Absence of antibacterial, anti-candida, and anti-dengue activities of a surfactin isolated from Bacillus subtilis

Lima, William Gustavo; Parreira, Adriano Guimarães; Nascimento, Lídia Anitta Alves; Leonel, Camyla Alves; Andrade, Jéssica Tauany; Palumbo, Juliana Maria Campos; Soares, Adriana Cristina; Granjeiro, Paulo Afonso; Ferreira, Jaqueline Maria Siqueira

doi:10.4103/jpnr.jpnr_11_17

Cited by 10 publications

(3 citation statements)

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“…The fractional inhibitory concentration (FIC) of ATO combinations with FLU, ITR, KET and VOR against the representative isolates was evaluated by checkerboard microdilution method (Lima et al 2018). Brie y, two-fold serial dilutions of ATO (50 μl) and the tested antifungal agent (50 μl) were dispensed in horizontal and vertical orientations, respectively.…”

Section: In Vitro Synergy Assaymentioning

confidence: 99%

WITHDRAWN: In Vitro Study On The Potential Fungicidal Effects of Atorvastatin In Combination With Some Azole Drugs Against Multidrug Resistant Candida Albicans

Mahmoud

Faraag

El-Wafa

2021

Preprint

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The resistance of Candida albicans to azole drugs represents the great global challenge. This study investigates the potential fungicidal effects of atorvastatin (ATO) combinations with fluconazole (FLU), itraconazole (ITR), ketoconazole (KET) and voriconazole (VOR) against thirty-four multidrug-resistant (MDR) C. albicans using checkerboard and time-kill methods. Results showed that 94.12% of these isolates were MDR to ≥ two azole drugs, whereas 5.88% of them were susceptible to azole drugs. The tested isolates exhibited high resistance rates to FLU (58.82%), ITR (52.94%), VOR (47.06 %) and KET (35.29 %), whereas only three representative (8.82%) isolates were resistant to all tested azoles. Remarkably, the inhibition zones of these isolates were increased at least two-fold with the presence of ATO, which interacted in a synergistic (FIC index ≤ 0.5) manner with tested azoles. In silico docking study of ATO and the four azole drugs were performed against the Lanosterol 14-alpha demethylase enzyme (ERG11) of C. albicans. Results showed that the mechanism of action of ATO against C. albicans is similar to that of azole compounds, with docking score (-4.901) lower than azole drugs (> - 5.0) due to the formation a single H-bond with Asp 225 and a pi-pi interaction with Thr 229. Importantly, ATO combinations with ITR, VOR and KET achieved fungicidal effects (≥ 3 Log10 cfu/ml reduction) against the representative isolates, whereas a fungistatic effect (≤ 3 Log10 cfu/ml reduction) was observed with FLU combination. Thus, the combination of ATO with azole drugs could be promising options for treating C. albicans infection.

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Section: In Vitro Synergy Assaymentioning

confidence: 99%

WITHDRAWN: In Vitro Study On The Potential Fungicidal Effects of Atorvastatin In Combination With Some Azole Drugs Against Multidrug Resistant Candida Albicans

Mahmoud

Faraag

El-Wafa

2021

Preprint

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“…It is noteworthy that the strain of C. glabrata used in this study is highly resistant to azoles drugs, such as ketoconazole, fluconazole, and itraconazole. 41 On the other hand, complex 1 exhibited unsatisfactory antibacterial activity in relation to streptomycin, as it was only moderately active against S. aureus, S. saprophyticus and P. aeruginosa (MICs in the ranges from 260 to 530 µmol L -1 ). Complexes 2 and 3 demonstrated activity against the three yeasts, all of them with MIC values equal to 530 µmol L -1 ( Table 2).…”

Section: Resultsmentioning

confidence: 99%

SYNTHESIS, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF NEW Cu (II), Co (II) AND Sn (II) COMPLEXES WITH THE SODIUM HYDROTRIS(2-MERCAPTOTHIAZOLYL)BORATE LIGAND

Ferreira¹,

Andrade²,

Lima³

et al. 2020

Quim. Nova

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Recebido em 27/11/2019; aceito em 05/02/2020; publicado na web em 16/04/2020 The emergence of antibiotic resistance among microbial pathogens is a serious public health problem, resulting in a constant need of discovering new antibiotics. For this purpose, we synthetized and determined the antimicrobial activity of copper (Cu), cobalt (Co), and tin (Sn) complexes coordinated with scorpionate ligand in a tridentate mode against microorganisms of clinical importance. New complexes with the sodium hydrotris(2-mercaptothiazolyl)borate ligand were characterized by physicochemical and spectroscopic techniques (UV-vis, IR, 1 H and 13 C NMR). Moreover, their antimicrobial activity was evaluated using microdilution broth method. Cytotoxicity was determined in Vero cells and the selectivity index (SI) was calculated. The coordination with metals increased the ligand antimicrobial activity, which showed no activity when evaluated alone (MIC>1,080 µM). Cu-complex (1) had activity against Candida albicans and C. krusei, with MICs of 130 and 66 µM, respectively. The compounds had low toxicity on Vero cells and chemo-informatics assays corroborated a low systemic toxic effect. The SI revealed that the compound 1 was 16 times more selective for C. krusei and 8 times more selective for C. albicans in relation to Vero cells. The Cu-complex stands out as a potential prototype for the development of new antifungal agents.

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“…The antifungal mechanism of surfactin is mainly to destroy the fungal lipid membrane and lyse the pathogenic fungi (Lima et al 2018) and down-regulate the expression of several genes involved in ergosterol synthesis, morphogenesis or metabolism such as glycolysis, ethanol and fatty acid biosynthesis, thus affecting the physiological functions of the pathogenic fungi (Jakab et al 2022) while the mechanism of iturin and fengycin is to change the surface tension of fungal cell membrane; this results in the leakage of K + and other important ions in the cell, eventually leading to fungal death (Banerjee et al 2022;Lei et al 2019). Moreover, cell wall lyases including cellulase, glucanase, protease and chitinase produced by Bacillus spp.…”

Section: Identification Of Endophytic Bacteriamentioning

confidence: 99%

Antimicrobial activities of endophytic bacteria isolated from Ageratum conyzoides Linn.

et al. 2023

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Abstract. Boonman N, Chutrtong J, Wanna C, Boonsilp S, Chunchob S. 2023. Antimicrobial activities of endophytic bacteria isolated from Ageratum conyzoides Linn. Biodiversitas 24: 1971-1979. Endophytic bacteria were isolated from various parts of Ageratum conyzoides Linn. Total 35 isolates were obtained which were consisting of eight isolates from the roots (AconR1-AconR8), nine isolates from the stems (AconS1-AconS9), seven isolates from the leaves (AconL1-AconL7) and 11 isolates from the flowers (AconF1-AconF11). These endophytic bacteria were examined for their antimicrobial activities against human pathogenic bacteria and fungi. The AconR2 and AconR4 inhibited the growth of Shigella flexneri and Salmonella enterica ser. typhi, whereas Escherichia coli was only inhibited by AconR2. For the antifungal activities assay, all isolated endophytic bacteria revealed no effect on Candida albicans, while six endophytic isolates exhibited more than 80% mycelial growth inhibition against Microsporum canis. These highly effective isolates were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). The Bruker scores revealed that AconR2 and AconR4 were identified as Pseudomonas aeruginosa, AconS1 and AconS6 were classified as Enterobacter asburiae, Acon L5 and Acon F9 were identified as Bacillus cereus. The results indicated that these endophytic bacteria isolated from A. conyzoides effectively inhibited the growth of human pathogenic filamentous fungi and showed promise for further development as novel antifungal agents.

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Absence of antibacterial, anti-candida, and anti-dengue activities of a surfactin isolated from Bacillus subtilis

Cited by 10 publications

References 1 publication

WITHDRAWN: In Vitro Study On The Potential Fungicidal Effects of Atorvastatin In Combination With Some Azole Drugs Against Multidrug Resistant Candida Albicans

WITHDRAWN: In Vitro Study On The Potential Fungicidal Effects of Atorvastatin In Combination With Some Azole Drugs Against Multidrug Resistant Candida Albicans

SYNTHESIS, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF NEW Cu (II), Co (II) AND Sn (II) COMPLEXES WITH THE SODIUM HYDROTRIS(2-MERCAPTOTHIAZOLYL)BORATE LIGAND

Antimicrobial activities of endophytic bacteria isolated from Ageratum conyzoides Linn.

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