Flavonoids can provide antioxidant effects by preventing the formation of ROS, directly capture ROS, protect lipophilic antioxidants and stimulate the increase of enzymatic antioxidants. Flavonoids are phenolic compounds that are widely found in medicinal plants, one of which is Gyrinops versteegii leaves. In this research to determine the potential of flavonoids in water extract Gyrinop versteegii as one source of natural antioxidants was investigated. This research begins with maceration of Gyrinops versteegii leaves with some solvents such as ethyl acetate, ethanol, methanol and water. Each of the extracts obtained measured the total content of Phenol. The extract, which had the highest total phenol content, measured the total flavonoid content and antioxidant capacity. The active extract as antioxidant was further isolated and identified its flavonoid content. Flavonoids obtained measured antioxidant capacity in vitro. Total phenol (mg GAE/100 g) of ethyl acetate extract = 443, ethanol extract =1.510, methanol extract = 6.069 and water extract = 14.979, total flavonoid contens = 2298, 977 mg QE/100 gram, containing phenol, flavonoid, tannin, alkaloid and steroid compounds. Antioxidant capacity with IC50 = 3,45 ppm (5 min.) and 3,05 (60 min.). Identification of isolates with UV-Vis spectroscopy showed 2 absorption bands namely band I at 352 nm and band II at 256 nm. Addition of AlCl3 / HCl shear reagent showed band I undergoing a 2 nm batochromic shift. These results indicate that the resulting flavonoid is suspected to be a flavonoid group of flavonol substituted -OH group at C-5 or 5-hydroxy-flavonol. and its antioxidant capacity or IC50 = 17,14 ppm. These results indicate that the isolated flavonoid has very strong antioxidant activity and is potentially developed as a natural antioxidant.
Phenols and flavonoids compounds in their activities as antioxidants can reduce blood glucose levels (hypoglycemic) through a mechanism to inhibit cell damage at pancreatic Langerhans gland, inhibit glucose absorption in the intestine and cell resistance to insulin. Preliminary analysis shows that total phenol and flavonoid contens is 2099,5025 mg GAE / 100 g, and 8133,322 mg QE / 100 gram, Antioxidant capacity with IC50 = 12.58 mg / mL. LD50 = 6,355,3536 gram. This results revealad that the water extract of Gyrinops versteegii leaves can be developed as an alternative to natural antioxidant, safe for consumption and can reduce blood glucose in hyperglycemic Wistar rats. The results of the measurement of the effect of decreasing blood glucose content showed that the water extract of Gyrinops versteegii leaves at doses of 100, 200 and 400 mg / kgBW significantly (p <0.01) could reduce blood glucose contents in hyperglycemic Wistar rats. The activity of reducing blood glucose levels (hypoglycemic) with oral doses of water extract of Gyrinops versteegii leaves was 100,200 and 400 mg / kgBW was 53.6% (53.6 ± 5.8), 58.9% (58.9 ± 8.6 ) and 75.8% (75.8 ± 1.3). High contents of phenol and flavonoids allegedly a factor that causes water extract of Gyrinops versteegii leaves to reduce blood glucose levels in hyperglycemic Wistar rats.
Induction of apoptosis and antiangiogenesis effects of Pinostrobin from Kaempferia pandurata Roxb against Fibrosarkoma mice results benzopiren induction has been done. Examination or surgery begins taking tissue fibrosarcoma in mice infected and weigh fibrosarcoma obtained. Fibrosarcoma tissues were then stored in containers that have contained 10 % formalin. Weighing results showed that the concentration of pinostrobin oral 80 mg / kg can inhibit the growth of fibrosarcoma with a gram weight of 68.62 % and a cancer drug (control +) there is resistance 95.95 % compared to the negative control which is only given orally CMC-Na, this means pinostrobin potentially be developed as a cancer chemotherapy. 20 Adi Parwata et al. Furthermore done shooting patohistologi tissue fibrosarcoma with HE staining with a light microscope with 400x magnification. The results obtained showed many chromatin (polikromatin) which prove the damage caused by having fibrosarcoma cells. Immunohistochemical assay showed oral pinostrobin concentration 80 mg / kg body weight can increase the expression of p53 to apoptosis induction could take place and the decreased expression of VEGF angiogenesis which proves the existence of barriers.
Gyrinops versteegii leaves are high in flavonoids, which have a variety of biological and pharmacological properties. The focus of this research was to determine the antioxidant and antibacterial activity of flavonoids in Gyrinops versteegii leaf water extracts. In this study, extraction was by maceration and partitioning, separation and purification was by chromatography, identification of compounds was carried out using UV-Vis and FTIR spectrophotometers, and antioxidant activity was measured with the DPPH method and antibacterial activity with the diffusion disc method. Flavonoids were thereby isolated. The ethyl acetate extract had the highest total flavonoid content, with 186.976 mg QE/100 g. With inhibition zone diameters of 19 and 13 mm, ethyl acetate extract was the strongest extract in inhibiting the growth of S. aureus and E. coli in the antibacterial activity test. Flavonoids were identified as flavonols after isolation, phytochemical screening, and UV-Visible and FTIR spectroscopic identification. The isolated flavonols had potent antioxidant activity (IC50 = 60.27 ppm) as well as potent antibacterial activity (inhibition zone diameters of 33.82 mm for S. aureus and 23.22 mm for E. coli). The findings therefore showed that tea made from Gyrinops versteegi leaves contains the flavonoid flavonol, which has antibacterial and antioxidant properties. Keywords: Gyrinops versteegii, Flavonol, DPPH, antibacterial and antioxidant
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