rapid stain method were observed to have bacterial infections in the form of bacilli, coccus, and the presence of neutrophil infiltration. Based on clinical symptoms and the results of investigations, the patient was diagnosed with external otitis with a fausta prognosis. Treatment of otitis externa is carried out with topical therapy Ilium® ear drop. Therapy is carried out for seven days. On the eighth day the patient was declared cured and allowed to go home.
A female Asian palm civet (Paradoxurus hermaphroditus), three years old was carried for a medical checkup to Ruddy animal's clinic in Sidoarjo, East Java, Indonesia. The civet suffers enlargement of abdominal mammary glands, painless lump, asymmetric size (4.1 and 8.4 cm in diameter), and lacerated wound on the large one with severe haemorrhage. The unilateral mastectomy was conducted under anaesthesia to handles both haemorrhage and tumour mass. Following the surgery, the tumour mass was stored in 10% neutral buffer formalin for histopathology using Hematoxylin & Eosin (H&E) staining and immunohistochemistry against antibody, anti-CD4+ and CD8+, further, a blood sample collected before and after surgery (on days: 0, 7, 30, and 60) for representing the healing progress. The chemotherapy was given using the combination of oral cyclophosphamide and intravenous injection of vincristine. According to laboratory results, the final diagnosis was mixed mammary carcinosarcoma with minimal expression of CD8+, notwithstanding, it showed the better prognosis after surgery and chemotherapy.
Tujuan Penelitian ini adalah untuk mengetahui efektifitas ekstrak kulit mahkota dewa sebagai terapeutikadiabetes mellitus tipe 1 terhadap profil leukosit dan hemoglobin pada tikus yang diinduksi aloksan. jenis penelitian ini eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL). Sampel Hewan yang digunakan adalah tikus jantan umur 5-6 minggu sebanyak 24 ekor. Kelompok tikus dibagi menjadi tiga kelompok, yaitu kelompok kontrol negatif (diinduksi aloksan 0,2 ml/ip tanpa diobati/P0), kontrol positif: diinduksi aloksan 0,2 ml/ip dan diobati syrupus ekstrak kulit buah mahkota dewa 50% diberikan 1 kali sehari (P1), diinduksi aloksan 0,2 ml/ip dan diobati syrupus ekstrak kulit buah mahkota dewa 50% sebanyak 2 kali sehari (P2). Semua tikus diberikan perlakuan pengobatan kulit buah mahkota dewa 50% selama 10 hari. Pengamatan dilakukan pada hari ke-0, 5, 10. Parameter pengamatan adalah kandungan senyawa aktif tanaman, kadar glukosa darah, profil leukosit (neutrofil, basofil dan eusinofil) dan hemoglobin. Semua data yang diperoleh, dilakukan analisis statistik dengan menggunakan ANOVA dengan taraf kepercayaan 95% (=0,05). Hasil statistik menunjukkan bahwa pada tikus yang diobati syrupus kulit buah mahkota dewa 50% 2 kali sehari (P2) menunjukan hasil yang paling baik daripada kelompok P0 dan P1.Analisis statistik menunjukkan Fhitung > F tabel berarti terdapat perbedaan yang signifikan antara kelompok P2 dengan P0 dan P1 terhadap penurunan glukosa, peningkatan profil leukosit, dan nilai hemoglobin yang normal. Hasil penelitian ini menunjukan bahwa aktifitas syrupus kulit buah mahkota dewa50% yang diberikan dua kali sehari paling efektif digunakan sebagai terapeutika diabetes mellitus tipe-1 pada tikus putih yang diinduksi aloksan.
Tujuan penelitian ini adalah untuk menganalisis kadar Tumor necrosis Factor ɑ(TNF-α) dan derajat kerusakan hepar pada tikus putih (Rattus norvegicus) yang diinfeksi isolat Trypanosoma evansi. Penelitian ini adalah penelitian eksperimental dengan Rancangan Acak Lengkap (RAL). Sampel Hewan adalah tikus jantan umur 6 minggu sebanyak 30 ekor dan dibagi dalam 5 kelompok dengan pemberian infeksi secara subcutan (sc), yaitu Po: kelompok tikus kontrol diinjeksi NaCl fisiologis dosis 0,3 ml, pengambilan sampel darah dan organ hepar pada hari ke 7 pasca injeksi NaCl fisiologis. P1: kelompok tikus diinfeksi Trypanosoma evansi dosis 0,3 ml/sc, pengambilan sampel darah dan organ hepar pada hari pertama pasca infeksi Trypanosoma evansi. P2: kelompok tikus diinfeksi Trypanosoma evansi dosis 0,3 ml/sc, pengambilan sampel darah dan organ hepar pada hari ke 3 pasca infeksi Trypanosoma evansi. P3: kelompok tikus diinfeksi Trypanosoma evansi dosis: 0,3 ml/sc, pengambilan sampel darah dan organ hepar pada hari ke 5 pasca infeksi Trypanosoma evansi. P4: kelompok tikus putih diinfeksi Trypanosoma evansi dosis 0,3 ml/sc, pengambilan sampel darah dan organ hepar pada hari ke 7 pasca infeksi Trypanosoma evansi. Nilai Optical Density (OD) atau kadarTNF-αmenunjukkan p=0,0624 (p>0,05), mengalami penurunan dan tidak berbeda, dan tidak terdapat hubungan bermakna antara kelompok pada tikus putih yang diinfeksi Trypanosoma evansi isolat Sumbawa. Pemberian infeksi secara subkutan dapat menyebabkan kerusakan hepar berupa lesi degenerasi, nekrosis, dan portal inflamasi pada tikus putih yang diinfeksi Trypanosoma evansi isolat Sumbawa. Kesimpulan adalah kadar TNF-α menurun, mengakibatkan kerusakan hepar dan tingkat keganasan parasit Trypanosoma evansi isolat Sumbawa meningkat.
Reptile plays an essential role in human life and act as a reservoir of pathogenic bacteria. It became necessary because of some bacteria resistant against several antibiotics. This study aimed to evaluate the potency of Sansevieria masoniana (SM) leaf extract against isolated bacteria from the faeces of pet-reptile. A total of 129 fresh faecal samples were collected from the reptile communities in Surabaya on February 2018 until January 2019. The faeces obtained from 72 snakes, 43 lizards and 14 tortoises. The isolation was conducted using the Micro ID system. All the isolated bacteria were tested against several antibiotics using disc diffusion method, and SM extract using minimum inhibitory concentration test. The isolated bacteria were Aeromonas hydrophila (44.96%), Bacillus sp (32.55%), Enterobacter cloacae (40.31%), Enterococcus sp (82.17%), Escherichia coli (96.89%), Proteus sp (76.74%), Pseudomonas sp (48.83%), Salmonella enteritidis (55.03%), and Salmonella enterica arizonae (53.48%). Those isolated bacteria indicated various resistance patterns against several commercial antibiotics. The minimum concentration of SM extracts that potential to inhibit the colonisation of both resistant and susceptible isolated bacteria was 62.5 mg/mL. This study proved that SM extract potential to inhibit the colonisation of the isolated bacteria from faeces of pet-reptile, even though, several of those isolates resistant against several commercial antibiotics.
The purpose of this research aimed to determine the antibacterial effectiveness of the methanolic extract of dragon scales leaves (Drymoglossum piloselloides) against MRSA (Methicilin-resistant Staphylococcus aureus) and Eschericia coli bacteria in vitro using the diffusion method. This research used a population of Methicillin-resistant Staphylococcus aureus and Eschericia coli bacteria. The sample to be used is dragon scale leaf methanol extract. The method in this research used experimental with 6 treatments, namely dragon scale leaf methanol extract with concentrations of 45%, 55%, 65%, 75%, negative control (methanol) and positive control (chloramphenicol antibiotics). Data collection in this study was carried out by recording the diameter of the inhibition zone using a caliper (mm). The data obtained from the diameter of the inhibition zone of 75% extract variation resulted in an inhibition zone of 17.02 mm (in MRSA) and 8.47 mm (in E.coli). Chloramphenicol was found to be resistant to MRSA, but sensitive to E.coli. Data analysis used ANOVA test which statistically showed that there was a significant difference (P<0.05) in the diameter of the inhibition zone of the dragon scale leaf methanol extract against MRSA bacteria and there was a very significant difference (P<0.01) in the diameter Inhibition zone of dragon scale leaf methanol extract against Eschericia coli bacteria.
The purpose of this study was to determine the presence of Toxocara cati eggs in the feces of Domestic pet cats at the Surabaya Animal Clinic and the durability of Toxocara cati eggs with in vitro media. Total fecal samples taken were 30 cat feces from 5 animal clinics in Surabaya, then detected Toxocara cati eggs using an enlargement microscope 100 times, followed by counting eggs per gram of feces. The eggs used were approximately 200 eggs in each petri dish, then positive feces of Toxocara cati with various in vitro media using 0.9% NaCl, 1% PBS and 1% CMC for 24 hours and 48 hours. The results showed that two fecal samples positive Toxocara cati from 30 samples fecal and durability Toxocara cati eggs on medium in vitro over 24 showed 28% NaCl; PBS 68%; CMC 5% and at 48 hours showed 23.7% NaCl; PBS 58%; CMC 17.3%. Based on these results, the conclusion is two fecal (6,67 %) positive T.cati from 30 fecal samples, and the best medium in the test of the durability of Toxocara cati egg is 1% PBS.
Brucellosis (Keluron disease) is a strategic infectious disease with zoonotic potential, one of which is genus Brucella abortus. This research aims to detect Brucellosis in dairy cattle FH in Pagu District, Kediri Regency. This research uses two serological tests, namely the Rose Bengal Test (RBT) and the Complement Fixation Test (CFT) with descriptive analysis method. 30 cattle blood samples were taken from the coccigea vein. The blood was set aside at room temperature to form a serum. The serum that has been obtained is taken to the Department of Food and Livestock of Kediri Regency for checking the RBT. The remaining serum obtained was sent to BBVet Wates for CFT examination. The results obtained show negative results on both tests. But one of the samples with the code S9 had gone through an abortion. This might occur due to other factors such as genetic disorders, the presence of viruses such as BVD, toxic agents and live vaccines during pregnancy.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.