Recent Patents on Impact of Lipopeptide on the Biofilm Formation onto Titanium and Stainless Steel Surfaces

Abstract

Background: Numerous causes of infection in arthroplasties are related to biofilm formation on implant surfaces. In order to circumvent this problem, new alternatives to prevent bacterial adhesion biosurfactants-based are emerging due to low toxicity, biodegradability and antimicrobial activity of several biosurfactants. We revised all patents relating to biosurfactants of applicability in orthopedic implants. Methods: This work aims to evaluate the capability of a lipopeptide produced by Bacillus subtilis ATCC 19659 isolates acting as inhibitors of the adhesion of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 29213 onto titanium and stainless steel surfaces and its antimicrobial activity. Results: The adhesion of the strains to the stainless-steel surface was higher than that of titanium. Preconditioning of titanium and stainless-steel surfaces with 10 mg mL-1 lipopeptide reduced the adhesion of E. coli by up to 93% and the adhesion of S. aureus by up to 99.9%, suggesting the strong potential of lipopeptides in the control of orthopedic infections. The minimal inhibitory concentration and minimum bactericidal concentration were 10 and 240 µg mL-1 for E. coli and S. aureus, respectively. Conclusion: The lipopeptide produced by Bacillus subtilis ATCC 19659 presented high biotechnological application in human health against orthopedic implants infections.

Production of biosurfactants by bacteria collected in soil contaminated by diesel oil on the Antarctic Continent

“…Droplet spreading is a consequence of the presence of surface active compounds or tensoative such as biosurfactants (Tugrul & Cansunar, 2005). Drop collapse analyses demonstrated the presence of biosurfactants due to increase the diameter of the droplet applied compared to the distilled water control (Pires et al, 2020). Recently, the screening of biosurfactant production from newly isolated Rhodotorula sp.…”

“…Biosurfactants can replace synthetic surfactants in a wide variety of industrial applications, such as detergents, foams, emulsifiers, solubilizers and wetting agents due to their characteristics such as low CMC values, ability to reduce surface/interfacial tension and emulsification properties (Barros et al, 2008;Nitschke & Silva, 2018;Hajimohammadi et al, 2018;Befkadu & Chen, 2018). In addition, biosurfactants can perform several biological properties such as fungicides, insecticides, antivirals, antibacterial, anti-biofilm adhesion, anticancer, and enzyme inhibition (Nithya et al, 2010;Harshada, 2014;Helmy et al, 2011;Varvaresou & Iakovou, 2015;Mnif & Ghribi, 2015;Clements et al, 2019;Pires et al, 2020).…”

Effect of DMPEI coating against biofilm formation on PVC catheter surface

“…In the case of anti-bio lm strategies in catheters are a) bactericidal agents; b) antifouling agents; c) bio lm disruptors; d) benign bio lms, including E. coli 83972 and E. coli Nissle 1917 bio lms (Zhu et al 2019). In this context, avoiding initial cell adhesion using molecules on the surface could be a relevant strategy (Keum et al 2017;Mahata et al 2017;Pires et al 2019). We highlighted the use of N, N-dodecyl, methyl polyethylenimine (DMPEI).…”

Extração De Proteínas De Sementes

“…A partir dessa concentração há a formação de micelas esféricas, sendo assim, este ponto denominado de concentração micelar crítica (CMC), corresponde à mínima concentração de surfactante necessária para que a tensão superficial seja reduzida ao máximo (Fig. 17) (PIRES et al, 2020;. A CMC é um dos índices mais utilizados para a avaliação da atividade surfactante.…”