Several sesquiterpene lactone were synthesized and their inhibitive activities on phospholipase A(2) (PLA(2)) from Bothrops jararacussu venom were evaluated. Compounds Lac01 and Lac02 were efficient against PLA(2) edema-inducing, enzymatic and myotoxic activities and it reduces around 85% of myotoxicity and around 70% of edema-inducing activity. Lac05-Lac08 presented lower efficiency in inhibiting the biological activities studied and reduce the myotoxic and edema-inducing activities around only 15%. The enzymatic activity was significantly reduced. The values of inhibition constants (K(I)) for Lac01 and Lac02 were approximately 740 μM, and for compounds Lac05-Lac08 the inhibition constants were approximately 7.622-9.240 μM. The enzymatic kinetic studies show that the sesquiterpene lactones inhibit PLA(2) in a non-competitive manner. Some aspects of the structure-activity relationships (topologic, molecular and electronic parameters) were obtained using ab initio quantum calculations and analyzed by chemometric methods (HCA and PCA). The quantum chemistry calculations show that compounds with a higher capacity of inhibiting PLA(2) (Lac01-Lac04) present lower values of highest occupied molecular orbital (HOMO) energy and molecular volume (VOL) and bigger values of hydrophobicity (LogP). These results indicate some topologic aspects of the binding site of sesquiterpene lactone derivatives and PLA(2).
This work presents densities, viscosities, interfacial tensions, and liquid−liquid equilibrium data for systems of interest in vegetable oil refining and biodiesel production, composed of soybean oil + commercial cis,cis-octadeca-9,12-dienoic acid (linoleic acid) + ethanol + water at 298.2 K with water mass fractions in the alcohol solvent (ethanol + water) of 0.05, 0.08, 0.11, and 0.14. Experimental phase compositions at equilibrium were correlated using the nonrandom two-liquid (NRTL) model. For all systems, the global deviations between calculated and experimental phase compositions were no greater than 0.0005. All physical properties were evaluated by empirical correlations as functions of phase compositions. The absolute relative deviations between calculated and experimental data were no greater than 6.36 %. Water content in the solvent presented a considerable effect on the physical properties of the systems and phase compositions at equilibrium, even more so on its interfacial tensions.
ResumoA disponibilidade de água em volumes expressivos e em qualidade incomparável são requisitos básicos para a operação e pleno funcionamento de unidades de hemodiálise. O presente trabalho avaliou a qualidade microbiológica em diferentes pontos de um sistema inovador de tratamento de água destinada a hemodiálise e que prioriza seu reuso, refletindo em importantes ganhos sob o ponto de vista ambiental assim como na racionalização de gastos em um Hospital filantrópico do interior de Minas Gerais. Os resultados confirmam excelência na qualidade microbiológica da água destinada a diálise atendendo aos requisitos e valores máximos permitidos estabelecidos pela legislação específica vigente (Portaria MS 2914/11 e RDC 11/2014).Palavras-chave: HEMODIÁLISE; SUSTENTABILIDADE, ÁGUA.
MICROBIOLOGICAL MONITORING IN INNOVATIVE AND SUSTAINABLE WATER TREATMENT SYSTEM DESTINED TO HEMODIALYSIS AbstractThe availability of large volumes of water in unparalleled quality are basics requirements for operation full of hemodialysis units. The present study assessed the microbiological quality in different points of an innovative system water treatment to hemodialysis that prioritizes your reuse with reflects in important gains under the environmental viewpoint as well as the rationalization of spending in a Hospital philanthropic of Minas Gerais state. The results
Background:
Numerous causes of infection in arthroplasties are related to biofilm formation
on implant surfaces. In order to circumvent this problem, new alternatives to prevent bacterial
adhesion biosurfactants-based are emerging due to low toxicity, biodegradability and antimicrobial
activity of several biosurfactants. We revised all patents relating to biosurfactants of applicability
in orthopedic implants.
Methods:
This work aims to evaluate the capability of a lipopeptide produced by Bacillus subtilis
ATCC 19659 isolates acting as inhibitors of the adhesion of Escherichia coli ATCC 25922 and
Staphylococcus aureus ATCC 29213 onto titanium and stainless steel surfaces and its antimicrobial
activity.
Results:
The adhesion of the strains to the stainless-steel surface was higher than that of titanium.
Preconditioning of titanium and stainless-steel surfaces with 10 mg mL-1 lipopeptide reduced the
adhesion of E. coli by up to 93% and the adhesion of S. aureus by up to 99.9%, suggesting the
strong potential of lipopeptides in the control of orthopedic infections. The minimal inhibitory
concentration and minimum bactericidal concentration were 10 and 240 µg mL-1 for E. coli and
S. aureus, respectively.
Conclusion:
The lipopeptide produced by Bacillus subtilis ATCC 19659 presented high biotechnological
application in human health against orthopedic implants infections.
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