Potency of Sansevieria masoniana Extract against Antimicrobial Resistant Bacteria Isolated from Faeces of Pet - Reptile.

Abstract

Reptile plays an essential role in human life and act as a reservoir of pathogenic bacteria. It became necessary because of some bacteria resistant against several antibiotics. This study aimed to evaluate the potency of Sansevieria masoniana (SM) leaf extract against isolated bacteria from the faeces of pet-reptile. A total of 129 fresh faecal samples were collected from the reptile communities in Surabaya on February 2018 until January 2019. The faeces obtained from 72 snakes, 43 lizards and 14 tortoises. The isolation was conducted using the Micro ID system. All the isolated bacteria were tested against several antibiotics using disc diffusion method, and SM extract using minimum inhibitory concentration test. The isolated bacteria were Aeromonas hydrophila (44.96%), Bacillus sp (32.55%), Enterobacter cloacae (40.31%), Enterococcus sp (82.17%), Escherichia coli (96.89%), Proteus sp (76.74%), Pseudomonas sp (48.83%), Salmonella enteritidis (55.03%), and Salmonella enterica arizonae (53.48%). Those isolated bacteria indicated various resistance patterns against several commercial antibiotics. The minimum concentration of SM extracts that potential to inhibit the colonisation of both resistant and susceptible isolated bacteria was 62.5 mg/mL. This study proved that SM extract potential to inhibit the colonisation of the isolated bacteria from faeces of pet-reptile, even though, several of those isolates resistant against several commercial antibiotics.